Patents by Inventor Robert J. Livingston
Robert J. Livingston has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11974546Abstract: Apparatuses, systems, and methods for the collection and sampling of agents (including organisms and pathogens) to determine the health of an animal colony. The apparatus includes a perforated housing that incorporates a collection media. The collection media is configured to collect a sample from a target animal, such as a mouse or rat, through the perforations of the housing when the target animal interacts with the perforated housing. The collected sample may include dust, feces, fur, urine, saliva, or other aspect of the target animal's environment.Type: GrantFiled: January 4, 2019Date of Patent: May 7, 2024Assignee: IDEXX Laboratories, Inc.Inventors: Matthew H. Myles, Marcus J. Crim, Robert S. Livingston, Cynthia L. Besch-Williford, Kristin S. Riley
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Patent number: 10894977Abstract: Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a template composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.Type: GrantFiled: January 8, 2019Date of Patent: January 19, 2021Assignee: Adaptive Biotechnologies corporationInventors: Harlan Saul Robins, Christopher Scott Carlson, Robert J. Livingston, Ryan O. Emerson, Anna M. Sherwood
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Publication number: 20190203281Abstract: Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a template composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.Type: ApplicationFiled: January 8, 2019Publication date: July 4, 2019Inventors: Harlan Saul ROBINS, Christopher Scott CARLSON, Robert J. LIVINGSTON, Ryan O. EMERSON, Anna M. SHERWOOD
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Patent number: 10214770Abstract: Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.Type: GrantFiled: May 10, 2016Date of Patent: February 26, 2019Assignee: ADAPTIVE BIOTECHNOLOGIES CORP.Inventors: Harlan Saul Robins, Christopher Scott Carlson, Robert J. Livingston, Ryan O. Emerson, Anna M. Sherwood
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Publication number: 20170335386Abstract: Compositions and methods for measuring adaptive immune receptor (T cell receptor and immunoglobulin) diversity are described, and find uses for assessing immunocompetence and other purposes. Means are provided for assessing the effects of diseases or conditions that compromise the immune system and of therapies aimed to reconstitute it. Lymphoid (B- and T-cell) adaptive immune receptor diversity is quantified by calculating the number of uniquely rearranged, CDR3-containing immunoglobulin (Ig) or T-cell receptor (TCR) variable region-encoding genes from sample cells such as blood cells.Type: ApplicationFiled: March 31, 2017Publication date: November 23, 2017Inventors: Robert J. Livingston, Christopher S. Carlson, Harlan S. Robins
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Publication number: 20170298100Abstract: Novel antiviral polypeptides are disclosed along with methods for their use to interfere with viral replication cycles by substantially impairing the binding of viruses to target cells, viral replication and assembly in infected cells, and viral egress from infected cells including viral lysis of host cells. The present antiviral peptides exhibit broad specificity across a range of human viral pathogens by virtue of their derivation from selected viral resistance genes and their ability to interfere with conserved mechanisms of host cell-virus interactions.Type: ApplicationFiled: September 30, 2015Publication date: October 19, 2017Inventor: Robert J. Livingston
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Publication number: 20160319340Abstract: Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.Type: ApplicationFiled: May 10, 2016Publication date: November 3, 2016Inventors: Harlan Saul Robins, Christopher Scott Carlson, Robert J. Livingston, Ryan O. Emerson, Anna M. Sherwood
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Patent number: 9371558Abstract: Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.Type: GrantFiled: January 9, 2015Date of Patent: June 21, 2016Assignee: Adaptive Biotechnologies Corp.Inventors: Harlan Saul Robins, Christopher Scott Carlson, Robert J. Livingston, Ryan O. Emerson, Anna M. Sherwood
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Patent number: 9279159Abstract: Compositions and methods are described for highly sensitive quantification of the relative representation of DNA from adaptive immune cells (e.g., T and/or B lymphocytes) in DNA extracted from complex mixtures of cells that include cells which are not adaptive immune cells. Included are methods for determining the relative presence in a tumor of tumor infiltrating lymphocytes (TIL), the relative presence of lymphocytes infiltrating a somatic tissue that is the target of an autoimmune disease, and the relative presence of lymphocytes infiltrating a transplanted organ.Type: GrantFiled: October 19, 2012Date of Patent: March 8, 2016Assignees: ADAPTIVE BIOTECHNOLOGIES CORPORATION, FRED HUTCHINSON CANCER RESEARCH CENTERInventors: Harlan S. Robins, Robert J. Livingston, Jason H. Bielas
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Patent number: 9181591Abstract: Compositions and methods are described for highly sensitive quantification of the relative representation of DNA from adaptive immune cells (e.g., T and/or B lymphocytes) in DNA extracted from complex mixtures of cells that include cells which are not adaptive immune cells. Included are methods for determining the relative presence in a tumor of tumor infiltrating lymphocytes (TIL), the relative presence of lymphocytes infiltrating a somatic tissue that is the target of an autoimmune disease, and the relative presence of lymphocytes infiltrating a transplanted organ.Type: GrantFiled: August 28, 2014Date of Patent: November 10, 2015Assignee: ADAPTIVE BIOTECHNOLOGIES CORPORATIONInventors: Harlan S. Robins, Robert J. Livingston
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Patent number: 9181590Abstract: Compositions and methods are described for highly sensitive quantification of the relative representation of DNA from adaptive immune cells (e.g., T and/or B lymphocytes) in DNA extracted from complex mixtures of cells that include cells which are not adaptive immune cells. Included are methods for determining the relative presence in a tumor of tumor infiltrating lymphocytes (TIL), the relative presence of lymphocytes infiltrating a somatic tissue that is the target of an autoimmune disease, and the relative presence of lymphocytes infiltrating a transplanted organ.Type: GrantFiled: March 6, 2014Date of Patent: November 10, 2015Assignee: ADAPTIVE BIOTECHNOLOGIES CORPORATIONInventors: Harlan S. Robins, Robert J. Livingston
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Publication number: 20150299785Abstract: Compositions and methods for measuring adaptive immune receptor (T cell receptor and immunoglobulin) diversity are described, and find uses for assessing immunocompetence and other purposes. Means are provided for assessing the effects of diseases or conditions that compromise the immune system and of therapies aimed to reconstitute it. Lymphoid (B- and T-cell) adaptive immune receptor diversity is quantified by calculating the number of uniquely rearranged. CDR3-containing immunoglobulin (Ig) or T-cell receptor (TCR) variable region-encoding genes from sample cells such as blood cells.Type: ApplicationFiled: March 6, 2015Publication date: October 22, 2015Inventors: Robert J. Livingston, Christopher S. Carlson, Harlan S. Robins
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Patent number: 9150905Abstract: Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.Type: GrantFiled: May 8, 2013Date of Patent: October 6, 2015Assignee: Adaptive Biotechnologies CorporationInventors: Harlan Saul Robins, Christopher Scott Carlson, Robert J. Livingston, Ryan O. Emerson, Anna M. Sherwood
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Publication number: 20150203897Abstract: Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed N high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.Type: ApplicationFiled: January 9, 2015Publication date: July 23, 2015Inventors: HARLAN SAUL ROBINS, CHRISTOPHER SCOTT CARLSON, ROBERT J. LIVINGSTON, RYAN O. EMERSON, ANNA M. SHERWOOD
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Publication number: 20150051089Abstract: Compositions and methods are described for highly sensitive quantification of the relative representation of DNA from adaptive immune cells (e.g., T and/or B lymphocytes) in DNA extracted from complex mixtures of cells that include cells which are not adaptive immune cells. Included are methods for determining the relative presence in a tumor of tumor infiltrating lymphocytes (TIL), the relative presence of lymphocytes infiltrating a somatic tissue that is the target of an autoimmune disease, and the relative presence of lymphocytes infiltrating a transplanted organ.Type: ApplicationFiled: August 28, 2014Publication date: February 19, 2015Inventors: Harlan S. Robins, Robert J. Livingston
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Publication number: 20150017652Abstract: Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.Type: ApplicationFiled: May 8, 2013Publication date: January 15, 2015Inventors: Harlan Saul Robins, Christopher Scott Carlson, Robert J. Livingston
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Publication number: 20140186848Abstract: Compositions and methods are described for highly sensitive quantification of the relative representation of DNA from adaptive immune cells (e.g., T and/or B lymphocytes) in DNA extracted from complex mixtures of cells that include cells which are not adaptive immune cells. Included are methods for determining the relative presence in a tumor of tumor infiltrating lymphocytes (TIL), the relative presence of lymphocytes infiltrating a somatic tissue that is the target of an autoimmune disease, and the relative presence of lymphocytes infiltrating a transplanted organ.Type: ApplicationFiled: March 6, 2014Publication date: July 3, 2014Applicants: Fred Hutchinson Cancer Research Center, Adaptive Biotechnologies CorporationInventors: Harlan S. Robins, Robert J. Livingston, Jason H. Bielas
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Publication number: 20120058902Abstract: Compositions and methods for measuring adaptive immune receptor (T cell receptor and immunoglobulin) diversity are described, and find uses for assessing immunocompetence and other purposes. Means are provided for assessing the effects of diseases or conditions that compromise the immune system and of therapies aimed to reconstitute it. Lymphoid (B- and T-cell) adaptive immune receptor diversity is quantified by calculating the number of uniquely rearranged, CDR3-containing immunoglobulin (Ig) or T-cell receptor (TCR) variable region-encoding genes from sample cells such as blood cells.Type: ApplicationFiled: August 24, 2011Publication date: March 8, 2012Inventors: Robert J. Livingston, Christopher S. Carlson, Harlan S. Robins
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Patent number: 6643938Abstract: A replacement string arrangement for a string trimmer employs a brace in which the string is affixed. The brace has an arcuate member with an annular guide tunnel or guide sleeve at one end. The cutting string has an end affixed at a second end of the arcuate member. The string extends out that one end, then forms a loop or bight, and then passes slidably through the guide tunnel and continues radially outward. A free end of the string projects from the cutting head when the replacement string arrangement is installed in the cutting head. The brace and string can be unitarily formed, or the string can be fused into the one end of the arcuate member. The brace may have a wishbone shape. The string profile or cross section may be shaped for improved aerodynamics or improved cutting.Type: GrantFiled: April 23, 2002Date of Patent: November 11, 2003Inventor: Robert J. Livingston
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Publication number: 20030033721Abstract: A replacement string arrangement for a string trimmer employs a brace in which the string is affixed. The brace has an arcuate member with an annular guide tunnel or guide sleeve at one end. The cutting string has an end affixed at a second end of the arcuate member. The string extends out that one end, then forms a loop or bight, and then passes slidably through the guide tunnel and continues radially outward. A free end of the string projects from the cutting head when the replacement string arrangement is installed in the cutting head. The brace and string can be unitarily formed, or the string can be fused into the one end of the arcuate member. The brace may have a wishbone shape. The string profile or cross section may be shaped for improved aerodynamics or improved cutting.Type: ApplicationFiled: April 23, 2002Publication date: February 20, 2003Inventor: Robert J. Livingston