Abstract: A method, system, and computer programming product for returning a response directly to a transaction request originator in a customer information control system (CICS) is provided. The method can include receiving a transaction request in a CICS and allocating resources in the CICS in response to receiving the transaction request. The method additionally can include determining whether the CICS is an intended endpoint of the transaction request. Finally, the method can include extracting an address of an originator of the transaction request from the transaction request and sending a response to the originator at the extracted address upon determining that the CICS is the intended endpoint of the transaction request, but otherwise routing the transaction request to a different CICS along with the address within the transaction request.

Abstract: A method of adjusting amplification curves in a PCR experiment includes receiving a plurality of amplification curves for a sample and computing a first parameter for each of the plurality of amplification curves. The method also includes computing a second parameter for each of the plurality of amplification curves and computing a third parameter using at least a portion of the first or second parameters. The method further includes computing an offset for each of the plurality of amplification curves. The offset is a function of the first parameter and the third parameter. Moreover, the method includes adjusting at least one of the plurality of amplification curves by subtracting the offset.

Abstract: A microfluidic device is described that has a slug mixing arrangement. A reaction well has an input flow channel with a first valve near the reaction well, and a second valve further from the reaction well. A fluid source is connected to the segment in between the two valves. A second fluid source is connected behind the second valve. The channel between the two valves receives the first fluid by blind filling when the two valves are closed. The reaction well receives the first fluid followed by the second fluid when the first and second valves are open.

Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

Abstract: New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available.

Abstract: A method for performing PCR on a liquid sample comprising a plurality of polynucleotide targets, wherein each polynucleotide target is present at very low concentration within the sample. The method comprises applying PCR reactants to the surface of a substrate to produce a plurality of reaction spots on the surface of the substrate; loading the liquid sample and a PCR reagent mixture onto the reaction spots; forming a sealed reaction chamber, having a volume of less than about 20 nanoliters, over each of the reaction spots; and amplifying the sample.

Abstract: A method for assigning runtime artifacts to software components. Source code and metadata are extracted from a library, where the metadata includes a component membership for the source code. A compile unit is formed from the extracted source code and metadata. Runtime artifacts are produced from the compile unit or an element derived from the compile unit. Each runtime artifact is interrogated to determine the defined component for each runtime artifact. The determined component is recorded.

Abstract: Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5? to the target sequence, and a second probe-binding sequence 3? to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, said first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.

Abstract: New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available.

Abstract: A method, system and computer program product for assigning runtime artifacts to software components. Source code and metadata are extracted from a library, where the metadata includes a component membership for the source code. A compile unit is formed from the extracted source code and metadata. Runtime artifacts are produced from the compile unit or an element derived from the compile unit. Each runtime artifact is interrogated to determine the defined component for each runtime artifact. The determined component is recorded.

Abstract: Methods and apparatus for tilting and filling a carton with a pouch and a flowable food product are described. The carton can be tilted prior to filling in order to minimize a void between the pouch and a sidewall of the carton. The carton is pivoted to a tilted orientation, such that the sidewall includes a lowermost inclined segment. The pouch is then inserted into the carton either prior to or after pivoting the carton to the tilted orientation so that the pouch can substantially rest against the inclined segment. The flowable food product is then at partially deposited into the carton, such that the flowable food product substantially fills in next to the pouch instead of between the pouch and the inclined segment. After a sufficient height of flowable food product has been deposited into the carton, the carton can be returned to a vertical orientation and closed.

Abstract: An SBS-formatted microfluidic device where the geometry of the plate defines an array of interrogation areas, and where each interrogation area encompasses at least one reaction site.

Abstract: An SBS-formatted microfluidic device where the geometry of the plate defines an array of interrogation areas, and where each interrogation area encompasses at least one reaction site.

Abstract: A modified atmosphere flow-wrap system and method and components thereof are provided for packaging a food product in a reduced oxygen atmosphere in a continuous or partially-continuous process. In one aspect, the flow-wrap system includes a saturation tunnel for saturating the food products with modified gas to reduce an amount of residual oxygen therein. In another aspect, the flow-wrap system includes a flow-wrapping station with a modified gas lance that extends into film webbing used to form the food product packages to insert modified gas into the film webbing and restrict oxygen from entering the food packages.

Abstract: A method for performing PCR on a liquid sample comprising a plurality of polynucleotide targets, wherein each polynucleotide target is present at very low concentration within the sample. The method comprises applying PCR reactants to the surface of a substrate to produce a plurality of reaction spots on the surface of the substrate; loading the liquid sample and a PCR reagent mixture onto the reaction spots; forming a sealed reaction chamber, having a volume of less than about 20 nanoliters, over each of the reaction spots; and amplifying the sample.

Abstract: A reclosable flexible package is suitable for retail sale of food products and is comprised of a film material. The reclosable flexible packages are suitable for mass production on high-speed form-fill-seal equipment. The flexible package includes a pair of semi-rigid strips attached to the walls of the flexible pouch and positioned parallel to the package opening. The semi-rigid strips are configured and arranged to nest together and bias toward one another. Upon opening of the package, the semi-rigid strips are moveable between a closed position and an open position. Further, the semi-rigid strips are configured such that they automatically reclose the package when the strips are released from the open position.

Abstract: A method for simultaneously determining a genetic expression profile for an individual member of a species relative to an entire standard genome for the species. The method can comprise distributing a liquid sample into an array of reaction chambers of a substrate. The array can comprise a primer set and a probe for each polynucleotide target along the entire standard genome. The liquid sample can comprise substantially all genetic material of the member. Each of the reaction chambers can comprise the primer set and the probe for at least one of the polynucleotide targets and a polymerase. The method can further comprise amplifying the liquid sample in the array, detecting a signal emitted by at least one of the probes, and identifying the genetic expression profile in response to the signal.

Abstract: New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available.

Abstract: A method of adjusting amplification curves in a PCR experiment includes receiving a plurality of amplification curves for a sample and computing a first parameter for each of the plurality of amplification curves. The method also includes computing a second parameter for each of the plurality of amplification curves and computing a third parameter using at least a portion of the first or second parameters. The method further includes computing an offset for each of the plurality of amplification curves. The offset is a function of the first parameter and the third parameter. Moreover, the method includes adjusting at least one of the plurality of amplification curves by subtracting the offset.

Abstract: A gas delivery system for a recreational vehicle comprising an inlet hose configured to be connected to a source of gas, a manifold connected to the inlet hose, a plurality of supply hoses connected to the manifold, and a plurality of appliances configured to be positioned within the recreational vehicle, with each one of the appliances being connected to one of the supply hoses. Gas can be supplied to each of the appliances via the inlet hose, the manifold and one of the supply hoses.