Patents by Inventor Richard Somberg
Richard Somberg has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9631225Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.Type: GrantFiled: October 14, 2014Date of Patent: April 25, 2017Assignee: PROMEGA CORPORATIONInventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
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Publication number: 20150337359Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.Type: ApplicationFiled: October 14, 2014Publication date: November 26, 2015Inventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
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Patent number: 8859220Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.Type: GrantFiled: January 25, 2013Date of Patent: October 14, 2014Assignee: Promega CorporationInventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
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Patent number: 8603767Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.Type: GrantFiled: January 25, 2013Date of Patent: December 10, 2013Assignee: Promega CorporationInventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
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Patent number: 8487078Abstract: Compositions, methods, and kits for detecting and monitoring kinase, phosphatase and protein post-translational modification activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase, phosphatase or other protein post-translational modification alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase, phosphatase or other protein post-translational modification activity are also described.Type: GrantFiled: October 4, 2011Date of Patent: July 16, 2013Assignee: Life Technologies CorporationInventors: Tony Klink, Jane Beebe, David Lasky, Karen Kleman-Leyer, Richard Somberg
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Patent number: 8361739Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.Type: GrantFiled: June 21, 2010Date of Patent: January 29, 2013Assignee: Promega CorporationInventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
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Publication number: 20120083427Abstract: Compositions, methods, and kits for detecting and monitoring kinase, phosphatase and protein post-translational modification activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase, phosphatase or other protein post-translational modification alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase, phosphatase or other protein post-translational modification activity are also described.Type: ApplicationFiled: October 4, 2011Publication date: April 5, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Tony KLINK, Jane A. Beebe, David A. Lasky, Karen M. Kleman-Leyer, Richard Somberg
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Patent number: 8067536Abstract: Compositions, methods, and kits for detecting and monitoring kinase, phosphatase and protein post-translational modification activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase, phosphatase or other protein post-translational modification alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase, phosphatase or other protein post-translational modification activity are also described.Type: GrantFiled: March 22, 2010Date of Patent: November 29, 2011Assignee: Life Technologies CorporationInventors: Tony Klink, Jane Beebe, David Lasky, Karen Kleman-Leyer, Richard Somberg
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Publication number: 20110081670Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.Type: ApplicationFiled: June 21, 2010Publication date: April 7, 2011Applicant: PROMEGA CORPORATIONInventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
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Publication number: 20100240080Abstract: Compositions, methods, and kits for detecting and monitoring kinase, phosphatase and protein post-translational modification activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase, phosphatase or other protein post-translational modification alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase, phosphatase or other protein post-translational modification activity are also described.Type: ApplicationFiled: March 22, 2010Publication date: September 23, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Tony A. Klink, Jane A. Beebe, David A. Lasky, Karen M. Kleman-Leyer, Richard Somberg
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Patent number: 7741067Abstract: A method and kit is provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.Type: GrantFiled: December 23, 2003Date of Patent: June 22, 2010Assignee: Promega CorporationInventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
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Patent number: 7727752Abstract: Compositions, methods, and kits for detecting and monitoring kinase, phosphatase and protein post-translational modification activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase, phosphatase or other protein post-translational modification alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase, phosphatase or other protein post-translational modification activity are also described.Type: GrantFiled: July 14, 2006Date of Patent: June 1, 2010Assignee: Life Technologies CorporationInventors: Tony A. Klink, Jane A. Beebe, David A. Lasky, Karen M. Kleman-Leyer, Richard Somberg
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Patent number: 7700310Abstract: Methods and kits for detecting transferase activity in a sample by measuring ATP using a composition comprising an ATP-dependent bioluminescence-generating enzyme, a luminogenic molecule and one or more transferase quenching agents.Type: GrantFiled: September 5, 2003Date of Patent: April 20, 2010Assignee: Promega CorporationInventors: Richard Somberg, Said A. Goueli
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Publication number: 20100021949Abstract: Methods and kits for detecting transferase activity in a sample by measuring ATP using a composition comprising an ATP-dependent bioluminescence-generating enzyme, a luminogenic molecule and one or more transferase quenching agents.Type: ApplicationFiled: September 17, 2009Publication date: January 28, 2010Applicant: PROMEGA CORPORATIONInventors: Richard Somberg, Said A. Goueli
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Patent number: 7619059Abstract: Compositions, methods, and kits for detecting and monitoring post-translational modification activities, including kinase or phosphatase activities, are described. The compositions typically include a peptide, a first detectable moiety, a first binding member, and a protease cleavage site. Modification of a composition by a post-translational modification enzyme, such as a kinase or phosphatase, alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition when it is associated noncovalently with a probe composition that includes a second binding member and a second detectable moiety. Panel assays for determining substrates or modulators of enzymatic activities are also described.Type: GrantFiled: September 9, 2004Date of Patent: November 17, 2009Assignee: Life Technologies CorporationInventors: Kevin R. Kupcho, Kurt Vogel, Elizabeth A. Werner, Jane A. Beebe, Tony A. Klink, David A. Lasky, Karen M. Kleman-Leyer, Richard Somberg
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Patent number: 7582461Abstract: Compositions, methods, and kits for detecting and monitoring kinase or phosphatase activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase or phosphatase alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase or phosphatase activity are also described.Type: GrantFiled: July 29, 2004Date of Patent: September 1, 2009Assignee: Life Technologies CorporationInventors: Elizabeth A. Werner, Tony A. Klink, Jane A. Beebe, David A. Lasky, Karen M. Kleman-Leyer, Richard Somberg
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Publication number: 20070059787Abstract: Compositions, methods, and kits for detecting and monitoring kinase, phosphatase and protein post-translational modification activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase, phosphatase or other protein post-translational modification alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase, phosphatase or other protein post-translational modification activity are also described.Type: ApplicationFiled: July 14, 2006Publication date: March 15, 2007Applicant: Invitrogen CorporationInventors: Tony Klink, Jane Beebe, David Lasky, Karen Kleman-Leyer, Richard Somberg
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Publication number: 20050170442Abstract: Compositions, methods, and kits for detecting and monitoring post-translational modification activities, including kinase or phosphatase activities, are described. The compositions typically include a peptide, a first detectable moiety, a first binding member, and a protease cleavage site. Modification of a composition by a post-translational modification enzyme, such as a kinase or phosphatase, alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition when it is associated noncovalently with a probe composition that includes a second binding member and a second detectable moiety. Panel assays for determining substrates or modulators of enzymatic activities are also described.Type: ApplicationFiled: September 9, 2004Publication date: August 4, 2005Inventors: Kevin Kupcho, Kurt Vogel, Elizabeth Werner, Jane Beebe, Tony Klink, David Lasky, Karen Kleman-Leyer, Richard Somberg
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Publication number: 20050054573Abstract: Compositions, methods, and kits for detecting and monitoring kinase or phosphatase activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase or phosphatase alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase or phosphatase activity are also described.Type: ApplicationFiled: July 29, 2004Publication date: March 10, 2005Inventors: Elizabeth Werner, Tony Klink, Jane Beebe, David Lasky, Karen Kleman-Leyer, Richard Somberg
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Publication number: 20050026171Abstract: A method and kit is provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minize interference by at least about 10% relative to an assay not having tolerance enhancement agent.Type: ApplicationFiled: December 23, 2003Publication date: February 3, 2005Inventors: Erika Hawkins, James Cali, Samuel Ho, Martha O'Brien, Richard Somberg, Robert Bulleit, Keith Wood