Abstract: According to the present invention there is disclosed a system and a method for detecting the presence of human fecal contamination on objects, such as a protein source, a worker's hands, work surfaces, tools or utensils. In one embodiment, there is included a supporting structure which supports a diffuse light source, the light source emitting light having a wavelength from the blue portion of the electromagnetic spectrum effective to elicit fluorescence from the fecal matter at wavelengths in the 600 nm to 800 nm range. A third wavelength of interest in the 600 nm to 800 nm range is also used as a reference control. Thereafter the wavelengths are combined in a series of mathematical steps to develop an algorithm or process to detect the presence of the human fecal material.