Patents by Inventor Robert A. Setterquist
Robert A. Setterquist has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20210190809Abstract: Purifying target biomolecules, such as nucleic acids or proteins, from a biological source is a time intensive process and is typically performed by a skilled technician or scientist owing to the highly technical nature of the work. Systems, devices, and methods disclosed herein enable the automated bioprocessing and purification of target biomolecules from a biological source. For example, an instrument and disposable cartridge are provided for automatedly isolating and purifying nucleic acids (such as plasmid DNA from a bacterial culture) or for isolating protein from any biological sample. Such an exemplary instrument and cartridge can work in concert to timely release, mix, and move the target biomolecule and various reagents and buffers through a target biomolecule purification process, resulting in a purified target biomolecule with less manual oversight than traditional approaches.Type: ApplicationFiled: December 15, 2020Publication date: June 24, 2021Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Alexander VLASSOV, Timothy BARTA, Justin INSLEE, Ethan GENTERT, Robert SMITH, Sara THIELE, Carolyn ADAMS, Kraig KRUGER, Robert Schneider, Wade LUKIANOW, Jay GRECO, Jason JONES, David CERRONE, Sumonto GHOSH, Brian VAJDA, Andrew BASTA, Allan EVANS, Christopher CROWLEY, Robert SETTERQUIST, Anne Cox, Mark MAYERNICK
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Patent number: 9464315Abstract: The invention relates to methods of separating polynucleotides, such as DNA, RNA and PNA, from solutions containing polynucleotides by reversibly binding the polynucleotides to a solid surface, such as a magnetic microparticle.Type: GrantFiled: March 25, 2013Date of Patent: October 11, 2016Assignee: Applied Biosystems, LLCInventors: Gary Latham, Xingwang Fang, Richard Conrad, Jon Kemppainen, Robert Setterquist, Brittan Pasloske
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Publication number: 20160160194Abstract: The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.Type: ApplicationFiled: December 22, 2014Publication date: June 9, 2016Inventors: LIANGJING CHEN, ROBERT SETTERQUIST, GARY LATHAM
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Patent number: 8956841Abstract: The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.Type: GrantFiled: December 28, 2012Date of Patent: February 17, 2015Assignee: Applied Biosystems, LLCInventors: Liangjing Chen, Robert Setterquist, Gary Latham
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Publication number: 20150031584Abstract: The present invention provides novel algorithms for designing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts. Such oligonucleotides are particularly useful as primers for reverse transcription. The invention also provides compositions containing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts.Type: ApplicationFiled: July 10, 2014Publication date: January 29, 2015Inventors: Xiaowei WANG, Xiaohui Wang, Robert Setterquist
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Patent number: 8809513Abstract: The present invention provides novel algorithms for designing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts. Such oligonucleotides are particularly useful as primers for reverse transcription. The invention also provides compositions containing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts.Type: GrantFiled: August 22, 2012Date of Patent: August 19, 2014Assignee: Applied Biosystems, LLCInventors: Xiaowei Wang, Xiaohui Wang, Robert Setterquist
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Publication number: 20130302810Abstract: The invention relates to methods of separating polynucleotides, such as DNA, RNA and PNA, from solutions containing polynucleotides by reversibly binding the polynucleotides to a solid surface, such as a magnetic microparticle.Type: ApplicationFiled: March 25, 2013Publication date: November 14, 2013Inventors: Gary LATHAM, Xingwang Fang, Richard Conrad, Jon Kemppainen, Robert Setterquist, Brittan Pasloske
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Patent number: 8426126Abstract: The invention relates to methods of separating polynucleotides, such as DNA, RNA and PNA, from solutions containing polynucleotides by reversibly binding the polynucleotides to a solid surface, such as a magnetic microparticle.Type: GrantFiled: September 30, 2004Date of Patent: April 23, 2013Assignee: Applied Biosystems, LLCInventors: Gary J. Latham, Xingwang Fang, Richard C. Conrad, Jon A. Kemppainen, Robert A. Setterquist, Brittan L. Pasloske
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Publication number: 20130059759Abstract: The present invention provides novel algorithms for designing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts. Such oligonucleotides are particularly useful as primers for reverse transcription. The invention also provides compositions containing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts.Type: ApplicationFiled: August 22, 2012Publication date: March 7, 2013Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Xiaowei Wang, Xiaohui Wang, Robert Setterquist
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Patent number: 8361754Abstract: The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.Type: GrantFiled: September 28, 2009Date of Patent: January 29, 2013Assignee: Applied Biosystems, LLCInventors: Liangjing Chen, Robert A. Setterquist, Gary Latham
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Patent number: 8268987Abstract: The present invention provides novel algorithms for designing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts. Such oligonucleotides are particularly useful as primers for reverse transcription. The invention also provides compositions containing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts.Type: GrantFiled: November 19, 2010Date of Patent: September 18, 2012Assignee: Applied Biosystems, LLCInventors: Robert Setterquist, Xiaohui Wang, Xiaowei Wang
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Publication number: 20110301343Abstract: The present invention concerns a system for isolating, depleting, and/or preventing the amplification of a targeted nucleic acid, such as mRNA or rRNA, from a sample comprising targeted and nontargeted nucleic acids.Type: ApplicationFiled: March 14, 2011Publication date: December 8, 2011Applicant: Life Technologies CorporationInventors: Leopoldo MENDOZA, Sharmili Moturi, Robert Setterquist, John Penn Whitley
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Publication number: 20110257039Abstract: The present invention provides novel algorithms for designing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts. Such oligonucleotides are particularly useful as primers for reverse transcription. The invention also provides compositions containing oligonucleotides that do not substantially hybridize to a small group of unwanted transcripts, while hybridizing to most other transcripts.Type: ApplicationFiled: November 19, 2010Publication date: October 20, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Xiaowei Wang, Xiaohui Wang, Robert Setterquist
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Publication number: 20100203597Abstract: The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.Type: ApplicationFiled: September 28, 2009Publication date: August 12, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Liangjing Chen, Robert A. Setterquist, Gary Latham
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Publication number: 20090264635Abstract: The present invention concerns a system for isolating, depleting, and/or preventing the amplification of a targeted nucleic acid, such as mRNA or rRNA, from a sample comprising targeted and nontargeted nucleic acids.Type: ApplicationFiled: March 27, 2006Publication date: October 22, 2009Applicant: APPLERA CORPORATIONInventors: Leopoldo Mendoza, Sharmili Moturi, JR., Robert Setterquist, John Penn Whitley
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Patent number: 7595179Abstract: The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.Type: GrantFiled: April 19, 2004Date of Patent: September 29, 2009Assignee: Applied Biosystems, LLCInventors: Liangjing Chen, Robert A. Setterquist, Gary J. Latham
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Publication number: 20060257902Abstract: The present invention concerns a system for isolating, depleting, and/or preventing the amplification of a targeted nucleic acid, such as mRNA or rRNA, from a sample comprising targeted and nontargeted nucleic acids.Type: ApplicationFiled: March 27, 2006Publication date: November 16, 2006Inventors: Leopold Mendoza, Sharmili Moturi, Robert Setterquist, John Whitley
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Patent number: 6994963Abstract: This invention pertains to a method for generating a pool of nucleic acid fragments useful for in vitro recombination and the creation of novel DNA sequences that encode desirable proteins or enzymes. The invention provides a defined mixture of nucleic acids and methods for use in the synthesis, mutagenesis, and recombination of nucleic acids. Nucleic acids may be synthesized by creating a nucleic acid extension ladder, annealing the extension ladder to template nucleic acids, and further extending the ladder of nucleic acids. The invention also relates to methods for performing repeated cycles of synthesis for the purpose of mutagenesis or recombination, methods for producing mutant peptides and proteins from the mutagenized or recombined nucleic acids, and methods for selecting a peptide, polypeptide or protein having altered biological activities.Type: GrantFiled: July 10, 2000Date of Patent: February 7, 2006Assignee: Ambion, Inc.Inventors: George L. Murphy, Robert A. Setterquist, Andrew D. Ellington
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Publication number: 20050232934Abstract: The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.Type: ApplicationFiled: April 19, 2004Publication date: October 20, 2005Applicant: Ambion, Inc.Inventors: Liangjing Chen, Robert Setterquist, Gary Latham
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Publication number: 20050208510Abstract: The invention relates to methods of separating polynucleotides, such as DNA, RNA and PNA, from solutions containing polynucleotides by reversibly binding the polynucleotides to a solid surface, such as a magnetic microparticle.Type: ApplicationFiled: September 30, 2004Publication date: September 22, 2005Inventors: Gary Latham, Xingwang Fang, Richard Conrad, Jon Kemppainen, Robert Setterquist, Brittan Pasloske