Abstract: Systems and methods for analyzing multiple samples in parallel using mass spectrometry preferably coupled with fluid phase separation techniques are provided. A modular mass spectrometer includes a vacuum enclosure, multiple sample inlets, multiple common vacuum pumping elements, and multiple mass analysis modules disposed substantially within the enclosure, with each module preferably including a mass analyzer and a transducer. In one embodiment, the modules mate with the vacuum enclosure to define multiple sequential vacuum regions, with each vacuum region having an associated common vacuum pumping element. At least one multi-pole ion transfer optic element is preferably associated with each module. Fluid phase separation devices may include microfluidic devices utilizing chromatographic, electrophoretic, or other separation methods.

Abstract: A pressure-driven microfluidic device for separating chemical or biological species from a sample provides an on-board stationary phase packing manifold or distribution network for simultaneously packing multiple separation channels. The packing manifold or distribution may include both a stationary phase inlet port and a vent port, and the vent port may include an associated porous material or frit. Methods for operating pressure-driven microfluidic separation devices include the steps of venting the packing manifold to an environment having a lower pressure than that present in the separation columns to allow any retained sample portions to migrate away from the separation channels, thereby minimizing or eliminating cross-talk and sample contamination.