Abstract: An apparatus for sealing an internal environment of a turbomachine includes a first chamber connectable in fluid communication with a high pressure environment of a turbomachine so that a working fluid can flow from the high pressure environment to the first chamber; and a second chamber in fluid communication with a lubrication circuit so that a lubricant can flow from the lubrication circuit to the second chamber. The first and second chambers are arranged in fluid communication with each other so that the working fluid can flow from the first to the second chamber. A return line for the working fluid is in fluid communication with the first chamber and connectable in fluid communication with a low pressure environment of the turbomachine so that the working fluid can flow from the first chamber to the low pressure environment. A pressure regulating device is included along the return line and is configured to provide a predetermined pressure drop.

Abstract: An apparatus for sealing an internal environment of a turbomachine includes a first chamber connectable in fluid communication with a high pressure environment of a turbomachine so that a working fluid can flow from the high pressure environment to the first chamber; and a second chamber in fluid communication with a lubrication circuit so that a lubricant can flow from the lubrication circuit to the second chamber. The first and second chambers are arranged in fluid communication with each other so that the working fluid can flow from the first to the second chamber. A return line for the working fluid is in fluid communication with the first chamber and connectable in fluid communication with a low pressure environment of the turbomachine so that the working fluid can flow from the first chamber to the low pressure environment. A pressure regulating device is included along the return line and is configured to provide a predetermined pressure drop.

Abstract: The present invention relates to a culture including a growth medium and a combination of cytokines consisting of i) interleukin-6 (IL6); ii) flt3-ligand (FLT3); iii) stem cell factor (SCF) and iv) thrombopoi-etin (TPO); the use of the culture for expanding. ex vivo stem cells and/or parental cells and cells differentiated therefrom, and the use of said cells obtainable from said expansion.

Abstract: The present invention relates to a culture including a growth medium and a combination of cytokines consisting of i) interleukin-6 (IL6); ii) flt3-ligand (FLT3); iii) stem cell factor (SCF) and iv) thrombopoi-etin (TPO); the use of the culture for expanding. ex vivo stem cells and/or parental cells and cells differentiated therefrom, and the use of said cells obtainable from said expansion.