Patents by Inventor Ryan Dregalla
Ryan Dregalla has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240066183Abstract: A wet human or other tissue product may be packaged with a primary and secondary backing. The primary backing may cover a larger area of the tissue than the secondary backing, and the primary backing may be removed first during application of the tissue to a patient, either by sliding or peeling the primary backing away. The secondary backing may assist in holding the tissue in place while the primary backing is removed. The primary backing may provide mechanical support and keep the tissue spread during packaging, handling, and application to a patient. The tissue assembly with primary and secondary backing may be stored in a heat sealed, sterile flat pouch with preservation media. The packaged assembly may include a port for introducing preservation media. A use case for the packaging includes wet, undried amniotic membrane.Type: ApplicationFiled: August 26, 2022Publication date: February 29, 2024Inventor: Ryan Dregalla
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Publication number: 20230250396Abstract: A mesenchymal stem cell harvesting system and method for increasing the efficiency of collecting and processing physiological fluids containing mesenchymal stem cells from a cavity within a patient's skeletal system. Microenvironments risk in MSC production and concentration within a cavity, for example the patient's ilium, are penetrated with a pointed instrument used to create an aperture in the hard cortical bone forming the cavity followed by the insertion of an aspiration device which extracts one or more samples of cancellous bone, bone marrow, bone marrow blood and other aspirated material. The aspirate is rinsed and may be filtered to remove unwanted material and to increase the concentration and purity of the mesenchymal stem cells in the aspirant far beyond levels formerly obtainable for use in autologous treatment of the patient.Type: ApplicationFiled: April 21, 2023Publication date: August 10, 2023Inventors: Edward Jeffrey Donner, Christopher Thomas Donner, Ryan Dregalla, Lucanus Steven Koldewyn
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Publication number: 20230235292Abstract: A mesenchymal stem cell harvesting system and method for increasing the efficiency of collecting and processing physiological fluids containing mesenchymal stem cells from a cavity within a patient’s skeletal system. Microenvironments risk in MSC production and concentration within a cavity, for example the patient’s ilium, are penetrated with a pointed instrument used to create an aperture in the hard cortical bone forming the cavity followed by the insertion of an aspiration device which extracts one or more samples of cancellous bone, bone marrow, bone marrow blood and other aspirated material. The aspirate is rinsed and may be filtered to remove unwanted material and to increase the concentration and purity of the mesenchymal stem cells in the aspirant far beyond levels formerly obtainable for use in autologous treatment of the patient.Type: ApplicationFiled: April 4, 2023Publication date: July 27, 2023Inventors: Edward Jeffrey Donner, Christopher Thomas Donner, Ryan Dregalla, Lucanus Steven Koldewyn
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Patent number: 11655454Abstract: A mesenchymal stem cell harvesting system and method for increasing the efficiency of collecting and processing physiological fluids containing mesenchymal stem cells from a cavity within a patient's skeletal system. Microenvironments risk in MSC production and concentration within a cavity, for example the patient's ilium, are penetrated with a pointed instrument used to create an aperture in the hard cortical bone forming the cavity followed by the insertion of an aspiration device which extracts one or more samples of cancellous bone, bone marrow, bone marrow blood and other aspirated material. The aspirate is rinsed and may be filtered to remove unwanted material and to increase the concentration and purity of the mesenchymal stem cells in the aspirant far beyond levels formerly obtainable for use in autologous treatment of the patient.Type: GrantFiled: March 22, 2018Date of Patent: May 23, 2023Assignee: Elite IP, LLCInventors: Edward Jeffrey Donner, Christopher Thomas Donner, Ryan Dregalla, Lucanus Steven Koldewyn
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Publication number: 20220184135Abstract: The embodiments disclosed herein generally relate to systems, devices and methods for the fractionation, isolation, extraction and processing of the adipose supernatant layer of a bone marrow aspirate. In particular, the various embodiments relate to systems, devices, and methods of obtaining, utilizing, and processing the adipose supernatant layer of a bone marrow aspirate as a source of mesenchymal stem cells.Type: ApplicationFiled: January 6, 2022Publication date: June 16, 2022Inventors: Christopher Centeno, Ryan Dregalla, Nicolette Lyons, Patrick Reischling
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Patent number: 11253548Abstract: A method of producing the constituents of a therapeutic product from mammalian cells is described herein. Cells are isolated from a mammalian source. The cells are exposed to supercritical carbon dioxide (SCCO2) for 1 to 30 minutes, where the SCCO2 is maintained at a pressure of 1071 pounds per square inch (PSI) and a temperature of 31.1 to 45 degrees Celsius during the exposure. The exposure dissociates the cellular membranes of the cells to release intramembrane components therein to produce constituents of the therapeutic product. The mammalian cells may include at least one of platelets, stem cells, germ cells, and somatic cells. The methods described herein are particularly advantageous for releasing and capturing therapeutic intramembrane components from platelets and alpha-granules.Type: GrantFiled: March 11, 2019Date of Patent: February 22, 2022Assignee: Napa Medical Research FoundationInventors: Ryan Dregalla, Marko Bodor, Yvette Uribe
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SYSTEMS AND METHODS FOR COLLECTING, PROCESSING, OPTIMIZING, AND PREPARING STEM CELLS FOR FURTHER USE
Publication number: 20210080358Abstract: A system for collecting and processing a sample of an enriched population of mesenchymal stem cells in a matrix of body tissue in which the sample is separated into smaller segments to optimize the ability of the system to organize the stem cells into small working clusters with tissue. The tissue clusters are separated into optimized groupings for further processing and use in medical procedures.Type: ApplicationFiled: August 28, 2020Publication date: March 18, 2021Applicant: Elite IP, LLCInventors: Edward Jeffrey Donner, Lucanus Steven Koldewyn, Christopher Thomas Donner, Ryan Dregalla, Kristopher Buchanan, William Buchanan, Robert Mitchell Clark -
Publication number: 20200199512Abstract: An acellular product may be derived from human placenta and may be used in various scenarios for wound healing. Because the product may be acellular, the product may be processed for storage and transportation with minimal degradation. The product may include various scaffolding such as biomaterials or human tissue, and the scaffolding may be infused with various plasmas and agents. The cell-free treatment may maintain the biological activity of many therapeutic agents found within cells and may possess multiple structural components to support cellular attachment. The structural components or scaffolds may function as a reservoir of highly diffusible chemotactic and cellular-programming factors that may be useful to treat injury and disease. In many cases, fibrinogen may be absent, which may reduce scarring.Type: ApplicationFiled: February 18, 2020Publication date: June 25, 2020Inventor: Ryan Dregalla
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Publication number: 20200129560Abstract: The embodiments disclosed herein generally relate to systems, devices and methods for the fractionation, isolation, extraction and processing of the adipose supernatant layer of a bone marrow aspirate. In particular, the various embodiments relate to systems, devices, and methods of obtaining, utilizing, and processing the adipose supernatant layer of a bone marrow aspirate as a source of mesenchymal stem cells.Type: ApplicationFiled: December 30, 2019Publication date: April 30, 2020Inventors: Christopher Centeno, Ryan Dregalla, Nicolette Lyons, Patrick Reischling
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Patent number: 10537596Abstract: The embodiments disclosed herein generally relate to systems, devices and methods for the fractionation, isolation, extraction and processing of the adipose supernatant layer of a bone marrow aspirate. In particular, the various embodiments relate to systems devices and methods of obtaining, utilizing and processing the adipose supernatant layer of a bone marrow aspirate as a source of mesenchymal stem cells.Type: GrantFiled: April 20, 2018Date of Patent: January 21, 2020Assignee: Regenexx LLCInventors: Christopher Centeno, Ryan Dregalla, Nicolette Lyons, Patrick Reischling
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Publication number: 20190025162Abstract: Apparatus, systems and methods for processing a blood sample. One embodiment includes an isolation container having at least one sidewall defining an interior volume. The interior volume includes a medial reservoir in fluid communication with proximal and distal reservoirs. The diameter of the medial reservoir is less than the diameter of the proximal and distal reservoirs. Therefore, the isolation container has an interior volume which is roughly hour-glass shaped with the medial reservoir being a substantially narrowed portion or channel between two wider portions. The isolation container is configured such that the buffy coat layer of fractionated blood will be located within, or may be manipulated to be with the medial reservoir.Type: ApplicationFiled: September 20, 2018Publication date: January 24, 2019Inventors: Christopher J. Centeno, Brian Leach, Ryan Dregalla, Patrick Reischling
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Publication number: 20180325962Abstract: An acellular product may be derived from human placenta and may be used in various scenarios for wound healing. Because the product may be acellular, the product may be processed for storage and transportation with minimal degradation. The product may include various scaffolding such as biomaterials or human tissue, and the scaffolding may be infused with various plasmas and agents. The cell-free treatment may maintain the biological activity of many therapeutic agents found within cells and may possess multiple structural components to support cellular attachment. The structural components or scaffolds may function as a reservoir of highly diffusible chemotactic and cellular-programming factors that may be useful to treat injury and disease.Type: ApplicationFiled: July 24, 2018Publication date: November 15, 2018Inventors: Ryan Dregalla, Leonard R. Duford, Dean Nistal, Tracee Gruber
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Publication number: 20180325961Abstract: An acellular product may be derived from human placenta and may be used in various scenarios for wound healing. Because the product may be acellular, the product may be processed for storage and transportation with minimal degradation. The product may include various scaffolding such as biomaterials or human tissue, and the scaffolding may be infused with various plasmas and agents. The cell-free treatment may maintain the biological activity of many therapeutic agents found within cells and may possess multiple structural components to support cellular attachment. The structural components or scaffolds may function as a reservoir of highly diffusible chemotactic and cellular-programming factors that may be useful to treat injury and disease.Type: ApplicationFiled: July 24, 2018Publication date: November 15, 2018Inventors: Ryan Dregalla, Leonard R. Duford, Dean Nistal, Tracee Gruber
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Publication number: 20180305655Abstract: The embodiments disclosed herein generally relate to systems, devices and methods for the fractionation, isolation, extraction and processing of the adipose supernatant layer of a bone marrow aspirate. In particular, the various embodiments relate to systems devices and methods of obtaining, utilizing and processing the adipose supernatant layer of a bone marrow aspirate as a source of mesenchymal stem cells.Type: ApplicationFiled: April 20, 2018Publication date: October 25, 2018Inventors: Christopher Centeno, Ryan Dregalla, Nicolette Lyons, Patrick Reischling
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Patent number: 10088391Abstract: Apparatus, systems and methods for processing a blood sample. One embodiment comprises an isolation container having at least one sidewall defining an interior volume. The interior volume includes a medial reservoir in fluid communication with proximal and distal reservoirs. The diameter of the medial reservoir is less than the diameter of the proximal and distal reservoirs. Therefore, the isolation container has an interior volume which is roughly hour-glass shaped with the medial reservoir being a substantially narrowed portion or channel between two wider portions. The isolation container is configured such that the buffy coat layer of fractionated blood will be located within and may be accessed from the reduced cross sectional medial reservoir after a centrifuge or other processing step.Type: GrantFiled: February 18, 2014Date of Patent: October 2, 2018Assignee: Regenexx, LLCInventors: Christopher J. Centeno, Brian Leach, Ryan Dregalla, Patrick Reischling
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Patent number: 9976115Abstract: The embodiments disclosed herein generally relate to systems, devices and methods for the fractionation, isolation, extraction and processing of the adipose supernatant layer of a bone marrow aspirate. In particular, the various embodiments relate to systems devices and methods of obtaining, utilizing and processing the adipose supernatant layer of a bone marrow aspirate as a source of mesenchymal stem cells.Type: GrantFiled: August 6, 2014Date of Patent: May 22, 2018Assignee: Regenexx, LLCInventors: Christopher Centeno, Ryan Dregalla, Nicolette Lyons, Patrick Reischling
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Publication number: 20170136070Abstract: An acellular product may be derived from human placenta and may be used in various scenarios for wound healing. Because the product may be acellular, the product may be processed for storage and transportation with minimal degradation. The product may include various scaffolding such as biomaterials or human tissue, and the scaffolding may be infused with various plasmas and agents. The cell-free treatment may maintain the biological activity of many therapeutic agents found within cells and may possess multiple structural components to support cellular attachment. The structural components or scaffolds may function as a reservoir of highly diffusible chemotactic and cellular-programming factors that may be useful to treat injury and disease. In many cases, fibrinogen may be absent, which may reduce scarring.Type: ApplicationFiled: November 18, 2015Publication date: May 18, 2017Inventor: Ryan Dregalla
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Publication number: 20160298076Abstract: The embodiments disclosed herein generally relate to systems, devices and methods for the fractionation, isolation, extraction and processing of the adipose supernatant layer of a bone marrow aspirate. In particular, the various embodiments relate to systems devices and methods of obtaining, utilizing and processing the adipose supernatant layer of a bone marrow aspirate as a source of mesenchymal stem cells.Type: ApplicationFiled: August 6, 2014Publication date: October 13, 2016Inventors: Christopher Centeno, Ryan Dregalla, Nicolette Lyons, Patrick Reischling
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Publication number: 20160003712Abstract: Apparatus, systems and methods for processing a blood sample. One embodiment comprises an isolation container having at least one sidewall defining an interior volume. The interior volume includes a medial reservoir in fluid communication with proximal and distal reservoirs. The diameter of the medial reservoir is less than the diameter of the proximal and distal reservoirs. Therefore, the isolation container has an interior volume which is roughly hour-glass shaped with the medial reservoir being a substantially narrowed portion or channel between two wider portions. The isolation container is configured such that the buffy coat layer of fractionated blood will be located within and may be accessed from the reduced cross sectional medial reservoir after a centrifuge or other processing step.Type: ApplicationFiled: February 18, 2014Publication date: January 7, 2016Inventors: Christopher J. Centeno, Brian Leach, Ryan Dregalla, Patrick Reischling
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Publication number: 20160002598Abstract: Device, system and method embodiments are disclosed herein which provide for the production of a modified autologous platelet solution at the patient bedside for contemporaneous reinjection to the patient. In certain embodiments all of the steps including, but not limited to blood draw, platelet lysis, solution preparation and reinjection to a patient may be accomplished in a single office or clinic visit without relocating the patient. Accordingly, the apparatus, devices and systems disclosed herein generally include a substantially stand-alone machine, device or system which is configured to accept a platelet containing solution, induce lysis of one or more platelet bodies within the platelet containing solution and provide the resulting modified platelet solution in a manner suitable for injection into the patient.Type: ApplicationFiled: January 28, 2014Publication date: January 7, 2016Applicant: Regenerative Science, LLCInventors: Christopher J. Centeno, Brian J. Leach, Ryan Dregalla, Patrick Reischling, Nicolette Lyons