Abstract: Methods including (1) a step of obtaining a water dispersion of a pea ground product by dry pulverizing the pea and mixing same with water, or wet pulverizing the pea in water, (2) a step of obtaining an enzymatic reaction mixture by adding transglutaminase to a solution obtained by removing solid substances from the water dispersion obtained in step (1) or the water dispersion obtained in step (1), and (3) a step of adding an acid to the enzymatic reaction mixture obtained in (2) and collecting the precipitate, are useful for preparing a modified pea protein having increased viscosity.

Abstract: Methods including performing (I) a step of adding an acid to a water dispersion of a pea ground product and collecting the precipitate, and (II) a step of conducting an enzyme reaction by adding protein deamidase, in this order or reverse order, are useful for improving the solubility of a modified pea protein.

Abstract: A male fastening tool body includes a body part, a flange part which has a seat surface, an annular concave part formed between the body part and the seat surface, and a resinous ring-shaped member disposed at the annular concave part. The annular concave part has an annular concave peripheral edge portion. The resinous ring-shaped member has an annular projected part which fills almost the entire annular concave part and a lower surface of which is projected downward beyond the seat surface and an annular peripheral part, having a tapered bottom surface or a curved bottom surface, which is formed between the annular projected part and the outer edge of the resinous ring-shaped member and becomes gradually thinner, with an outer edge of the resinous ring-shaped member being located at an outer edge of the annular concave peripheral edge portion or slightly toward the side of the body part.

Abstract: A bolt has a bolt body including a screw part, a head part formed at an upper end of the screw part, and a flange part formed at a lower end of the head part; and an unconductive ring-shaped resinous member covering a peripheral portion of the flange part. The flange part has a disk-shaped flange body portion; and a seat surface-forming portion having a seat surface which is pressed against a portion where the bolt is to be mounted. The ring-shaped member has an edge part which is located at a position nearer to a lower end of the screw part than the seat surface and outward from the flange body portion and an annular skirt part whose diameter gradually increases to the edge part. The annular skirt part is so formed that it deforms when the bolt is mounted on the portion where the bolt is to be mounted and forms an annular liquid-tight sealing portion for the portion where the bolt is to be mounted.

Abstract: The present invention provides an enzyme preparation containing, as the active ingredient, an acidic or alkaline substance capable of shifting the pH value of the preparation toward a pH range wherein the expression of activity of a transglutaminase is inhibited when the transglutaminase is dissolved in the presence of collagen in a solution. The present invention also provides a process for producing a food by using the aforementioned enzyme preparation.

Abstract: A male fastening tool body includes a body part, a flange part which has a seat surface, an annular concave part formed between the body part and the seat surface, and a resinous ring-shaped member disposed at the annular concave part. The annular concave part has an annular concave peripheral edge portion. The resinous ring-shaped member has an annular projected part which fills almost the entire annular concave part and a lower surface of which is projected downward beyond the seat surface and an annular peripheral part, having a tapered bottom surface or a curved bottom surface, which is formed between the annular projected part and the outer edge of the resinous ring-shaped member and becomes gradually thinner, with an outer edge of the resinous ring-shaped member being located at an outer edge of the annular concave peripheral edge portion or slightly toward the side of the body part.

Abstract: By using transglutaminase, collagen, and calcium chloride or magnesium chloride as the active ingredients, raw food materials such as meat pieces can be sufficiently adhesion-molded.

Abstract: The invention provides a method for measuring the protease activity of a transglutaminase-containing product comprising: mixing an aqueous solution (sample solution) of a transglutaminase-containing product and an aqueous solution of dimethylcasein in a prescribed ratio of transglutaminase activity to quantity of dimethylcasein, allowing the mixture to stand under prescribed conditions to cause a degradation reaction by protease to progress, adding an acid, filtering, and measuring the concentration of the protein in the filtrate. The invention is particularly useful for transglutaminase formulation for binding application and transglutaminase formulation for Surimi product.

Abstract: A nut 1 of the present invention has a nut body 2 including a female screw part 5 extended axially and a flange part 4 projected outward from a side surface of the nut body 2; and an unconductive ring-shaped resinous member 3 covering a peripheral portion of the flange part 4. The flange part 4 has a disk-shaped flange body portion 41; and a seat surface-forming portion 42 which is extended downward from a lower surface of the flange body portion 41, has a smaller diameter than the flange body portion 41, and has a seat surface 43 to be pressed against a portion where the nut is to be mounted. The unconductive ring-shaped resinous member 3 has an edge part 31 located below the seat surface 43 and outward from the flange body portion 4; and an annular skirt part 32 whose diameter gradually increases to the edge part 31.

Abstract: A bolt has a bolt body including a screw part, a head part formed at an upper end of the screw part, and a flange part formed at a lower end of the head part; and an unconductive ring-shaped resinous member covering a peripheral portion of the flange part. The flange part has a disk-shaped flange body portion; and a seat surface-forming portion having a seat surface which is pressed against a portion where the bolt is to be mounted. The ring-shaped member has an edge part which is located at a position nearer to a lower end of the screw part than the seat surface and outward from the flange body portion and an annular skirt part whose diameter gradually increases to the edge part. The annular skirt part is so formed that it deforms when the bolt is mounted on the portion where the bolt is to be mounted and forms an annular liquid-tight sealing portion for the portion where the bolt is to be mounted.

Abstract: The invention provides a treatment method capable of reducing the protease present as an impurity in transglutaminase-containing products without reducing the activity of transglutaminase; a method for manufacturing transglutaminase-containing products with reduced protease activity by a treatment comprising maintaining a transglutaminase-containing product in which protease is present for 10 minutes or more but not more than 60 hours at greater than pH 9.0 but less than pH 13.0 at a temperature of 0° C. or greater but less than 50° C.; a transglutaminase-containing product obtained by this treatment method; a transglutaminase formulation in which this transglutaminase-containing product is blended, and a method for manufacturing a food by adding this transglutaminase formulation.

Abstract: The invention provides a method for measuring the protease activity of a transglutaminase-containing product comprising: mixing an aqueous solution (sample solution) of a transglutaminase-containing product and an aqueous solution of dimethylcasein in a prescribed ratio of transglutaminase activity to quantity of dimethylcasein, allowing the mixture to stand under prescribed conditions to cause a degradation reaction by protease to progress, adding an acid, filtering, and measuring the concentration of the protein in the filtrate. The invention is particularly useful for transglutaminase formulation for binding application and transglutaninase formulation for Surimi product.

Abstract: The present invention provides an enzyme preparation containing, as the active ingredient, an acidic or alkaline substance capable of shifting the pH value of the preparation toward a pH range wherein the expression of activity of a transglutaminase is inhibited when the transglutaminase is dissolved in the presence of collagen in a solution. The present invention also provides a process for producing a food by using the aforementioned enzyme preparation.