Patents by Inventor Ron Milo
Ron Milo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240117386Abstract: A recombinant bacteria which is genetically modified to express formate dehydrogenase (FDH), phosphoribulokinase (prk) and Ribulose-Bisphosphate Carboxylase/oxygenase (RuBisCo) is disclosed. The bacteria may be modified to be autotrophic.Type: ApplicationFiled: October 22, 2020Publication date: April 11, 2024Applicant: Yeda Research and Development Co. Ltd.Inventors: Ron MILO, Shmuel GLEIZER, Niv ANTONOVSKY, Elad NOOR, Arren BAR-EVEN, Yehudit ZOHAR, Roee BEN NISSAN, Elad HERZ, Yinon Moise BAR-ON
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Patent number: 10377994Abstract: An isolated microorganism that expresses enzymes of the reductive glycine pathway is disclosed. The microorganism is capable of converting formate to pyruvate or glycerate via the formation of glycine and serine. Methods of generating same are further described.Type: GrantFiled: October 17, 2018Date of Patent: August 13, 2019Assignee: Yeda Research and Developmetn Co. Ltd.Inventors: Arren Bar-Even, Ron Milo, Elad Noor, Oren Yishai
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Publication number: 20190211342Abstract: A microorganism which is genetically modified so that it produces a first essential biomass precursor by metabolizing CO2 using a recombinant carbon fixation enzyme is disclosed. The microorganism produces a second biomass precursor by metabolizing an organic carbon source and not by metabolizing CO2. The microorganism does not use the organic carbon source for producing the first essential biomass precursor.Type: ApplicationFiled: March 25, 2019Publication date: July 11, 2019Applicant: Yeda Research and Development Co. Ltd.Inventors: Ron MILO, Niv ANTONOVSKY, Elad NOOR, Arren BAR-EVEN, Yehudit ZOHAR, Lior ZELCBUCH, Shmuel GLEIZER, Shira AMRAM
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Patent number: 10294482Abstract: A microorganism which is genetically modified so that it produces a first essential biomass precursor by metabolizing CO2 using a recombinant carbon fixation enzyme is disclosed. The microorganism produces a second biomass precursor by metabolizing an organic carbon source and not by metabolizing CO2. The microorganism does not use the organic carbon source for producing the first essential biomass precursor.Type: GrantFiled: May 21, 2015Date of Patent: May 21, 2019Assignee: Yeda Research and Development Co. Ltd.Inventors: Ron Milo, Niv Antonovsky, Elad Noor, Arren Bar-Even, Yehudit Zohar, Lior Zelcbuch, Shmuel Gleizer, Shira Amram
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Publication number: 20190032027Abstract: An isolated microorganism that expresses enzymes of the reductive glycine pathway is disclosed. The microorganism is capable of converting formate to pyruvate or glycerate via the formation of glycine and serine. Methods of generating same are further described.Type: ApplicationFiled: October 17, 2018Publication date: January 31, 2019Applicant: Yeda Research and Development Co. Ltd.Inventors: Arren Bar-Even, Ron Milo, Elad Noor, Oren Yishai
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Patent number: 10155933Abstract: An isolated microorganism that expresses enzymes of the reductive glycine pathway is disclosed. The microorganism is capable of converting formate to pyruvate or glycerate via the formation of glycine and serine. Methods of generating same are further described.Type: GrantFiled: July 29, 2013Date of Patent: December 18, 2018Assignee: Yeda Research and Development Co., Ltd.Inventors: Arren Bar-Even, Ron Milo, Elad Noor, Oren Yishai
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Patent number: 10077437Abstract: A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.Type: GrantFiled: June 22, 2016Date of Patent: September 18, 2018Assignee: Yeda Research and Development Co. Ltd.Inventors: Ron Milo, Arren Bar-Even, Elad Noor
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Patent number: 10053712Abstract: An isolated microorganism is disclosed being genetically modified to express pyruvate formate lyase (PFL) or 2-ketobutyrate formate lyase, wherein acetyl-CoA of the microorganism is converted to pyruvate in the presence of formate in a single step reaction, wherein the net flux of the reaction is in the direction of pyruvate synthesis. Uses of the microorganism and products comprising same are also disclosed.Type: GrantFiled: December 10, 2014Date of Patent: August 21, 2018Assignee: Yeda Research and Development Co. Ltd.Inventors: Arren Bar-Even, Ron Milo, Elad Noor, Lior Zelcbuch
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Publication number: 20170268030Abstract: A plurality of isolated polynucleotide sequences encoding enzymes of the astaxanthin pathway is disclosed. The polynucleotides include: (i) a polynucleotide which encodes Phytoene dehydrogenase (crtI) and a first transcriptional regulatory sequence; (ii) a polynucleotide which encodes Beta-lycopene cyclase (lcy-B) and a second transcriptional regulatory sequence; (iii) a polynucleotide which encodes Beta-carotene ketolase (crtW) and a third transcriptional regulatory sequence; and wherein the first, second and third regulatory sequence are selected such that the expression of the Icy-B and the crtW is greater than a level of expression of the crtI. Methods of generating astaxanthin using the plurality of polynucleotide are also disclosed as well as bacterial cells comprising high levels of astaxanthin.Type: ApplicationFiled: June 2, 2017Publication date: September 21, 2017Inventors: Ron MILO, Lior ZELCBUCH, Niv ANTONOVSKY
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Patent number: 9719122Abstract: A plurality of isolated polynucleotide sequences encoding enzymes of the astaxanthin pathway is disclosed. The polynucleotides include: (i) a polynucleotide which encodes Phytoene dehydrogenase (crtI) and a first transcriptional regulatory sequence; (ii) a polynucleotide which encodes Beta-lycopene cyclase (lcy-B) and a second transcriptional regulatory sequence; (iii) a polynucleotide which encodes Beta-carotene ketolase (crtW) and a third transcriptional regulatory sequence; and wherein the first, second and third regulatory sequence are selected such that the expression of the Icy-B and the crtW is greater than a level of expression of the crtI. Methods of generating astaxanthin using the plurality of polynucleotide are also disclosed as well as bacterial cells comprising high levels of astaxanthin.Type: GrantFiled: July 17, 2013Date of Patent: August 1, 2017Assignee: Yeda Research and Development Co. Ltd.Inventors: Ron Milo, Lior Zelcbuch, Niv Antonovsky
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Publication number: 20170183665Abstract: A microorganism which is genetically modified so that it produces a first essential biomass precursor by metabolizing CO2 using a recombinant carbon fixation enzyme is disclosed. The microorganism produces a second biomass precursor by metabolizing an organic carbon source and not by metabolizing CO2. The microorganism does not use the organic carbon source for producing the first essential biomass precursor.Type: ApplicationFiled: May 21, 2015Publication date: June 29, 2017Inventors: Ron MILO, Niv ANTONOVSKY, Elad NOOR, Arren BAR-EVEN, Yehudit ZOHAR, Lior ZELCBUCH, Shmuel GLEIZER, Shira AMRAM
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Publication number: 20170081682Abstract: An isolated microorganism is disclosed being genetically modified to express pyruvate formate lyase (PFL) or 2-ketobutyrate formate lyase, wherein acetyl-CoA of the microorganism is converted to pyruvate in the presence of formate in a single step reaction, wherein the net flux of the reaction is in the direction of pyruvate synthesis. Uses of the microorganism and products comprising same are also disclosed.Type: ApplicationFiled: December 10, 2014Publication date: March 23, 2017Applicant: Yeda Research and Development Co. Ltd.Inventors: Arren BAR-EVEN, Ron MILO, Elad NOOR, Lior ZELCBUCH
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Publication number: 20160298103Abstract: A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.Type: ApplicationFiled: June 22, 2016Publication date: October 13, 2016Applicant: Yeda Research and Development Co. Ltd.Inventors: Ron MILO, Arren BAR-EVEN, Elad NOOR
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Patent number: 9410131Abstract: A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.Type: GrantFiled: February 10, 2011Date of Patent: August 9, 2016Assignee: Yeda Research and Development Co. Ltd.Inventors: Ron Milo, Arren Bar-Even, Elad Noor
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Publication number: 20150218528Abstract: An isolated microorganism that expresses enzymes of the reductive glycine pathway is disclosed. The microorganism is capable of converting formate to pyruvate or glycerate via the formation of glycine and serine. Methods of generating same are further described.Type: ApplicationFiled: July 29, 2013Publication date: August 6, 2015Inventors: Arren Bar-Even, Ron Milo, Elad Noor, Oren Yishai
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Publication number: 20150211040Abstract: A plurality of isolated polynucleotide sequences encoding enzymes of the astaxanthin pathway is disclosed. The polynucleotides include: (i) a polynucleotide which encodes Phytoene dehydrogenase (crtI) and a first transcriptional regulatory sequence; (ii) a polynucleotide which encodes Beta-lycopene cyclase (lcy-B) and a second transcriptional regulatory sequence; (iii) a polynucleotide which encodes Beta-carotene ketolase (crtW) and a third transcriptional regulatory sequence; and wherein the first, second and third regulatory sequence are selected such that the expression of the Icy-B and the crtW is greater than a level of expression of the crtI. Methods of generating astaxanthin using the plurality of polynucleotide are also disclosed as well as bacterial cells comprising high levels of astaxanthin.Type: ApplicationFiled: July 17, 2013Publication date: July 30, 2015Inventors: Ron Milo, Lior Zelcbuch, Niv Antonovsky
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Publication number: 20120301947Abstract: A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.Type: ApplicationFiled: February 10, 2011Publication date: November 29, 2012Applicant: Yeda Research and Development Co. Ltd.Inventors: Ron Milo, Arren Bar-Even, Elad Noor
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Publication number: 20100298166Abstract: Nucleic acid construct systems are disclosed. The constructs comprise: (i) a first nucleic acid construct comprising a first nucleic acid sequence encoding a first reporter polypeptide linked to an additional nucleic acid sequence capable of inserting the first nucleic acid construct into a genome of a host cell such that an endogenous polypeptide covalently attached to the first reporter polypeptide is expressed in the cell; and (ii) a second nucleic acid construct comprising a second nucleic acid sequence encoding a second reporter polypeptide, linked to an additional nucleic acid sequence capable of inserting in a non-directed manner the second nucleic acid construct into a genome of a host cell such that an endogenous polypeptide covalently attached to the second reporter polypeptide is expressed in the cell, wherein the first reporter polypeptide and the second reporter polypeptide are distinguishable.Type: ApplicationFiled: January 22, 2009Publication date: November 25, 2010Inventors: Uri Alon, Alex Sigal, Ron Milo, Tamar Danon, Ariel Cohen, Naama Geva-Zatorsky, Milana Frenkel-Morgenstern, Lydia Cohen, Natalie Perzov, Eran Eden
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Publication number: 20040204925Abstract: A method for analyzing data, such as biological data for example, for identifying one or more network motifs, or recurring patterns of relationships and/or behavioral connections between the components of a complex system. The method of the present invention is optionally and preferably applied to biological systems, such as gene regulatory systems for example.Type: ApplicationFiled: December 29, 2003Publication date: October 14, 2004Inventors: Uri Alon, Shalev Itzkovitz, Reuven Levitt, Nadav Kashtan, Ron Milo