Patents by Inventor Ronald N. Hines

Ronald N. Hines has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method and cell line for testing cytotoxicity and mutagenicity of a chemical
    Patent number: 5525482
    Abstract: A method of testing the cytotoxicity and mutagenicity of a chemical includes the steps of exposing test cells to the chemical in vitro, intracellularly metabolizing the chemical into a mutagenic or cytotoxic metabolite and then detecting gene/protein/cell damage in the test cells as an indication of the mutagenicity/cytotoxicity of the chemical.A cell line is provided for testing cytotoxicity and mutagenicity of the chemicals, the cell line consisting essentially of fibroblasts normally having no detectable cytochrome P450 mixed function oxidase enzyme activity. The fibroblasts are transformed with chimeric gene constructs containing cytochrome P450 coding sequences and have intracellular cytochrome P450 oxidative metabolizing activity.
    Type: Grant
    Filed: November 15, 1994
    Date of Patent: June 11, 1996
    Assignee: Wayne State University
    Inventors: J. Christopher States, Ronald N. Hines, Raymond F. Novak
  • Method and cell line for testing mutagenicity of a chemical
    Patent number: 5180666
    Abstract: A method of testing the mutagenicity of a chemical includes the steps of exposing test cells to the chemical in vitro, intracellularly metabolizing the chemical into a mutagenic or cytotoxic metabolite and then detecting gene/protein/cell damage in the test cells as an indication of the mutagenicity/cytotoxicity of the chemical.A cell line is provided for testing mutagenicity of the chemicals, the cell line consisting essentially of fibroblasts normally having no detectable cytochrome P450 mixed function oxidase enzyme activity. The fibroblasts are transformed with chimeric gene constructs containing cytochrome P450 coding sequences and have intracellular cytochrome P450 oxidative metaboli zing activity.
    Type: Grant
    Filed: June 27, 1991
    Date of Patent: January 19, 1993
    Assignee: Wayne State University
    Inventors: J. Christopher States, Ronald N. Hines, Raymond F. Novak