Abstract: The present disclosure provides for base editors which satisfy a need in the art for installation of targeted transversions of thymine (T) to adenine (A), or correspondingly, trans versions of adenine (A) to thymine (T). The nucleobase editor domains include a nucleic acid programmable DNA binding protein and an adenosine methyltransferase domain. The base editors may be engineered through the use of continuous or non-continuous evolution systems, such as phage-assisted continuous evolution (PACE). In particular, the present disclosure provides for evolved adenine-to-thymine (or thymine-to-adenine) base editor variants that overcome deficiencies in the art for base editors that can install single-base A:T to T:A transversion mutations. In some embodiments, methods for targeted nucleic acid editing are provided. In some embodiments, pharmaceutical compositions comprising, and vectors and kits for the generation of, targeted base editors are provided.

Abstract: A glove-doll entertainment system is presented. The glove-doll system allows for playing with the doll separate from the glove, playing with the doll attached to the glove, or using the glove as a hand covering without the doll. The appendages of the doll directly and removeably attach to exterior surfaces of the glove's finger receiving cavities. Each of the attached appendages can be separately articulated by finger movement. The doll is three dimensional and is physically distinct from the glove. Contemplated dolls can include an electronic circuit that generates a sound when an appendage is moved. A sequence of sounds can be generated by movement of a plurality of appendages to generate a song or a story. Contemplated gloves are sufficiently thick and waterproof to replace a regular pair of winter gloves.

Abstract: A glove-doll entertainment system is presented. The glove-doll system allows for playing with the doll separate from the glove, playing with the doll attached to the glove, or using the glove as a hand covering without the doll. The appendages of the doll directly and removeably attach to exterior surfaces of the glove's finger receiving cavities. Each of the attached appendages can be separately articulated by finger movement. The doll is three dimensional and is physically distinct from the glove. Contemplated dolls can include an electronic circuit that generates a sound when an appendage is moved. A sequence of sounds can be generated by movement of a plurality of appendages to generate a song or a story. Contemplated gloves are sufficiently thick and waterproof to replace a regular pair of winter gloves.

Abstract: A composition that promotes hair growth includes a lipophilic compound, such as a fatty acid, an allantoin, and a therapeutically effective amount of a biologically active substance. The fatty acid can be a palmitic, palmitoleic, stearic, oleic, linoleic, linolenic, arachidic, eicosenoic, behenic, erucic, myristic acid, and lignoderic fatty acid. The composition also includes jojoba oil, tea tree oil and wild carrot seed oil. A method for promoting hair growth using such composition.