Abstract: A plastic roller bottle is provided for suspension cultures for bacteriology or other cell growth or maintenance. The roller bottle comprises a closed bottle defining a longitudinal axis and having flexible plastic walls. Aseptically sealable means for access into the closed body are provided as well. A plurality of spaced reinforcement rings are defined in the flexible plastic walls about the longitudinal axis, to cause the body to normally retain a predetermined, generally cylindrical shape. The flexible plastic walls, apart from the rings, are made of a plastic formulation, and are of a thickness such that the oxygen and carbon dioxide diffusion which takes place through said walls is sufficient to support desired biochemical culturing processes taking place within the roller bottle without venting.