Abstract: Provided herein are methods of preparing a poloxamer for use in a cell culture medium. Also provided herein are cell culture media containing the poloxamer prepared by the methods herein, as well as methods of using the media for cell culturing and polypeptide production from cells.

Abstract: Games for teaching alphabet, numbers, colors, shapes and math along with coordination and motor skills comprising a planar member having a circle at its central interior and having curves therearound to define a plurality of zones around the center. The zones have radially extending lines therethrough, at least some of which are offset from each other to define segments with indicia in the form of an alphabet, number, color, and/or shape marked in each of the segments. A plurality of indicia is correlated to the indicia of the planar member, such plurality of indicia being randomly selectable by the players.