Abstract: Methods and compositions for performing nucleic acid duplication and amplification reactions are provided. A single-stranded nucleic acid binding protein is selected and provided in the reaction mixture which is assembled at a low, nonstringent temperature to include all of the necessary reagents for successful nucleic acid duplication or amplification reactions. By incorporating a single-stranded nucleic acid binding protein into the reaction mixture at low temperature, the generation of nonspecific products such as amplification products is improved despite the reaction mixture having been fully assembled at a nonstringent temperature.

Abstract: Methods and compositions for performing nucleic acid duplication and amplification reactions are provided. A single-stranded nucleic acid binding protein is selected and provided in the reaction mixture which is assembled at a low, nonstringent temperature to include all of the necessary reagents for successful nucleic acid duplication or amplification reactions. By incorporating a single-stranded nucleic acid binding protein into the reaction mixture at low temperature, the generation of nonspecific products such as amplification products is improved despite the reaction mixture having been fully assembled at a nonstringent temperature.

Abstract: Methods and compositions for performing nucleic acid duplication and amplification reactions are provided. A single-stranded nucleic acid binding protein is selected and provided in the reaction mixture which is assembled at a low, nonstringent temperature to include all of the necessary reagents for successful nucleic acid duplication or amplification reactions. By incorporating a single-stranded nucleic acid binding protein into the reaction mixture at low temperature, the generation of nonspecific products such as amplification products is improved despite the reaction mixture having been fully assembled at a nonstringent temperature.

Abstract: A composition containing a nuclease, preferably Exonuclease I, and a phosphatase, preferably Shrimp Alkaline Phosphatase, wherein the enzymes are combined in a single composition yet each enzyme retains significant functional activity over time. Combining Exonuclease I and Shrimp Alkaline Phosphatase into one composition allows simplified processing of amplified DNA to degrade residual primers and nucleotide triphosphates thereby facilitating subsequent DNA analysis.

Abstract: A composition containing a nuclease, preferably Exonuclease I, and a phosphatase, preferably Shrimp Alkaline Phosphatase, wherein the enzymes are combined in a single composition yet each enzyme retains significant functional activity over time. Combining Exonuclease I and Shrimp Alkaline Phosphatase into one composition allows simplified processing of amplified DNA to degrade residual primers and nucleotide triphosphates thereby facilitating subsequent DNA analysis.

Abstract: A composition containing a nuclease, preferably Exonuclease I, and a phosphatase, preferably Shrimp Alkaline Phosphatase, wherein the enzymes are combined in a single composition yet each enzyme retains significant functional activity over time. Combining Exonuclease I and Shrimp Alkaline Phosphatase into one composition allows simplified processing of amplified DNA to degrade residual primers and nucleotide triphosphates thereby facilitating subsequent DNA analysis.

Abstract: A composition containing a nuclease, preferably Exonuclease I, and a phosphatase, preferably Shrimp Alkaline Phosphatase, wherein the enzymes are combined in a single composition yet each enzyme retains significant functional activity over time. Combining Exonuclease I and Shrimp Alkaline Phosphatase into one composition allows simplified processing of amplified DNA to degrade residual primers and nucleotide triphosphates thereby facilitating subsequent DNA analysis.

Abstract: A method of making a nonmagnetic, austenitic, manganese-chromium-nickel-nitrogen stainless steel uses intermediate addition of ferroalloys and novel mixtures of oxygen and nitrogen to maintain the temperature of the melt below about 3150.degree. F. (1732.degree. C.). The method improves on the economics of making specialty steels by reducing the cost of raw materials and refractories.

Abstract: Compounds of the formula IV ##STR1## wherein R is hydrogen or alkyl of 1 or 2 carbon atoms; wherein R.sub.1 is alkyl of 1 to 3 carbon atoms, inclusive; or ##STR2## is a 2-cycloalkenylene ring of 5 or 6 carbon atoms; wherein R.sub.2 is hydrogen, methyl or ethyl; wherein R.sub.3 is hydrogen, fluoro, chloro, bromo, nitro and --CF.sub.3 ; and wherein Ar is phenyl, o-chlorophenyl, o-fluorophenyl, 2,6-difluorophenyl, and 2-pyridyl, are prepared by treating a hydrazino compound of the formula: ##STR3## wherein Ar and R.sub.3 are defined as above, with a dicarbonyl compound II: ##STR4## and cyclizing the resulting compound III to give the compound IV above. Compound IV, including the pharmacologically acceptable acid addition salt of these compounds, have sedative, anxiolytic and muscle-relaxing activity and can be used for the treatment of anxieties or muscle strains of mammals, including man.

Abstract: Compounds of the formula V ##STR1## wherein R.sub.1 is hydrogen, alkyl of 1 to 3 carbon atoms, inclusive; ##STR2## in which n is 2 or 3; and X is hydrogen, fluoro or chloro; wherein R.sub.2 is hydrogen, methyl or ethyl; wherein R.sub.3 is hydrogen, fluoro, chloro, bromo, trifluoromethyl and nitro; and wherein Ar is phenyl, o-chlorophenyl, o-fluorophenyl, 2,6-difluorophenyl or 2-pyridyl are prepared by treating a hydrazino compound of the formula: ##STR3## WHEREIN Ar, R.sub.2 and R.sub.3 are defined as above with an alkyl oxalyl chloride and cyclizing the obtained products. Compound V, including the pharmacologically acceptable acid addition salt thereof, have sedative, anxiolytic and muscle-relaxing activity and can be used for the treatment of anxieties or muscle strains of mammals, including man.

Abstract: Compounds of the formula IV: ##STR1## wherein R.sub.o is hydrogen, alkyl of 1 to 3 carbon atoms, inclusive, --C.sub.n H.sub.2n OR in which n is an integer of 1 to 3, inclusive, and R is hydrogen or methyl, ##STR2## in which n is defined as above, and R.sub.6 and R.sub.7 are hydrogen or alkyl as defined above, or together ##STR3## is pyrrolidino, piperidino, morpholino or ##STR4## in which R.sub.8 is methyl, ethyl, or 2-hydroxyethyl, ##STR5## in which m is an integer of 0 to 3, inclusive, and R.sub.9 is hydrogen, lower alkyl of from 1 to 3 carbon atoms, inclusive, wherein R.sub.1 is hydrogen or methyl; wherein R.sub.2 is phenyl, o-chlorophenyl, o-fluorophenyl, 2,6-difluorophenyl, or 2-pyridyl; and wherein R.sub.3 is hydrogen, fluoro, chloro, bromo, trifluoromethyl, or nitro.