Patents by Inventor Ryan C. Blue
Ryan C. Blue has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230272410Abstract: This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.Type: ApplicationFiled: January 20, 2023Publication date: August 31, 2023Applicant: CORTEVA AGRISCIENCE LLCInventors: JOSEPH F. PETOLINO, JIANQUAN LI, STEVEN L. EVANS, RYAN C. BLUE
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Patent number: 11603535Abstract: This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.Type: GrantFiled: October 19, 2020Date of Patent: March 14, 2023Assignee: CORTEVA AGRISCIENCE LLCInventors: Joseph F. Petolino, Jianquan Li, Steven L. Evans, Ryan C. Blue
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Patent number: 11008578Abstract: A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.Type: GrantFiled: December 4, 2018Date of Patent: May 18, 2021Assignee: Corteva Agriscience LLCInventors: W. Michael Ainley, Ryan C. Blue, Michael G. Murray, David Richard Corbin, Rebecca Ruth Miles, Steven R. Webb
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Publication number: 20210087241Abstract: This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.Type: ApplicationFiled: October 19, 2020Publication date: March 25, 2021Applicant: Dow AgroSciences LLCInventors: Joseph F. Petolino, Jianquan Li, Steven L. Evans, Ryan C. Blue
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Patent number: 10836804Abstract: This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.Type: GrantFiled: May 2, 2019Date of Patent: November 17, 2020Assignee: Dow Agrosciences LLCInventors: Joseph Petolino, Jianquan Li, Steven L. Evans, Ryan C. Blue
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Patent number: 10662434Abstract: Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.Type: GrantFiled: November 12, 2015Date of Patent: May 26, 2020Assignees: Dow AgroSciences LLC, Sangamo Therapeutics, Inc.Inventors: Qihua C. Cai, Vipula K. Shukla, Joseph F. Petolino, Lisa W. Baker, Robbi J. Garrison, Ryan C. Blue, Jon C. Mitchell, Nicole L. Arnold, Sarah E. Worden, Jeffrey C. Miller, Fyodor Urnov
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Publication number: 20190330292Abstract: This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.Type: ApplicationFiled: May 2, 2019Publication date: October 31, 2019Inventors: Joseph Petolino, Jianquan Li, Steven L. Evans, Ryan C. Blue
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Patent number: 10351610Abstract: This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.Type: GrantFiled: February 1, 2013Date of Patent: July 16, 2019Assignee: Dow AgroSciences LLCInventors: Joseph Petolino, Jianquan Li, Steven L Evans, Ryan C Blue
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Publication number: 20190085344Abstract: A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.Type: ApplicationFiled: December 4, 2018Publication date: March 21, 2019Inventors: William Michael Ainley, Ryan C. Blue, Michael G. Murray, David Richard Corbin, Rebecca Ruth Miles, Steven R. Webb
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Patent number: 10160975Abstract: A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.Type: GrantFiled: August 12, 2014Date of Patent: December 25, 2018Assignee: Dow AgroSciences LLCInventors: W. Michael Ainley, Ryan C. Blue, Michael G. Murray, David Richard Corbin, Rebecca Ruth Miles, Steven R. Webb
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Patent number: 10077449Abstract: A universal donor polynucleotide is described that can be inserted at targeted locations in plant genomes to facilitate rapid and high throughput integration of a donor molecule within a specific genomic location.Type: GrantFiled: November 3, 2014Date of Patent: September 18, 2018Assignee: Dow AgroSciences LLCInventors: Lakshmi Sastry-Dent, Steven L. Evans, Ryan C. Blue, Zehui Cao
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Publication number: 20160130314Abstract: Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.Type: ApplicationFiled: November 12, 2015Publication date: May 12, 2016Inventors: Qihua C. Cai, Vipula K. Shukla, Joseph F. Petolino, Lisa W. Baker, Robbi J. Garrison, Ryan C. Blue, Jon C. Mitchell, Nicole L. Arnold, Sarah E. Worden, Jeffrey C. Miller, Fyodor Urnov
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Patent number: 9187758Abstract: Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.Type: GrantFiled: December 13, 2007Date of Patent: November 17, 2015Assignees: Sangamo BioSciences, Inc., Dow AgroSciences LLCInventors: Qihua C. Cai, Vipula K. Shukla, Joseph F. Petolino, Lisa W. Baker, Robbi J. Garrison, Ryan C. Blue, Jon C. Mitchell, Nicole L. Arnold, Sarah E. Worden, Jeffrey Miller, Fyodor Urnov
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Publication number: 20150307889Abstract: Disclosed are methods for transformation of an androgenic-derived, haploid cell line with a site-specific nuclease. In some embodiments, the androgenic-derived, haploid cell line is a maize microspore-derived plant tissue culture. In addition, the disclosure provides a method for modifying, e.g., by mutating or targeting and integrating donor DNA into, a specific locus of a haploid or dihaploid tissue genome. The disclosure further provides methods for regenerating a whole plant from the haploid or dihaploid tissue that contains either the mutation at a specific genomic locus or a donor DNA integrated within a specific genomic locus may be obtained from the subject disclosure.Type: ApplicationFiled: April 24, 2015Publication date: October 29, 2015Inventors: Joseph F. Petolino, Tonya L. Strange, Jayakumar Pon Samuel, Ryan C. Blue, Matthew A. Simpson
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Publication number: 20150143588Abstract: A universal donor polynucleotide is described that can be inserted at targeted locations in plant genomes to facilitate rapid and high throughput integration of a donor molecule within a specific genomic location.Type: ApplicationFiled: November 3, 2014Publication date: May 21, 2015Inventors: Lakshmi SASTRY-DENT, Steven L. EVANS, Ryan C. BLUE, Zehui CAO
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Publication number: 20150040267Abstract: A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.Type: ApplicationFiled: August 12, 2014Publication date: February 5, 2015Inventors: William Michael Ainley, Ryan C. Blue, Michael G. Murray, David Richard Corbin, Rebecca Ruth Miles, Steven R. Webb
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Patent number: 8921112Abstract: Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.Type: GrantFiled: October 1, 2008Date of Patent: December 30, 2014Assignees: Dow AgroSciences LLC, Sangamo BioSciences, Inc.Inventors: Qihua C. Cai, Vipula K. Shukla, Joseph F. Petolino, Lisa W. Baker, Robbi J. Garrison, Ryan C. Blue, Jon C. Mitchell, Nicole L. Arnold, Sarah E. Worden, Jeffrey Miller, Fyodor Urnov
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Patent number: 8802921Abstract: A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.Type: GrantFiled: January 21, 2011Date of Patent: August 12, 2014Assignee: Dow AgroSciences, LLC.Inventors: William M. Ainley, Ryan C. Blue, Michael G. Murray, David Corbin, Rebecca R. Miles, Steven R. Webb
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Publication number: 20110191899Abstract: A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.Type: ApplicationFiled: January 21, 2011Publication date: August 4, 2011Applicant: DOW AGROSCIENCES LLCInventors: William M. Ainley, Ryan C. Blue, Michael G. Murray, David Corbin, Rebbeca R. Miles, Steven R. Webb
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Publication number: 20090111188Abstract: Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.Type: ApplicationFiled: October 1, 2008Publication date: April 30, 2009Inventors: Qihua C. Cai, Vipula K. Shukla, Joseph F. Petolino, Lisa W. Baker, Robbi J. Garrison, Ryan C. Blue, Jon C. Mitchell, Nicole L. Arnold, Sarah E. Worden, Jeffrey Miller, Fyodor Urnov