Patents by Inventor Robert M. Horton

Robert M. Horton has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for gene splicing by overlap extension using the polymerase chain reaction
    Patent number: 5023171
    Abstract: A method is provided for joining two DNA molecules by first amplifying them by means of polymerase chain reactions (PCR) carried out on each molecule using oligonucleotide primers designed so that the ends of the resultant PCR products contain complementary sequences. When the two PCR products are mixed, denatured and reannealed, the single-stranded DNA strands having the complementary sequences at their 3' ends anneal and then act as primers for each other. Extension of the annealed area by DNA polymerase produces a double-stranded DNA molecule in which the original molecules are spliced together.
    Type: Grant
    Filed: August 10, 1989
    Date of Patent: June 11, 1991
    Assignee: Mayo Foundation for Medical Education and Research
    Inventors: Steffan N. Ho, Robert M. Horton