Abstract: An animal restraining apparatus and animal restraining procedure for using the same are provided. The experiment begins by preparing a live experiment animal, such as a mouse or rat coupled to a femoral vein/artery catheter via surgery. The experiment animal in a conscious state is then placed in a restraining apparatus. The restraining apparatus comprises a plurality of partitioning boards to partition the restraining space, such that the animal is restrained in the restraining space to prevent interference and infection from other animal, a supporting structure to carry the experiment animal, a rack for food and water, a signal receiver base, and a tail-fixing component fitted below or above the supporting structure.

Abstract: An animal experiment method is proposed, in which a live test animal is prepared, and at least one tube is intubated into a body of the test animal, wherein one end of the tube comes into contact with body fluid of the test animal, and the other end of the tube is exposed to outside of the test animal. And, a monitoring system is connected to the exposed end of the tube, so as to monitor instant physiological conditions of the test animal in a limited space. Since the test animal stay alive and conscious during entire experiment, no anesthetic is necessarily applied to the test animals, and no undesirable interference caused by anesthetics with animal physiological mechanisms is generated. Therefore, experimental results can truly reflect real physiological and pharmaceutical conditions of the test animals, thereby making experimental data more reliable and significant for use as clinical reference.