Abstract: A cell culture method includes performing adherent culture of cells on a coat layer layered on a surface of a cell culture container by placing a culture medium and the cells in the cell culture container. The coat layer includes a layer (i) that includes a layer (i-1) including gelatin or casein and a layer (i-2) that includes a polycationic material and a layer (ii) that is layered on the layer (i) and includes a cell adhesion factor. The layer (ii) is disposed at a position at which the layer (ii) comes into contact with the cells, or the coat layer includes the layer (i) and the layer (i) is disposed at a position at which the layer (i) comes into contact with the cells, and the culture medium includes the cell adhesion factor.

Abstract: A biogenic substance detector with high reaction efficiency and detection sensitivity is obtained. The biogenic substance detector includes: a plurality of chambers 1011 for hybridizing a target and a probe; and a passage 1012 provided between the chambers 1011. The cross-sectional area of the passage 1012 taken perpendicularly to the chamber-passage alignment direction is smaller than the cross-sectional area of each chamber 1011 taken perpendicularly to the chamber-passage alignment direction, and a probe-fixing area 1013 is provided over the entire inside wall of each chamber 1011. One kind of probe is fixed in one chamber 1011. In the hybridization step, the sample solution is made to move back and forth within the chambers 1011 and the passage 1012 using a pump 102.

Abstract: A biological substance detection cartridge, including: a reaction vessel for reacting a probe with a specific biological substance included in a sample solution, the reaction vessel having a region for fixing the probe for detecting the biological substance; a porous membrane facing the inside of the reaction vessel; a gas-liquid separation membrane superposed with the porous membrane; and a air discharge component which is provided on the opposite side of the gas-liquid separation membrane from the side contacting the porous membrane, and with which the interior can be kept at negative pressure during the reaction between the biological substance and the probe.

Abstract: A biogenic substance detector with high reaction efficiency and detection sensitivity is obtained. The biogenic substance detector includes: a plurality of chambers 1011 for hybridizing a target and a probe; and a passage 1012 provided between the chambers 1011. The cross-sectional area of the passage 1012 taken perpendicularly to the chamber-passage alignment direction is smaller than the cross-sectional area of each chamber 1011 taken perpendicularly to the chamber-passage alignment direction, and a probe-fixing area 1013 is provided over the entire inside wall of each chamber 1011. One kind of probe is fixed in one chamber 1011. In the hybridization step, the sample solution is made to move back and forth within the chambers 1011 and the passage 1012 using a pump 102.