Patents by Inventor Ronald M. Cook
Ronald M. Cook has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8440399Abstract: The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.Type: GrantFiled: August 25, 2009Date of Patent: May 14, 2013Assignee: Biosearch Technologies, Inc.Inventors: Ronald M. Cook, Matt Lyttle, Daren Dick
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Patent number: 8410255Abstract: The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.Type: GrantFiled: August 6, 2010Date of Patent: April 2, 2013Assignee: Biosearch Technologies, Inc.Inventors: Ronald M. Cook, Matt Lyttle, Daren Dick
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Publication number: 20120208190Abstract: The present invention provides luminescent metal ion complexes for use in a wide range of biological and chemical studies. The luminescent metal ion complexes of the invention comprise a metal ion chelating component covalently bound to a carrier molecule. Also provided are methods of making and using the luminescent metal ion complexes.Type: ApplicationFiled: January 23, 2012Publication date: August 16, 2012Applicant: Biosearch Technologies, Inc.Inventors: Ronald M. Cook, Matt Lyttle, Mary Katherine Johannson
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Patent number: 8119781Abstract: The present invention provides luminescent metal ion complexes for use in a wide range of biological and chemical studies. The luminescent metal ion complexes of the invention comprise a metal ion chelating component covalently bound to a carrier molecule. Also provided are methods of making and using the luminescent metal ion complexes.Type: GrantFiled: December 21, 2009Date of Patent: February 21, 2012Assignee: Biosearch Technologies, Inc.Inventors: Ronald M. Cook, Matt Lyttle, Mary Katherine Johannson
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Publication number: 20110282041Abstract: The present invention provides a new class of solids supports for synthesis of modified oligomers of nucleic acids, and nucleic acid probes that have a format expediently synthesized on the new supports. Exemplary solid supports include at least one quencher bound through a linker to the solid support. Various exemplary embodiments include a moiety that stabilizes a duplex, triplex or higher order aggregation (e.g., hybridization) of nucleic acids of which the oligomer of the invention is a component. Other components of the solid support include moieties that stabilize aggregations of nucleic acids, e.g., intercalators, minor groove binding moieties, bases modified with a stabilizing moiety (e.g., alkynyl moieties, and fluoroalkyl moieties), and conformational stabilizing moieties, such as those described in commonly owned U.S. Patent Application Publication No. 2007/0059752.Type: ApplicationFiled: July 28, 2011Publication date: November 17, 2011Applicant: Biosearch Technologies, Inc.Inventors: Ronald M. Cook, Matt Lyttle
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Publication number: 20110178280Abstract: The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.Type: ApplicationFiled: August 6, 2010Publication date: July 21, 2011Applicant: BIOSEARCH TECHNOLOGIES, INC.Inventors: RONALD M. COOK, MATT LYTTLE, DAREN DICK
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Publication number: 20110092679Abstract: The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.Type: ApplicationFiled: April 22, 2010Publication date: April 21, 2011Applicant: Biosearch Technologies, Inc.Inventors: Ronald M. Cook, Matt Lyttle, Daren Dick
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Publication number: 20100285052Abstract: Methods and compositions for immediately immunizing an individual against any molecule or compound are provided. The present invention is directed to an immunity linker with at least two sites; (1) at least one first binding site that binds to an immune response component in an individual, and (2) at least one second binding site that binds specifically to a desired compound or molecule, the target. The second binding sites are preferably thiolated aptamers that have the benefit of increased stability, resistance to degradation and longer circulating half life. Methods of making and using pharmaceutical compositions including immunity linker molecules having a thiolated aptamer are also provided.Type: ApplicationFiled: May 5, 2010Publication date: November 11, 2010Inventors: KARY B. MULLIS, JEEVALATHA VIVEKANANDA, JOHNATHAN LLOYD KIEL, RONALD M. COOK
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Publication number: 20100130733Abstract: The present invention provides luminescent metal ion complexes for use in a wide range of biological and chemical studies. The luminescent metal ion complexes of the invention comprise a metal ion chelating component covalently bound to a carrier molecule. Also provided are methods of making and using the luminescent metal ion complexes.Type: ApplicationFiled: December 21, 2009Publication date: May 27, 2010Applicant: BIOSEARCH TECHNOLOGIES, INC.Inventors: RONALD M. COOK, MATT LYTTLE, MARY KATHERINE JOHANNSON
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Publication number: 20100021922Abstract: The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.Type: ApplicationFiled: August 25, 2009Publication date: January 28, 2010Applicant: Biosearch Technologies, Inc.Inventors: Ronald M. Cook, Matt Lyttle, Daren Dick
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Patent number: 7635598Abstract: In one aspect, the invention provides fluorescent probes and assays. The probes include a fluorophore-quencher pair that undergoes a switch from dark to fluorescent in response to a reaction of the quencher. The switch of the probe from dark to fluorescent is typically mediated by an enzyme that acts directly or indirectly on the quencher, interfering with its ability to quench fluorescence emission from the fluorophore. In another aspect, the invention provides a reporter gene assay system and methods of using this system. The assay system includes a fluorophore-quencher probe and an enzyme that acts directly or indirectly on the quencher, increasing the fluorescent emission of the fluorophore. In still other aspects, the invention provides nucleic acid constructs and cells expressing the peptide products of these constructs. In assays of the invention, the presence of a target substance is detected by the switching of fluorescence mediated by the change in oxidation state of the quencher.Type: GrantFiled: July 8, 2005Date of Patent: December 22, 2009Assignee: Biosearch Technologies, Inc.Inventors: Ronald M. Cook, Eliana Saxon Armstrong, Hans E. Johansson
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Patent number: 7635762Abstract: The present invention provides luminescent metal ion complexes for use in a wide range of biological and chemical studies. The luminescent metal ion complexes of the invention comprise a metal ion chelating component covalently bound to a carrier molecule. Also provided are methods of making and using the luminescent metal ion complexes.Type: GrantFiled: October 21, 2003Date of Patent: December 22, 2009Assignee: Biosearch Technologies, Inc.Inventors: Ronald M. Cook, Matt Lyttle, Mary Katherine Johansson
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Publication number: 20090259030Abstract: The present invention provides a new class of solids supports for synthesis of modified oligomers of nucleic acids, and nucleic acid probes that have a format expediently synthesized on the new supports. Exemplary solid supports include at least one quencher bound through a linker to the solid support. Various exemplary embodiments include a moiety that stabilizes a duplex, triplex or higher order aggregation (e.g., hybridization) of nucleic acids of which the oligomer of the invention is a component. Other components of the solid support include moieties that stabilize aggregations of nucleic acids, e.g., intercalators, minor groove binding moieties, bases modified with a stabilizing moiety (e.g., alkynyl moieties, and fluoroalkyl moieties), and conformational stabilizing moieties, such as those described in commonly owned U.S. Patent Application Publication No. 2007/0059752.Type: ApplicationFiled: April 1, 2009Publication date: October 15, 2009Applicant: BIOSEARCH TECHNOLOGIESInventors: Ronald M. Cook, Matt Lyttle
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Patent number: 7582432Abstract: The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.Type: GrantFiled: May 19, 2006Date of Patent: September 1, 2009Assignee: Biosearch Technologies, Inc.Inventors: Ronald M. Cook, Matt Lyttle, Daren Dick
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Patent number: 7160996Abstract: The present invention provides a class of Conformationally Assisted Probes comprising (a) a nucleic acid moiety; (b) an energy donor moiety; (c) an energy acceptor moiety; and (d) one or more stabilizing moieties.Type: GrantFiled: June 8, 2000Date of Patent: January 9, 2007Assignee: Bioresearch Technologies, Inc.Inventor: Ronald M. Cook
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Patent number: 7109312Abstract: The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.Type: GrantFiled: July 29, 2005Date of Patent: September 19, 2006Assignee: Biosearch Technologies, Inc.Inventors: Ronald M. Cook, Matt Lyttle, Daren Dick
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Patent number: 7019129Abstract: The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.Type: GrantFiled: May 9, 2000Date of Patent: March 28, 2006Assignee: Biosearch Technologies, Inc.Inventors: Ronald M. Cook, Matt Lyttle, Daren Dick
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Publication number: 20040146895Abstract: The present invention provides luminescent metal ion complexes for use in a wide range of biological and chemical studies. The luminescent metal ion complexes of the invention comprise a metal ion chelating component covalently bound to a carrier molecule. Also provided are methods of making and using the luminescent metal ion complexes.Type: ApplicationFiled: October 21, 2003Publication date: July 29, 2004Applicant: Biosearch Technologies, Inc.Inventors: Ronald M. Cook, Matt Lyttle, Mary Katherine Johansson
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Patent number: 6761855Abstract: The present invention is directed to an improved column for use in solid phase synthesis or purification. In a specific embodiment, the column is a disposable plastic column for use in solid phase synthesis or purification of complex chemicals, such as biomolecules and more specifically oligonucleotides. In some embodiments, the column has a top orifice with a sufficient diameter so that a fluid line or a multiple fluid line bundle may dispense fluids into the column with great efficiency. The column has an upper cavity portion configured and sized to render it compatible with dispensing pippetors, so that it can be used as a pipette tip or a pipettor can be used to aspirate the column. The column has a lower cavity portion with a shoulder for ready placement of a lower frit to contain the solid support in a central cavity portion of the column. An upper frit can be conveniently placed in the central cavity portion to seal the solid phase resin.Type: GrantFiled: November 5, 2001Date of Patent: July 13, 2004Assignee: Biosearch Technologies, Inc.Inventors: Ronald M. Cook, Rand Dill
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Patent number: 6696298Abstract: Embodiments of the present invention are directed to an improved chemical synthesis apparatus for performing chemical synthesis such as nuclei acid synthesis in a plurality of reaction wells or cells in an efficient manner. The apparatus employs dispenser heads that each include a cluster of nozzles which are fluidicly coupled to a plurality of reagent sources for dispensing different reagents through the single dispenser head. Because each dispenser head is capable of dispensing a plurality of different reagents, the apparatus can perform simultaneous synthesis in a plurality of cells at a high throughput without complex and elaborate control of movement of the dispenser heads relative to the cells.Type: GrantFiled: December 7, 2001Date of Patent: February 24, 2004Assignee: Biosearch Technologies, Inc.Inventors: Ronald M. Cook, Rand Dill, Arnie Baltzley