Abstract: An enzyme electrode comprising an anode, a cathode, an immobilized enzyme membrane, and an electrolyte, which is characterized in that said membrane consists of a dense skin layer having selective hydrogen peroxide permeability and a porous layer wherein the enzyme is immobilized onto the porous layer of the membrane and said immobilized enzyme membrane is arranged in the electrode so that the skin layer is faced to the electrode and the porous layer immobilized with the enzyme is in the position of contacting with the test solution. The enzyme electrode is used for the polarographic determination of a slight amount of components in body fluid, body tissue, foodstuffs, or the like without any undesirable hindrance with low molecular interfering materials without using any other specific means for removing such low molecular interfering materials.