Abstract: Methods for determining the presence of an analyte in a sample are disclosed, in which a capture agent is bound to the analyte at a first epitope and a detection agent is bound at a second epitope, and in which the detection agent includes an oligonucleotide to which a labeled, complementary oligonucleotide can be hybridized.

Abstract: The present invention relates to methods for lyophilizing eukaryotic cells and isolating intact nucleic acids from such cells, and related kits for using the same.

Abstract: The present invention relates to methods for lyophilizing eukaryotic cells and isolating intact nucleic acids from such cells, and related kits for using the same.

Abstract: The generation and extensive synthesis (“amplification”) of a cDNA sequence of interest is attained through a linked series of multi-cycle primer extension reactions (RT-LLA). The primers used in each of the primer extension reactions of the process contain non-replicable and, in some cases non-replicable and cleavable, elements that halt nucleic acid synthesis and thereby prevent the synthesized molecules from serving as templates in subsequent cycles. Synthesized molecules accumulate during primer extension in a mathematically linear fashion, thereby rendering the process relatively insensitive to contaminating nucleic acids. Multiple primer sets are employed, and simultaneously included in the reaction mixture, thereby ensuring the accumulation of a large number of copies of the cDNA sequence of interest.

Abstract: A hinge for mounting a door to an enclosure includes a first leaf, a second leaf, and a pin. The first leaf includes a first tongue attached to a surface formed on one of the door or the enclosure, and a first knuckle depending from the first tongue. The first knuckle includes a tab depending therefrom, and the tab extends through an aperture in a flange depending from the surface. The second leaf includes a second tongue coupled to another of the door or the enclosure, and a second knuckle coupled to the second tongue. The pin extends through the first and second knuckles. A method of assembling a hinge to a door includes: extending a portion of the hinge within an aperture in the door; applying a magnetic force to the portion of the hinge to secure the portion of the hinge within the aperture; and securing the hinge to the door with a fastener while applying the magnetic force.

Abstract: The present invention relates to methods for lyophilizing eukaryotic cells and isolating intact nucleic acids from such cells, and related kits for using the same.

Abstract: A hinge for mounting a door to an enclosure includes a first leaf, a second leaf, and a pin. The first leaf includes a first tongue attached to a surface formed on one of the door or the enclosure, and a first knuckle depending from the first tongue. The first knuckle includes a tab depending therefrom, and the tab extends through an aperture in a flange depending from the surface. The second leaf includes a second tongue coupled to another of the door or the enclosure, and a second knuckle coupled to the second tongue. The pin extends through the first and second knuckles. A method of assembling a hinge to a door includes: extending a portion of the hinge within an aperture in the door; applying a magnetic force to the portion of the hinge to secure the portion of the hinge within the aperture; and securing the hinge to the door with a fastener while applying the magnetic force.

Abstract: The present invention relates to methods for lyophilizing eukaryotic cells and isolating intact nucleic acids from such cells, and related kits for using the same.

Abstract: A fastener designed to press into two slots of a barrier and become captivated with spring tension barbs is disclosed. The fastener thus allows two vertical barriers to be fastened together by driving a screw through a first barrier, a second barrier, and then the fastener, to compress the barriers in place during assembly. The fastener may be provided with a nut holding cage with caged nut or a pair of thread engaging flanges to secure the screw to the fastener.

Abstract: A door hinge assembly for use in an electrical appliance cabinet includes a door hinge member having a door plate attached to one side of an elbow, a hinge pin sleeve attached to the opposite side of the elbow, and a flange extending upwardly from the elbow. A hinge pin is removably engageable with the hinge pin sleeve, which is further removably engageable with a top edge on the flange.

Abstract: A door hinge assembly for use in an electrical appliance cabinet includes a door hinge member having a door plate attached to one side of an elbow, a hinge pin sleeve attached to the opposite side of the elbow, and a flange extending upwardly from the elbow. A hinge pin is removably engageable with the hinge pin sleeve, which is further removably engageable with a top edge on the flange.

Abstract: The present invention relates to methods for lyophilizing eukaryotic cells and isolating intact nucleic acids from such cells, and related kits for using the same.

Abstract: In an exemplary embodiment of the invention, a machine for shaping a bus bar comprises a first retaining device, a second retaining device, a twisting device and an offset mechanism. The first retaining device is arranged to secure a first section of the bus bar in a first plane. The second retaining device is arranged to secure a second section of the bus bar. The twisting device is connected to the second retaining device and rotates the second retaining device to twist the second section of the bus bar about a longitudinal axis of the bus bar positioning the bus bar in a second plane. The offset mechanism is connected to the first retaining device and is arranged to offset the second section of the bus bar in a third plane, the third plane generally parallel to the second plane.

Abstract: The extensive synthesis (“amplification”) of a nucleic acid sequence of interest is attained through a linked series of multi-cycle primer extension reactions (LLA). The primers used in each of the primer extension reactions of the process contain non-replicable and/or cleavable elements that halt nucleic acid synthesis and thereby prevent the synthesized molecules from serving as templates in subsequent cycles. Synthesized molecules accumulate during primer extension in a mathematically linear fashion, thereby rendering the process relatively insensitive to contaminating nucleic acids. Multiple primer sets are employed, and simultaneously included in the reaction mixture, thereby ensuring the accumulation of a large number of copies of the nucleic acid sequence of interest. The invention also provides for the detection of an amplified nucleic acid sequence of interest, as well as reagent kits for carrying out the reaction.

Abstract: The present invention relates to methods for lyophilizing eukaryotic cells and isolating intact nucleic acids from such cells, and related kits for using the same.

Abstract: The extensive synthesis ("amplification") of a nucleic acid sequence of interest is attained through a linked series of multi-cycle primer extension reactions (LLA). The primers used in each of the primer extension reactions of the process contain non-replicable elements that halt nucleic acid synthesis and thereby prevent the synthesized molecules from serving as templates in subsequent cycles. Synthesized molecules accumulate during primer extension in a mathematically linear fashion, thereby rendering the process relatively insensitive to contaminating nucleic acids. Multiple primer sets are employed, thereby ensuring the accumulation of a large number of copies of the nucleic acid sequence of interest. The invention also provides for the detection of an amplified nucleic acid sequence of interest, as well as reagent kits for carrying out the reaction.