Abstract: A sample holder that can withstand high pressures and temperatures is disclosed. The sample holder can be used to subject a geological sample to the same temperature and pressure that the sample would experience in its native underground environment. The sample holder provides a mechanism for hydrostatically confining the rock core sample to simulate the below ground pressure, while simultaneously allowing fluid to be directed through the core, as needed. The material used for the housing of the sample holder, specifically ceramic zirconia, and the mechanism by which the end fixtures are mounted in the housing, e.g., a square thread or modified square thread, allow for operation at higher pressures and temperatures than is offered by currently available systems. The sample holder can be used to analyze rock core samples via NMR spectroscopy or MRI.

Abstract: The present invention relates to a sample holder that can withstand high pressures and temperatures. In one embodiment, the sample holder of the present invention can be used to subject a geological sample to the same temperature and pressure that the sample would experience in its native underground environment. The present invention provides a mechanism for hydrostatically confining the rock core sample to simulate the below ground pressure, while simultaneously allowing fluid to be directed through the core, as needed. In various embodiments, the material used for the housing of the sample holder, specifically ceramic zirconia, and the mechanism by which the end fixtures are mounted in the housing, e.g., a square thread or modified square thread, allow for operation at higher pressures and temperatures than is offered by currently available systems. In one embodiment, the device of the present invention can be used to analyze rock core samples via NMR spectroscopy or MRI.

Abstract: The methods disclosed herein are useful for achieving higher protein concentrations in reverse micelle solutions by extracting a protein from a metal chelate resin using a reverse micelle solution comprising a polyamine that competitively binds to the metal chelate resin, allowing the protein to elute from the resin. The extracted protein in the reverse micelle solution can then be effectively analyzed, for example using NMR spectroscopy.

Abstract: The methods disclosed herein are useful for achieving higher protein concentrations in reverse micelle solutions by extracting a protein from a metal chelate resin using a reverse micelle solution comprising a polyamine that competitively binds to the metal chelate resin, allowing the protein to elute from the resin. The extracted protein in the reverse micelle solution can then be effectively analyzed, for example using NMR spectroscopy.

Abstract: A general method for solubilizing proteins by direct extraction of an immobilized protein into a reverse micelle solution is disclosed. The reverse micelle protein solutions are amenable to analysis using spectroscopic techniques, particularly NMR spectroscopy.

Abstract: Provided are methods for solubilizing a membrane protein in order to provide substantially homogeneous membrane proteins that are solubilized within reverse micelle systems at concentrations that are suitable for analytical study of such membrane proteins, including nuclear magnetic resonance spectroscopy. Also provided are substantially homogeneous membrane proteins that are solubilized within a reverse micelle system, as well as methods for the study of solubilized membrane proteins, and for the screening of drug candidates that target such membrane proteins.

Abstract: A bolt action hand gun, that is, pistol, having its balance point over the hand. The trigger is in a forward position on the action with an improved connection between the trigger and the sear. The improved connection is such as to prevent accidental firing when the pistol is dropped on the muzzle or butt.