Patents by Inventor Robert A. Eickholt
Robert A. Eickholt has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10703971Abstract: Alkaline phosphatase chemiluminescent substrate formulations are provided exhibiting rapid incubation periods and improved stability for use in immunoassays.Type: GrantFiled: June 30, 2017Date of Patent: July 7, 2020Assignee: Beckman Coulter, Inc.Inventors: Renuka De Silva, Robert A. Eickholt, Jaya S. Koti, Mark D. Sandison, David L. Schumm, Wenhua Xie
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Publication number: 20190161675Abstract: Alkaline phosphatase chemiluminescent substrate formulations are provided exhibiting rapid incubation periods and improved stability for use in immunoassays.Type: ApplicationFiled: June 30, 2017Publication date: May 30, 2019Applicant: Beckman Coulter, Inc.Inventors: Renuka DE SILVA, Robert A. EICKHOLT, Jaya S. KOTI, Mark D. SANDISON, David L. SCHUMM, Wenhua XIE
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Patent number: 8772501Abstract: The present invention provides fluorescent dyes that are based on firefly luciferin structure. These dyes are optimally excited at shorter wavelengths and have Stokes shift of at least 50 nm. The fluorescent dyes of the invention are useful for preparation of dye-conjugates, which can be used in detection of an analyte in a sample.Type: GrantFiled: January 24, 2013Date of Patent: July 8, 2014Assignee: Beckman Coulter, Inc.Inventors: Hashem Akhavan-Tafti, Renuka De Silva, Guoping Wang, Robert A. Eickholt, Ravinder Gupta, Lakshmi S. Kaanumalle
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Patent number: 8367842Abstract: The present invention provides fluorescent dyes that are based on firefly luciferin structure. These dyes are optimally excited at shorter wavelengths and have Stokes shift of at least 50 nm. The fluorescent dyes of the invention are useful for preparation of dye-conjugates, which can be used in detection of an analyte in a sample.Type: GrantFiled: July 15, 2010Date of Patent: February 5, 2013Assignee: Beckman Coulter, Inc.Inventors: Hashem Akhavan-Tafti, Renuka De Silva, Guoping Wang, Robert A. Eickholt, Ravinder K. Gupta, Lakshmi S. Kaanumalle
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Patent number: 8137990Abstract: Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-O?is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.Type: GrantFiled: August 14, 2009Date of Patent: March 20, 2012Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Kenneth S. Lauwers, Richard S. Handley
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Publication number: 20110014599Abstract: The present invention provides fluorescent dyes that are based on firefly luciferin structure. These dyes are optimally excited at shorter wavelengths and have Stokes shift of at least 50 nm. The fluorescent dyes of the invention are useful for preparation of dye-conjugates, which can be used in detection of an analyte in a sample.Type: ApplicationFiled: July 15, 2010Publication date: January 20, 2011Applicant: BECKMAN COULTER, INC.Inventors: Hashem AKHAVAN-TAFTI, Renuka DE SlLVA, Guoping WANG, Robert A. EICKHOLT, Ravinder K. GUPTA, Lakshmi S. KAANUMALLE
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Patent number: 7704752Abstract: Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-O? is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.Type: GrantFiled: June 23, 2008Date of Patent: April 27, 2010Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Kenneth S. Lauwers, Richard S. Handley
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Publication number: 20100055800Abstract: Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-O?is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.Type: ApplicationFiled: August 14, 2009Publication date: March 4, 2010Applicant: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Kenneth S. Lauwers, Richard S. Handley
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Publication number: 20080261318Abstract: Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-O?is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.Type: ApplicationFiled: June 23, 2008Publication date: October 23, 2008Applicant: LUMIGEN, INC.Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Kenneth S. Lauwers, Richard S. Handley
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Patent number: 7390670Abstract: Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig—OH or Sig—O? is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.Type: GrantFiled: February 20, 2003Date of Patent: June 24, 2008Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Kenneth S. Lauwers, Richard S. Handley
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Publication number: 20070190526Abstract: Methods and materials are disclosed for rapid and simple extraction and isolation of nucleic acids, particularly RNA, from a biological sample involving the use of an alkaline reagent followed by an acidic solution and a solid phase binding material that has the ability to liberate nucleic acids from biological samples, including whole blood, without first performing any preliminary lysis to disrupt cells or viruses. No detergents or chaotropic substances for lysing cells or viruses are needed or used. Viral, bacterial and mammalian genomic RNA can be obtained using the method of the invention. RNA obtained by the present method is suitable for use in downstream processes such as RT-PCR.Type: ApplicationFiled: February 15, 2007Publication date: August 16, 2007Inventors: Hashem Akhavan-Tafti, Renuka de Silva, Robert A. Eickholt, Michael E. Mazelis, Wenhuas Xie, Richard S. Handley, Monica A. Bray, Michelle L. Mastronardi, Elizabeth A. O'Conner, Sarada Siripurapu
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Patent number: 6919463Abstract: Compounds useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is an aromatic or heteroaromatic ring group, B is a boron atom, and R5 and R6 are independently selected from hydrogen and lower alkyl groups and can be joined together as a straight or branched alkylene chain forming a five or six-membered ring. A detectable product compound of the formula Sig-OH Is produced and detected by measuring color, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The compounds can be used for detection in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.Type: GrantFiled: June 20, 2003Date of Patent: July 19, 2005Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Kenneth S. Lauwers, Richard S. Handley
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Publication number: 20050106577Abstract: Solid phase materials for binding nucleic acids and methods of their use are disclosed. The materials feature a cleavable linker portion which can be cleaved to release bound nucleic acids. The solid phase materials comprise a solid support portion comprising a matrix selected from silica, glass, insoluble synthetic polymers, and insoluble polysaccharides to which is attached a nucleic acid binding portion for attracting and binding nucleic acids, the nucleic acid binding portion (NAB) being linked by a cleavable linker portion to the solid support portion. Preferred nucleic acid binding portions comprise a ternary or quaternary onium group. The materials can be in the form of microparticles, fibers, beads, membranes, test tubes or microwells and can further comprise a magnetic core portion. Methods of binding nucleic acids using the cleavable solid supports are disclosed as are their use in methods of isolating or purifying nucleic acids.Type: ApplicationFiled: November 17, 2003Publication date: May 19, 2005Inventors: Hashem Akhavan-Tafti, Renuka Silva, Robert Eickholt, C. Gundlach, Richard Handley, Kenneth Lauwers, Mark Sandison, Wenhua Xie
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Publication number: 20040171098Abstract: Compounds useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: 1Type: ApplicationFiled: June 20, 2003Publication date: September 2, 2004Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Kenneth S. Lauwers, Richard S. Handley
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Publication number: 20040166539Abstract: Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: 1Type: ApplicationFiled: February 20, 2003Publication date: August 26, 2004Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Kenneth S. Lauwers, Richard S. Handley
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Patent number: 6410732Abstract: Synthetic processes and intermediates are disclosed for the preparation of N-arylacridancarboxylic acid derivatives. The derivatives are esters, thioesters, amides and sulfonimides. A key feature of the processes is the preparation of N-aryl substituted intermediates by formation of a bond between the nitrogen atom of the acridan ring and a carbon atom of another aromatic or heteroaromatic ring compound. The arylation reaction is catalyzed by a palladium catalyst. The N-arylacridancarboxylic acid derivatives are useful in methods for producing light and in assays for peroxidase enzymes and enzyme inhibitors and in assays employing enzyme-labeled specific binding pairs.Type: GrantFiled: January 25, 2001Date of Patent: June 25, 2002Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Richard S. Handley
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Publication number: 20010031869Abstract: Synthetic processes and intermediates are disclosed for the preparation of N-arylacridancarboxylic acid derivatives. The derivatives are esters, thioesters, amides and sulfonimides. A key feature of the processes is the preparation of N-aryl substituted intermendiates by formation of a bond between the nitrogen atom of the acridan ring and a carbon atom of another aromatic or heteroaromatic ring compound. The arylation reaction is catalyzed by a palladium catalyst. The N-arylacridancarboxylic acid derivatives are useful in methods for producing light and in assays for peroxidase enzymes and enzyme inhibitors and in assays employing enzyme-labeled specific binding pairs.Type: ApplicationFiled: January 25, 2001Publication date: October 18, 2001Inventors: Hashem Akhavan-Tafti, Robert A. Eickholt, Richard S. Handley
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Patent number: 5936101Abstract: A process for producing a stable triggerable dioxetane comprising;(a) reacting a vinyl sulfide compound containing a sulfur-substituent SR.sub.4, wherein R.sub.4 is an organic group containing 1 to 20 carbon atoms and optionally heteroatoms, with oxygen and light in the presence of a photosensitizer to form an intermediate sulfur-substituted dioxetane compound; and(b) reacting the sulfur-substituted dioxetane compound with an electrophilic compound E--Y and a hydroxylic compound R.sub.5 OH selected from the group consisting of alcohols, phenols and carboxylic acids or their salts and containing an OR.sub.5 group to replace the SR.sub.4 group of the dioxetane with the OR.sub.5 group.Type: GrantFiled: December 22, 1997Date of Patent: August 10, 1999Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Zahra Arghavani, Robert A. Eickholt
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Patent number: 5883287Abstract: Vinyl sulfides bearing protected arlyoxy substituents and a process for their preparation are provided. In the process a vinyl sulfide is formed by coupling a ketone compound and a thioester compound with a low valent titanium reagent to form both carbon-carbon bonds in the same step. The vinyl sulfide compounds are useful as intermediates which are convertible into triggerable sulfur-substituted dioxetanes by addition of oxygen to the double bond. The sulfur-substituted dioxetanes additionally are useful for producing chemiluminescence or for further conversion into triggerable alkoxy, alkenyloxy, alkynloxy, aryloxy, aralkyloxy, or acyloxy-substituted dioxetanes. Triggerable dioxetanes are useful for producing chemiluminescence and in detecting activating agents in assays such as immunoassays and nucleic acid hybridization assays.Type: GrantFiled: December 22, 1997Date of Patent: March 16, 1999Assignee: Lumigen, Inc.Inventors: Hashem Akhavan-Tafti, Zahra Arghavani, Robert A. Eickholt
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Patent number: 5773628Abstract: A chemiluminescent assay method and compositions are described which use a haloalkoxy group-substituted dioxetane which is deprotected by a hydrolytic enzyme to undergo a chemiluminescent reaction. Chemiluminescent 1,2-dioxetane compounds substituted on the dioxetane ring with a haloalkoxy group which can be triggered by a reagent to generate light are disclosed. Haloalkoxy group-substituted dioxetanes are useful for the detection of triggering agents including enzymes. The enzyme may be present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the enzyme is bound to a reporter molecule.Type: GrantFiled: November 14, 1994Date of Patent: June 30, 1998Assignee: Tropix, Inc.Inventors: Hashem Akhavan-Tafti, Zahra Arghavani, Robert A. Eickholt, Khaledur S. Rashid