Abstract: Entomopathogenic fungi useful in the control of insects is prepared by culturing mycelia in a suitable medium, harvesting the growing mycelia, treating the mycelia with a protective agent, drying the treated mycelia and grinding and storing the dried product.

Abstract: Product of Entomophthora resting spores was increased by 50 to 70% by modifying an egg yolk media with a particular maltose agar. Germination of the resisting spores at levels up to 100% was obtained by preconditioning harvested spores by treatment with 95% ethanol, high speed blending, sonication, or a combination of ethanol treatment and high speed blending. Germination of spores which had been dried and stored but not pretreated as above was greatly increased by exposure to an atmosphere of 95% ethanol. In addition, treatment by the processes of this invention resulted in the production of a spore stage not previously observed or reported. Spores thus produced have been termed germ conidia.

Abstract: Production of Entomophthora resting spores was increased by 50 to 70% by modifying an egg yolk media with a particular maltose agar. Germination of the resting spores at levels up to 100% was obtained by preconditioning harvested spores by treatment with 95% ethanol, high speed blending, sonication, or a combination of ethanol treatment and high speed blending. Germination of spores which had been dried and stored but not pretreated as above was greatly increased by exposure to an atmosphere of 95% ethanol. In addition, treatment by the processes of this invention resulted in the production of a spore stage not previously observed or reported. Spores thus produced have been termed germ conidia.