Patents by Inventor Ryoji Inaba
Ryoji Inaba has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8366896Abstract: Provided is a capillary electrophoresis device including a holder preventing a septum from coming off when a capillary is pulled out, and also allowing containers to be taken out in any order. In the capillary electrophoresis device for separating and analyzing a sample such as a DNA and a protein by electrophoresis, the holder includes: a septum having a capillary hole through which a capillary penetrates; a container for storing a solution; and a container-accommodation unit for accommodating the container. A hole formed in the septum engages with an engagement portion formed on the container, and thereby the septum is held to cover the container.Type: GrantFiled: May 30, 2008Date of Patent: February 5, 2013Assignee: Hitachi High-Technologies CorporationInventors: Takeshi Ooura, Ryoji Inaba
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Patent number: 8349161Abstract: Bubbles can be removed regardless of an individual difference of a pump to fill an electrophoresis medium into a capillary. Of flow passages formed between an inner side surface of a container for accommodating the electrophoresis medium and a side surface of a plunger, one of the flow passages causing an electrophoresis medium to be easily stagnant is formed to have the cross-sectional area larger than the cross-sectional area of the other flow passage on the opposite side. In other words, the flow passage portion causing the electrophoresis medium to be easily stagnant is formed in such a manner as to increase a flow amount of the electrophoresis medium. This can eliminate a region having an extremely small amount of electrophoresis medium flow in the pump.Type: GrantFiled: April 6, 2010Date of Patent: January 8, 2013Assignee: Hitachi High-Technologies CorporationInventors: Takashi Gomi, Ryoji Inaba, Taro Nakazawa, Takeshi Ohura, Mari Kotoura
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Publication number: 20120329143Abstract: A chilled reagent container comprises a reagent vessel containing part for containing therein a plurality of reagent vessels, a container lid including a container lid hole through which the reagent vessels contained by the reagent vessel containing part are accessible, and a cooling block for cooling the reagent vessels contained by the reagent vessel containing part, wherein the container lid slides to be changeable between an opened situation wherein the reagent is accessible from an outside and a closed situation wherein the reagent is prevented from being accessed from the outside, wherein the chilled reagent container further comprises a reagent container packing including another hole through which the reagent vessels are accessible and arranged between the container lid and the reagent vessel containing part to be pressed against the container lid.Type: ApplicationFiled: June 25, 2012Publication date: December 27, 2012Applicant: Hitachi High-Technologies CorporationInventors: Motohiro Yamazaki, Ryoji Inaba, Shuhei Yamamoto, Takuya Matsui, Kohshi Maeda, Yuichiro Ota, Hiroyuki Higashino
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Publication number: 20120316087Abstract: Provided is a reaction device for nucleic acid analysis wherein microparticles, which carry a nucleic acid to be detected having been immobilized thereon, are aligned in a lattice form on a substrate according to the pixel size of a two-dimensional sensor. By this reaction device for nucleic acid analysis which is provided with a channel-forming reaction chamber on the substrate (101), the nucleic acid having been immobilized on the microparticles (103) on the substrate (101) is detected. The microparticles (103), which carry the nucleic acid to be detected having been immobilized thereon, are arranged by microstructures (102) aligned on the substrate (101).Type: ApplicationFiled: December 1, 2010Publication date: December 13, 2012Applicant: Hitachi High-Technologies CorporationInventors: Yoshiaki Sugimura, Masatoshi Narahara, Kazumichi Imai, Toshiro Saito, Ryoji Inaba, Takuya Matsui
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Publication number: 20120292189Abstract: The invention provides a capillary electrophoresis apparatus which can improve the operability and measuring speed. According to the invention, a sensor for identifying the type of sample containers is fixed at the position away from a capillary anode electrode. The sensor is made to be closer to the sample containers by moving a moving stage so that the sample containers disposed on the moving stage can be identified by the sensor. A fixing apparatus for fixing at least a pair of sample containers is provided on the moving stage.Type: ApplicationFiled: July 26, 2012Publication date: November 22, 2012Applicant: Hitachi High-Technologies CorporationInventors: Takeshi OHURA, Ryoji Inaba, Yoshitaka Kodama, Hiromi Yamashita, Manabu Akiba, Tomohiro Shoji
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Publication number: 20120248334Abstract: System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light.Type: ApplicationFiled: December 28, 2011Publication date: October 4, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Richard T. Reel, Eric S. Nordman, Tomoyuki Sakai, Satoshi Takahashi, Ryoji Inaba
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Patent number: 8262888Abstract: There is provided a capillary electrophoresis apparatus wherein coupling efficiency of exciting irradiation light to a capillary array does not decline even if any one capillary array of two capillary arrays is removed. Light emission generated by capillaries of each capillary array of 2n (n is a positive integer) capillary arrays radiating exciting light from one side is detected by one optical detection system.Type: GrantFiled: March 4, 2008Date of Patent: September 11, 2012Assignee: Hitachi High-Technologies CorporationInventors: Ryoji Inaba, Motohiro Yamazaki, Tomohiro Shoji, Takeshi Ohura, Takashi Gomi
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Patent number: 8257569Abstract: In a capillary electrophoresis apparatus, a capillary array unit has a capillary array including capillaries having a capillary head and a detection unit, a frame for supporting the capillary array, and a load header for holding cathode ends of the capillaries. The frame has separators for separating and holding the capillaries. The capillary head, the detection unit, and the separators are disposed along one linear line. With this arrangement, there is provided the capillary array unit and the capillary electrophoresis apparatus which are arranged such that a job for mounting the capillary array can be executed easily.Type: GrantFiled: January 12, 2009Date of Patent: September 4, 2012Assignee: Hitachi High-Technologies CorporationInventors: Tomohiro Shoji, Toshiyuki Sakurai, Takashi Gomi, Ryoji Inaba
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Patent number: 8252162Abstract: The invention provides a capillary electrophoresis apparatus which can improve the operability and measuring speed. According to the invention, a sensor for identifying the type of sample containers is fixed at the position away from a capillary anode electrode. The sensor is made to be closer to the sample containers by moving a moving stage so that the sample containers disposed on the moving stage can be identified by the sensor. A fixing apparatus for fixing at least a pair of sample containers is provided on the moving stage.Type: GrantFiled: December 24, 2008Date of Patent: August 28, 2012Assignee: Hitachi High-Technologies CorporationInventors: Takeshi Ohura, Ryoji Inaba, Yoshitaka Kodama, Hiromi Yamashita, Manabu Akiba, Tomohiro Shoji
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Patent number: 8246806Abstract: An electrophoresis apparatus includes a multi-capillary array having a liquid or solid disposed between the capillaries of the array. The liquid or solid exhibits a refractive index higher than that of air and less than that of water and reduces the amount of laser beams scattered by the capillaries. Also provided are methods of adjusting refracted and reflected excitation light beams passing through capillaries of a multi-capillary array, to reduce loss of intensity of the laser beams and increase irradiation of respective samples disposed in the capillaries.Type: GrantFiled: March 6, 2007Date of Patent: August 21, 2012Assignees: Applera Corporation, Hitachi, Ltd.Inventors: Ryoji Inaba, Tomohiro Shoji, Shozo Kasai, Masaya Kojima, Yasushi Shimizu, Seiichi Ugai, Eric S. Nordman
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Patent number: 8246803Abstract: An object of the present invention is to provide a capillary electrophoresis apparatus in which simultaneity can be ensured between sensitivity and data acquisition to decrease a pull-up signal while spectral data acquisition is eliminated in each capillary exchange. The invention relates to a capillary electrophoresis apparatus characterized in that a multi-bandpass filter is provided in an optical detection system. In one aspect of the invention, a signal detection area of a two-dimensional detector is divided into plural regions corresponding to wavelength transmission regions of the multi-bandpass filter. An integrated value of the fluorescence spectrum signal is determined in the region including a fluorescence spectrum peak of an analysis sample in the plural regions. The analysis is performed with the integrated value.Type: GrantFiled: January 18, 2007Date of Patent: August 21, 2012Assignee: Hitachi High Technologies CorporationInventors: Motohiro Yamazaki, Ryoji Inaba, Satoshi Takahashi, Shigeru Matsui
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Publication number: 20120126142Abstract: Disclosed is a fluorescent analysis method whereby the throughput in DNA sequence analysis or the like can be improved. The method comprises irradiating a substrate, which carries biological molecules such as oligonucleotides immobilized thereon, with light for fluorescent measuring, collecting the generated fluorescence, dispersing the collected light, forming an image by focusing the light on a two-dimensional sensor, and then detecting the fluorescence with the two-dimensional sensor. In this method, since wavelengths are dispersed in different directions and then detected at the same time, the intensity of each dispersed wavelength and the position of the subject of spectroscopic imaging can be calculated even in the case where the wavelength dispersion distance is longer than the inter-lattice distance.Type: ApplicationFiled: April 14, 2010Publication date: May 24, 2012Inventors: Takuya Matsui, Ryoji Inaba
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Patent number: 8142635Abstract: A capillary array includes a light detection portion, a sample supply portion, a buffer solution supply portion and a voltage application portion which are necessary functions for electrophoresis, thereby, when assembling the capillary array into an electrophoresis apparatus, the same can be immediately used. Accordingly, a capillary array is provided which can be easily incorporated into an electrophoresis apparatus.Type: GrantFiled: July 26, 2010Date of Patent: March 27, 2012Assignee: Hitachi, Ltd.Inventors: Yasushi Shimizu, Toshiaki Kita, Kiyoshi Tsukada, Akihiro Suzuki, Tomonari Morioka, Masaya Kojima, Ryoji Inaba, Daizo Tokinaga, Shuhei Yamamoto, Yoshiyuki Okishima, Masao Fukunaga, Takayasu Furukawa, Tomohiro Shoji
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Publication number: 20120031762Abstract: Bubbles can be removed regardless of an individual difference of a pump to fill an electrophoresis medium into a capillary. Of flow passages formed between an inner side surface of a container for accommodating the electrophoresis medium and a side surface of a plunger, one of the flow passages causing an electrophoresis medium to be easily stagnant is formed to have the cross-sectional area larger than the cross-sectional area of the other flow passage on the opposite side. In other words, the flow passage portion causing the electrophoresis medium to be easily stagnant is formed in such a manner as to increase a flow amount of the electrophoresis medium. This can eliminate a region having an extremely small amount of electrophoresis medium flow in the pump.Type: ApplicationFiled: April 6, 2010Publication date: February 9, 2012Inventors: Takashi Gomi, Ryoji Inaba, Taro Nakazawa, Takeshi Ohura, Mari Kotoura
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Publication number: 20120024707Abstract: The solution reservoir apparatus of the capillary electrophoresis apparatus securely affixes an evaporation-preventing membrane to a container when a capillary is inserted or withdrawn, without extending the cathode end of the capillary. The solution reservoir apparatus comprises a container for reserving a sample or solution, a cover having a bore through which the capillary is passed and covering the container, an evaporation-preventing membrane having a capillary hole through which the capillary is passed, and a container holder for holding the container. The evaporation-preventing membrane has a projection provided at the periphery of the capillary hole, the projection of the evaporation-preventing membrane is engaged with the bore on the cover when the evaporation-preventing membrane is positioned on the cover, and the evaporation-preventing membrane is supported by the cover.Type: ApplicationFiled: April 8, 2010Publication date: February 2, 2012Inventors: Takeshi Ohura, Ryoji Inaba, Manabu Akiba, Takashi Gomi, Mari Kotoura
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Patent number: 8102529Abstract: System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light.Type: GrantFiled: December 14, 2009Date of Patent: January 24, 2012Assignees: Applied Biosystems, LLC, Hitachi High-Technologies CorporationInventors: Richard Reel, Eric Nordman, Tomoyuki Sakai, Satoshi Takahashi, Ryoji Inaba
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Patent number: 8062592Abstract: Even if a standby time to the measurement becomes long, a sample in a sample container is prevented from deteriorating. A cooling mechanism is provided in a sample tray for holding a sample plate, or in a plate-mounting portion of a table-type auto sampler of a capillary electrophoresis apparatus, so that the sample plate is cooled during the measurement also.Type: GrantFiled: July 18, 2008Date of Patent: November 22, 2011Assignee: Hitachi High-Technologies CorporationInventors: Miho Ozawa, Ryoji Inaba, Tomohiro Shoji, Manabu Akiba
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Patent number: 8043493Abstract: An electrophoresis apparatus includes a multi-capillary array having a liquid or solid disposed between the capillaries of the array. The liquid or solid exhibits a refractive index higher than that of air and less than that of water and reduces the amount of laser beams scattered by the capillaries. Also provided are methods of adjusting refracted and reflected excitation light beams passing through capillaries of a multi-capillary array, to reduce loss of intensity of the laser beams and increase irradiation of respective samples disposed in the capillaries.Type: GrantFiled: March 6, 2007Date of Patent: October 25, 2011Assignees: Hitachi, Ltd., Applera CorporationInventors: Ryoji Inaba, Tomohiro Shoji, Shozo Kasai, Masaya Kojima, Yasushi Shimizu, Seiichi Ugai
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Patent number: 8012327Abstract: An object of the present invention is to provide a capillary electrophoresis apparatus in which simultaneity can be ensured between sensitivity and data acquisition to decrease a pull-up signal while spectral data acquisition is eliminated in each capillary exchange. The invention relates to a capillary electrophoresis apparatus in which capillary position shift is detected in each capillary exchange by detecting a capillary position. A capillary position measuring light source is provided in the capillary electrophoresis apparatus of the invention. The capillary is irradiated with light emitted from the capillary position measuring light source, a capillary image is detected with a two-dimensional detector, and thereby a position deviation of the capillary is determined. On the basis of the position deviation of the capillary, a data acquisition area is set in the two-dimensional detector, or a reference fluorescent light spectrum determined from the capillary at the standard position is corrected.Type: GrantFiled: January 16, 2007Date of Patent: September 6, 2011Assignee: Hitachi High-Technologies CorporationInventors: Motohiro Yamazaki, Ryoji Inaba, Satoshi Takahashi, Isao Haraura, Tomoyuki Sakai
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Patent number: 7943027Abstract: The object of the present invention is that a dynamic range is extended in an electrophoresis unit and concentration differences among a plurality of samples measured simultaneously are increased. An irradiation time to the samples is adjusted during analysis without changing a sampling time. By shortening the irradiation time, a fluorescence amount of the samples is reduced to cause signal intensity detected by a detector to physically decrease. If the irradiation time is very short (several 100 msec), the irradiation time and fluorescence intensity are in a direct proportional relationship. It is known that, if the irradiation time is reduced to 1/n, the fluorescence intensity, that is, signal intensity to be detected will be 1/n. Thus, for data whose irradiation time is reduced to 1/n during analysis, data obtained by multiplying a substantially measured value by n is used for data analysis as a true value to be originally acquired.Type: GrantFiled: November 6, 2007Date of Patent: May 17, 2011Assignee: Hitachi High-Technologies CorporationInventors: Motohiro Yamazaki, Ryoji Inaba, Satoshi Takahashi, Tomohiro Shoji, Takeshi Ohura, Takashi Gomi