Abstract: A fiber optic coupler for use with a diode array spectrophotometer system that optimizes the optical interface between a first fiber optic waveguide employed to couple light from a sample under analysis and a diode array spectrograph. The coupler comprises a fiber optic waveguide connector that secures the first optical fiber waveguide that couples light from the sample under investigation. A slit block is provided that has a relatively thin, rectangular exit aperture having dimensions compatible with the spectrograph. A single waveguide (or plurality of fiber optic waveguides) is coupled between the connector and the exit aperture and is arranged to have a generally round cross section adjacent the connector and a linear cross section adjacent the spectrograph. Improved light throughput is achieved at the fiber optic waveguide-spectrophotometer interface, when compared with conventional fiber optic spectrophotometer designs.