Abstract: Disclosed are a method for computer-assisted evaluation of biomarkers in a tissue sample as well as a corresponding system and computer program. The method includes the following steps: providing an image of the tissue sample in which cell nuclei of the tissue as well as biomarkers are represented, automatically determining for a plurality of locations or regions within the image a blood vessel probability that represents a probability that the image at the corresponding location or region represents a blood vessel, automatically determining an assumed blood vessel location within the image based on a distribution of blood vessel probability with respect to the locations or regions within the image, automatically selecting biomarkers represented in the image at locations outside the assumed blood vessel location but within a threshold distance from the assumed blood vessel location, and automatically evaluating the selected biomarkers.

Abstract: Disclosed are a method for computer-assisted evaluation of biomarkers in a tissue sample as well as a corresponding system and computer program. The method comprising the following steps: providing an image of the tissue sample in which cell nuclei of the tissue as well as biomarkers are represented, automatically determining for a plurality of locations or regions within the image a blood vessel probability that represents a probability that the image at the corresponding location or region represents a blood vessel, automatically determining an assumed blood vessel location within said image based on a distribution of blood vessel probability with respect to said locations or regions within the image, automatically selecting biomarkers represented in said image at locations outside the assumed blood vessel location but within a threshold distance from said assumed blood vessel location, and automatically evaluating the selected biomarkers.

Abstract: The invention relates to a method for end-titre determination in the determination of antibodies against nuclear and cytoplasmic antigens in human sera by means of an indirect immunofluorescence assay. The invention further relates to a kit for in vitro diagnosis for determining antibodies against nuclear and cytoplasmic antigens in human sera by means of an indirect immunofluorescence assay and a computer program for evaluation and for determination of the end titre within the framework of said method.

Abstract: The invention relates to a method comprising the following steps: a) use of a support which has at least two different microparticle populations immobilized thereon; b) measuring the fluorescence of the support from step a) with an optical resolution 1, said resolution 1 permitting differentiation of microparticle singlets, doublets, triplets, multiplets and monolayers and determination of the localized position of individual immobilized microparticles; c) contacting the support from step a) with the sample to be analyzed; d) performing at least one additional measurement of the fluorescence of the support during or after contacting in accordance with step c) with a resolution 2; e) assigning the fluorescence values measured with resolution 2 to the individual microparticle singlets, doublets, triplets, multiplets and monolayers locally identified on the support in accordance with step b) and assigned to a particular acceptor molecule population; f) determining the change in fluorescence.