Abstract: A reusable linker arm for solid support oligonucleotide synthesis, the linker arm comprising formula (a) wherein Z is a linker moiety and T is an organic radical.

Abstract: A process for production of the two or more molecules of interest. The present process may be advantageously used to produce two, three, four or more molecules of interest (the same or different from one another) on a single support material. A preferred embodiment comprises use of the present process to produce two or more oligonucleotides of interest. The process can be readily extended to other applications such combinatorial chemistry, peptide synthesis and the like. Various novel intermediates in the process are also disclosed.

Abstract: A novel approach for combining the ease of cleavage of carboxylic acid linker arms with the single phosphoramidite coupling chemistry of the universal supports useful in oligonucleotide synthesis. There is disclosed a new class of phosphoramidite reagents, linker phosphoramidites, which contain a bifunctional linker arm with a protected nucleoside linked through a 3′-ester bond on one end and a reactive phosphoramidite group or other phosphate precursor group on the other end—see FIGS. 2 and 3. The phosphoramidite group on the linker phosphoramidite may be activated under the same conditions and has similar reactivity as conventional nucleoside-3′-phosphoramidite reagents lacking the intermediate linker arm. The 3′-ester linkage contained within the linker phosphoramidite has similar properties to the linkages on prederivatized supports.

Abstract: A solid support for oligonucleotide synthesis is disclosed. The solid support has formula (1) wherein: R.sup.8 is selected from the group consisting of a substituted or unsubstituted C.sub.1 -C.sub.20 alkyl group, a substituted or unsubstituted C.sub.5 -C.sub.30 aryl group and a substituted or unsubstituted C.sub.5 -C.sub.40 alkylaryl group; X.sup.3 and X.sup.4 are the same or different and are selected from the group consisting of --O--, --S--, --S(O).sub.2 -- and --N(R.sup.12)--; R.sup.12 is selected from the group consisting of a substituted or unsubstituted C.sub.1 -C.sub.20 alkyl group, a substituted or unsubstituted C.sub.5 -C.sub.30 aryl group and a substituted or unsubstituted C.sub.5 -C.sub.40 alkylaryl group; and Y is selected from the group consisting of: --CH.sub.2 --CH.sub.2 --; --CH.sub.2 --; --CH.sub.2 --O--CH.sub.2 --; --CH.sub.2 --CH.sub.2 --CH.sub.2 --; --CH.dbd.CH--; --CH.dbd.C(CH.sub.3)--; --C(CH.sub.3).dbd.C(CH.sub.3)--; --CH.sub.2 --C(.dbd.CH.sub.2)--; and --CH.sub.2 --S--CH.sub.

Abstract: A linker arm for solid support oligonucleotide synthesis, the linker arm comprising formula (1), wherein: X.sup.1 is selected from the group consisting of --O--, --S--, --S(O).sub.2 --, --C(O)-- and --N(R.sup.12)--; R.sup.12 is selected from the group comprising hydrogen, a substituted or unsubstituted C.sub.1 -C.sub.20 alkyl group, a substituted or unsubstituted C.sub.5 -C.sub.30 aryl group and a substituted or unsubstituted C.sub.5 -C.sub.40 alkaryl group, X.sup.3 is --O-- or --N(H)--; R.sup.1, R.sup.2, R.sup.3, R.sup.4 and R.sup.5 are the same or different and are selected from the group consisting of hydrogen, halide, a substituted or unsubstituted C.sub.1 -C.sub.20 alkyl group, a substituted or unsubstituted C.sub.5 -C.sub.30 aryl group and a substituted or unsubstituted C.sub.5 -C.sub.40 alkylaryl group; n is 0, 1 or 2; and one of A' and B' is selected from the group consisting of hydrogen, halide, a substituted or unsubstituted C.sub.1 -C.sub.20 alkyl group, a substituted or unsubstituted C.sub.5 -C.