Abstract: A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.

Abstract: A radio controlled timepiece movement includes a carrier structure and a wheel train mounted thereon. The wheel train includes a plurality of rotatable wheels each having a wheel aperture formed therein. The apertures are alignable with one another. A printed circuit board is mounted on the carrier structure and has a board aperture formed therethrough. An end of the board aperture disposed farthest from the wheels is covered by a portion of a conductor of the circuit board. A light beam transmitter is mounted on that conductor and disposed in the board aperture. A wire extends from the element to the second conductor. The transmitter and wire are embedded within a radiation-permeable material which fills the board aperture.

Abstract: An autonomous radio timepiece (11) with a magnetic antenna (14) located in the vicinity of a electromechanical transducer (25) for driving a time display (19), in order to obtain a radio timepiece with a small volume and operable in a compact manner. To avoid electromagnetic interference with the antenna inlet of a tuned radio-frequency receiver (12), the transducer (25) is deactivated while the receiver (12) is actuated for the reception and coding of an absolute time information (15) transmitted by radio. A subsequent recovery of the transducer drive pulses (42) for autonomous time keeping corrects the display (19). However, a transducer (25.

Abstract: A radio-controlled timepiece contains a mechanism for the detection and correction of a hands setting. The mechanism includes a sender and a receiver for sending and receiving, respectively, a radiation beam. A hands setting mechanism includes an hour wheel, a minute wheel, and a seconds wheel, each possessing an aperture therethrough. The hour wheel has a front mirror located proximate the beam sender for reflecting a beam to the receiver. The front mirror has an interruption defined by the aperture of the hour disk in order to pass the beam through that aperture to a rear mirror disposed remote from the sender, such that a beam reflects from the rear mirror only after passing through aligned apertures in the disks. The hour wheel is movable independently of the minute wheel and seconds wheel, and the minute wheel and seconds wheel are rotatably interconnected.

Abstract: A radio-controlled timepiece includes a clock face, a hands assembly mounted for rotation about an axis in front of the clock face, and an alarm setting mechanism. The alarm setting mechanism comprises a minute setting disk which is driven by an hour setting disk. A manually rotatable transparent support disk is mounted in front of the clock face for rotation about the same axis as the hands assembly. The support disk carries a marker which, when the support member is rotated, is moved to indicate a particular hour and minute indicator on the clock face. The support disk is connected to the hour setting disk to rotate the latter which, in turn, rotates the minute setting disk. The support disk is accessible to rotation at the front of the clock. The hour and minute setting disks are preferably connected to an alarm time storage device by means of conductive fingers which are mounted on the disks and which are engageable with concentric conductive strips mounted on a stationary part of the clock.

Abstract: An autonomous radio timepiece comprises a casing and a bracelet attached thereto. The casing includes an operating circuit, while the bracelet carries a flexible antenna. The antenna can be removed from the bracelet in the event that the bracelet is worn and must be discarded. The antenna carries a plug-in type connector at one end which is adapted to make a tight sealing connection with the casing, and is also adapted to electrically connect the antenna with the circuit of the casing. The circuit within the casing includes a receiver which is fixedly tuned as a function of the antenna inductivity of a foil core to the time message transmitter. Also provided is a variable automatic supplemental tuner to compensate for bending of the bracelet while the receiver is actuated. The antenna comprises a foil of an insulting material on which an antenna conductor is printed, along with two shielding conductors extending parallel to the antenna conductor.

Abstract: An autonomous radio timepiece having a time equalizing processor to control and potentially correct the instantaneous time indication on the basis of the prevailing time information received by radio transmission, is equipped with an improved device for temporarily interrupting operation of the time indication. Such a device is provided for the purpose of interrupting the display function of the radio timepiece without deactivating the other auxiliary and operating functions of the radio timepiece, so that following termination of the interruption, normal operation continues without any interference. The display may be interrupted indirectly or directly. In the first case the reset inlet of the time equalizing processor is actuated to trigger the synchronizing process, which otherwise takes place upon the actuation of the radio timepiece.

Abstract: A clock movement comprises front and rear housing shells. A support plate is mounted within and between the shells. A gear movement is mounted on a rear side of the support plate and faces the rear shell. A hands movement is arranged on a front side of the support plate and is drivingly connected to the gear movement. A circuit board is connected to the support plate. A motor for the gear movement comprises a stator sheet and a coil bobbin. The stator sheet is fastened in engaging relationship to a rear side of the support plate adjacent a rotor opening in the stator sheet. The coil bobbin is mounted on the stator sheet and includes a plurality of pins which define coil terminals. The pins are soldered to a circuit of the circuit board. The circuit board is disposed in front of the support plate in spaced relationship therewith and is of arc-like configuration such that legs of the arc straddle the minute and hour wheels of the hands movement.

Abstract: A timepiece drive assembly is adapted to be mounted in a timepiece housing, e.g., a small designer desk clock. The assembly includes a movement casing equipped with a removable battery-receiving member in which battery contact terminals are releasably arranged. The contact terminals are connected by means of flexible connecting lines with the circuit board of the movement. The movement casing may be arranged in a cylindrical part of the clock housing, while the battery-receiving member is located in a pedestal provided underneath the cylindrical part. It is only necessary to equip the pedestal with fastening means for the battery-receiving member and not with the more expensive individual fastening means for the holding and contacting of the battery. Thus, substantially flexibility is obtained in the design of the timepiece housing because the battery-receiving member can be connected to the movement casing or spaced therefrom.

Abstract: A contact arrangement for a battery-operated instrument, especially a battery-operated clock, having a housing made of synthetic material. Two contact elements are provided for making connection between the two terminals of a battery and two respective paths of a circuit board. One of the contact elements includes a bus portion fastened to the housing, and a pair of legs. A first leg makes contact with one terminal of the battery and the second leg makes contact with a path of the circuit board. The second leg is designed in the form of a knife-edge contact, one portion thereof bearing against a wall of the housing, and another portion thereof bearing against the circuit board. The second leg thus holds the circuit board in its inserted position by mechanical force. The contact element further includes a third leg which electrically contacts as well as mechanically clamps a small bulb when the latter is inserted into the housing.

Abstract: An arrangement is disclosed for the disassembly from a clock housing of a circuit board which carries various electromechanical and electronic components. The novel disassembly arrangement comprises an ejection device which carries several ejecting posts which can be pushed through corresponding openings in a wall of the housing to press against the circuit board and/or at least a portion of the components fastened to the board, thereby detaching this circuit board from its mounts. The circuit board is held in its position by the frictional force exerted by a contact spring which is designed in the shape of a knife-edge contact so that it will only be necessary to overcome the force of the spring during the ejection operation. The novel disassembly device, made available by the clock manufacturer for use by craftsmen, will make it possible for such person to remove the circuit board and its components rapidly and with very great ease in the case of making necessary repairs.

Abstract: A clock is disclosed comprising a housing which includes mounts for supporting clockhands, and a clockwork for driving the hands. The clock also includes a motor, an electronic circuitry for conducting electrical power to the motor, and a circuit board. The motor includes a coil, a stator, and a rotor. The circuit board includes means for carrying the electronic circuitry, coil and stator externally of the housing to form a pre-assembled module which can be pushed into the housing into contact with respective retaining parts on the housing.

Abstract: An electrical contact arrangement is disclosed for a battery-operated instrument, especially a battery-operated clock having a housing made of synthetic material such as plastic. Two contact elements are provided for connecting two terminals of the battery with two respective paths of a circuit board. One of the contact elements comprises a bus fastened to the plastic housing, and several legs. A first leg makes contact with one terminal of the battery and the second leg makes contact with a path of the circuit board. The second leg is designed in the form of a knife-edge contact with a first portion bearing against a wall of the housing and a second portion bearing against the circuit board, the latter having been inserted into the housing parallel with such wall. The second portion of the contact engages an appropriate path of the board.

Abstract: A drive arrangement within a small electric instrument, especially a battery-operated electronic clock, includes a circuit board. Mounted on the circuit board, in addition to the electronic circuitry, are at least the static components of a motor which can be mounted on the circuit board prior to its installation. The circuit board and such static components can thus be inserted as a unit into the clock housing and into suitably designed receiving elements. The receiving elements position the circuit board, as well as the components arranged thereon, into the proper locational relation to the other parts of the motor or clock. The circuit board is held in place after its insertion into the housing by a battery contact spring which is designed in the form of a knife-edged contact and which presses laterally against the circuit board, thus holding the board in its inserted position without the need for additional fastening means.