Patents by Inventor Robert Marcil
Robert Marcil has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20120322109Abstract: The present invention provides a method of covalently joining a DNA strand to an RNA strand, a method of tagging a 5? end of an RNA molecule, a DNA-RNA molecule which has been joined in vitro by the use of a topoisomerase, a method of tagging a 5? end of an mRNA, and a method of isolating and cloning full-length gene sequences using capped mRNA after subtraction of non-capped RNA.Type: ApplicationFiled: April 30, 2012Publication date: December 20, 2012Inventors: STEWART SHUMAN, JOANN SEKIGUCHI, JOHN COMISKEY, JOSEPH FERNANDEZ, JAMES HOEFFLER, ROBERT MARCIL
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Patent number: 8192932Abstract: The present invention provides a method of covalently joining a DNA strand to an RNA strand, a method of tagging a 5? end of an RNA molecule, a DNA-RNA molecule which has been joined in vitro by the use of a topoisomerase, a method of tagging a 5? end of an mRNA, and a method of isolating and cloning full-length gene sequences using capped mRNA after subtraction of non-capped RNA.Type: GrantFiled: February 12, 2010Date of Patent: June 5, 2012Assignees: Sloan-Kettering Institute For Cancer Research, Invitrogen CorporationInventors: Stewart Shuman, JoAnn Sekiguchi, John Comiskey, Joseph Fernandez, James Hoeffler, Robert Marcil
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Publication number: 20100317064Abstract: The present invention provides a method of covalently joining a DNA strand to an RNA strand comprising (a) forming a topoisomerase-DNA intermediate by incubating a DNA cleavage substrate comprising a topoisomerase cleavage site with a topoisomerase specific for that site, wherein the topoisomerase-DNA intermediate has one or more 5? single-strand tails; and (b) adding to the topoisomerase-DNA intermediate an acceptor RNA strand complementary to the 5? single-strand tail under conditions permitting a ligation of the covalently bound DNA strand of the topoisomerase-DNA intermediate to the RNA acceptor strand and dissociation of the topoisomerase, thereby covalently joining the DNA strand to the RNA strand. The present invention also provides a method of tagging a 5? end of an RNA molecule. The present invention further provides a DNA-RNA molecule which has been joined in vitro by the use of a topoisomerase. The present invention also provides a method of tagging a 5? end of an mRNA.Type: ApplicationFiled: February 12, 2010Publication date: December 16, 2010Inventors: Stewart Shuman, JoAnn Sekiguchi, John Comiskey, Joseph Fernandez, James Hoeffler, Robert Marcil
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Patent number: 7662556Abstract: The present invention provides a method of covalently joining a DNA strand to an RNA strand comprising (a) forming a topoisomerase-DNA intermediate by incubating a DNA cleavage substrate comprising a topoisomerase cleavage site with a topoisomerase specific for that site, wherein the topoisomerase-DNA intermediate has one or more 5? single-strand tails; and (b) adding to the topoisomerase-DNA intermediate an acceptor RNA strand complementary to the 5? single-strand tail under conditions permitting a ligation of the covalently bound DNA strand of the topoisomerase-DNA intermediate to the RNA acceptor strand and dissociation of the topoisomerase, thereby covalently joining the DNA strand to the RNA strand. The present invention also provides a method of tagging a 5? end of an RNA molecule. The present invention further provides a DNA-RNA molecule which has been joined in vitro by the use of a topoisomerase. The present invention also provides a method of tagging a 5? end of an mRNA.Type: GrantFiled: September 19, 2003Date of Patent: February 16, 2010Assignee: Sloan Kettering Institute for Cancer ResearchInventors: Stewart Shuman, JoAnn Sekiguchi, Joseph Fernandez, Robert Marcil, James Hoeffler, John Comiskey
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Publication number: 20070128724Abstract: The present invention is a cell-free subcloning system utilizing three elements: (1) a donor vector that contains a nucleic acid sequence to be transferred to another vector flanked by a site-specific recombination sequence and one or more optional additional nucleic acid sequences, (2) an acceptor vector that contains a site-specific recombination sequence and one or more optional additional nucleic acid sequences, and (3) a site-specific recombinase that recognizes the site-specific recombination sequences in the donor and acceptor vectors so as to transfer the transfer sequence from the donor to the acceptor vector upon contact of the three elements of the system. Also disclosed are rapid subcloning methods employing the vectors and enzymes disclosed herein and kits for use in such methods.Type: ApplicationFiled: January 27, 2006Publication date: June 7, 2007Applicant: Invitrogen CorporationInventors: David Miles, Lyle Turner, Robert Marcil, Gina McConnell
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Publication number: 20050181417Abstract: The present invention is a cell-free subcloning system utilizing three elements: (1) a donor vector that contains a nucleic acid sequence to be transferred to another vector flanked by a site-specific recombination sequence and one or more optional additional nucleic acid sequences, (2) an acceptor vector that contains a site-specific recombination sequence and one or more optional additional nucleic acid sequences, and (3) a site-specific recombinase that recognizes the site-specific recombination sequences in the donor and acceptor vectors so as to transfer the transfer sequence from the donor to the acceptor vector upon contact of the three elements of the system. Also disclosed are rapid subcloning methods employing the vectors and enzymes disclosed herein and kits for use in such methods.Type: ApplicationFiled: March 16, 2005Publication date: August 18, 2005Applicant: Invitrogen CorporationInventors: David Miles, Lyle Turner, Robert Marcil, Gina McConnell
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Publication number: 20040058417Abstract: The present invention provides a method of covalently joining a DNA strand to an RNA strand comprising (a) forming a topoisomerase-DNA intermediate by incubating a DNA cleavage substrate comprising a topoisomerase cleavage site with a topoisomerase specific for that site, wherein the topoisomerase-DNA intermediate has one or more 5′ single-strand tails; and (b) adding to the topoisomerase-DNA intermediate an acceptor RNA strand complementary to the 5′ single-strand tail under conditions permitting a ligation of the covalently bound DNA strand of the topoisomerase-DNA intermediate to the RNA acceptor strand and dissociation of the topoisomerase, thereby covalently joining the DNA strand to the RNA strand. The present invention also provides a method of tagging a 5′ end of an RNA molecule. The present invention further provides a DNA-RNA molecule which has been joined in vitro by the use of a topoisomerase. The present invention also provides a method of tagging a 5′ end of an mRNA.Type: ApplicationFiled: September 19, 2003Publication date: March 25, 2004Applicant: Sloan Kettering Institute for Cancer ResearchInventors: Stewart Shuman, JoAnn Sekiguchi, John Comiskey, Joseph Fernandez, James Hoeffler, Robert Marcil
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Publication number: 20030153055Abstract: The present invention is a cell-free subcloning system utilizing three elements: (1) a donor vector that contains a nucleic acid sequence to be transferred to another vector flanked by a site-specific recombination sequence and one or more optional additional nucleic acid sequences, (2) an acceptor vector that contains a site-specific recombination sequence and one or more optional additional nucleic acid sequences, and (3) a site-specific recombinase that recognizes the site-specific recombination sequences in the donor and acceptor vectors so as to transfer the transfer sequence from the donor to the acceptor vector upon contact of the three elements of the system. Also disclosed are rapid subcloning methods employing the vectors and enzymes disclosed herein and kits for use in such methods.Type: ApplicationFiled: February 20, 2003Publication date: August 14, 2003Applicant: Invitrogen CorporationInventors: David J. Miles, Lyle C. Turner, Robert Marcil, Gina C. McConnell
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Publication number: 20020106797Abstract: The present invention is a cell-free subcloning system utilizing three elements: (1) a donor vector that contains a nucleic acid sequence to be transferred to another vector flanked by a site-specific recombination sequence and one or more optional additional nucleic acid sequences, (2) an acceptor vector that contains a site-specific recombination sequence and one or more optional additional nucleic acid sequences, and (3) a site-specific recombinase that recognizes the site-specific recombination sequences in the donor and acceptor vectors so as to transfer the transfer sequence from the donor to the acceptor vector upon contact of the three elements of the system. Also disclosed are rapid subcloning methods employing the vectors and enzymes disclosed herein and kits for use in such methods.Type: ApplicationFiled: March 12, 2001Publication date: August 8, 2002Applicant: INVITROGEN CORPORATIONInventors: David J. Miles, Lyle C. Turner, Robert Marcil, Gina C. McConnell