Abstract: Recombinant oleaginous alga that include one or more heterologous genes that increase the ability of the alga to use one or more natural saccharides such as cellulosic or hemicellulosic sugars for algal growth are described. The recombinant oleaginous algae are transformed to include one or more genes expressing sugar metabolizing enzymes or sugar transporting proteins, along with suitable control elements. Use of natural saccharides as a carbon source can allow the algae to produce biofuel precursors in a relatively efficient manner. Processes for preparing the alga, growing the alga, and extracting the biofuel precursors from the alga are also described.

Abstract: The present invention generally relates to fluorescent resonance energy transfer protein compounds and methods for using such compounds as biosensors. The present invention also relates to one or more nucleic acids for encoding the protein compounds, vectors containing the nucleic acids, cells transformed by the vectors, and methods for making and using the foregoing compositions.

Abstract: Methods for increasing the levels of lipids in oleaginous algae are described. Lipid levels in algae can be increased by stress, such as nutrient stress, after which the lipid can be harvested from the algae using a non-destructive extraction process. The stress may be provided in a periodic or “pulsed” fashion. Lipid levels in oleaginous algae can also be increased using simulated stress by treating the algae with a chemical inhibitor or by using recombinant technology to insert a sequence expressing a protein such as a nitrate reductase inhibitor that is expressed when a stressed state is desired. A method for maintaining the temperature and water levels of algae ponds using buoyant spheres is also described.

Abstract: Embodiments of the present invention utilize rationale genetic and chemical engineering strategies to achieve even greater efficiencies in biofuel production from microalgae. These increased efficiencies may be achieved through the application of targeted and well-designed chemical and genetic engineering methods disclosed herein. The exemplary embodiments focus on increasing single cell oil yields, increased algal culture densities, and increased efficiencies in oil production. Individually or in combination, exemplary embodiments may reduce the cost to produce a barrel of biofuel to enable commercial viability.

Abstract: Embodiments of the present invention includes an apparatuses, compositions, and methods utilizing mechanical and chemical engineering strategies to achieve even greater efficiencies in biofuels production from oleaginous organisms. These increased efficiencies may be achieved through the application of targeted and well-designed chemical and mechanical engineering methods disclosed herein to achieve a non-destructive extraction process (NDEP).

Abstract: Delivery systems and methods are provided for delivering a biologically active protein to a host animal. The systems and methods provided include obtaining an algal cell transformed by an expression vector, the expression vector comprising a nucleotide sequence coding for the biologically active protein, operably linked to a promoter. In one illustrated embodiment, the biologically active protein is an antigenic epitope and upon administration to the animal the algal cell induces an immune response in the host animal.

Abstract: Delivery systems and methods are provided for delivering a biologically active protein to a host animal. The systems and methods provided include obtaining an algal cell transformed by an expression vector, the expression vector comprising a nucleotide sequence coding for the biologically active protein, operably linked to a promoter. In one illustrated embodiment, the biologically active protein is an antigenic epitope and upon administration to the animal the algal cell induces an immune response in the host animal.

Abstract: Delivery systems and methods are provided for delivering a biologically active protein to a host animal. The systems and methods provided include obtaining an algal cell transformed by an expression vector, the expression vector comprising a nucleotide sequence coding for the biologically active protein, operably linked to a promoter. In one illustrated embodiment, the biologically active protein is an antigenic epitope and upon administration to the animal the algal cell induces an immune response in the host animal.

Abstract: Delivery systems and methods are provided for delivering a biologically active protein to a host animal. The systems and methods provided include obtaining an algal cell transformed by an expression vector, the expression vector comprising a nucleotide sequence coding for the biologically active protein, operably linked to a promoter. In one illustrated embodiment, the biologically active protein is an antigenic epitope and upon administration to the animal the algal cell induces an immune response in the host animal.