Abstract: A method for detecting a genetic mutation is provided. The method comprising: carrying out PCR using a template DNA, a forward primer, a reverse primer and a wild-type oligonucleotide; and detecting an amplified DNA. In the method, the wild-type oligonucleotide comprises LNA or BNA, and on a DNA strand of the template, with which the wild-type oligonucleotide is hybridized, a region with which the wild-type oligonucleotide is a hybridized and a region with which the forward primer or the reverse primer is hybridized partially overlap each other, or the regions are separated from each other by 1 to 18 bases.

Abstract: A genetic detection and determination apparatus which detects or determines genetic information, provided with a reaction container having a reaction vessel, including: a container set portion on which the reaction container is set; a moving mechanism which moves the container set portion along a track; a reaction vessel dividing portion which divides the reaction vessel into a plurality of reaction chambers by deforming the reaction vessel; a heating and cooling portion which heats and cools the reaction container; and a measuring portion which is movable above the container set portion in parallel to an upper surface of the reaction container provided on the container set portion and which measures a reaction within each of the reaction chambers.

Abstract: The reaction chip of the present invention has a plurality of recesses 6 constituting a part of a reaction container and a groove constituting a part of a channel formed on at least one of one face of a first base material (resin base material 2) and one face of a second base material (metallic base material) and a notch 15 showing a gradual increase in width and a gradual increase in depth from one face 2d of the base material toward an inner wall surface 6d of the recess is formed on an edge of at least one recess in an extending direction of the groove. One face of the first base material and one face of the second base material are stuck together opposite to each other to form the plurality of reaction containers and the channel.

Abstract: The reaction chip of the present invention has a plurality of recesses 6 constituting a part of a reaction container and a groove constituting a part of a channel formed on at least one of one face of a first base material (resin base material 2) and one face of a second base material (metallic base material) and a notch 15 showing a gradual increase in width and a gradual increase in depth from one face 2d of the base material toward an inner wall surface 6d of the recess is formed on an edge of at least one recess in an extending direction of the groove. One face of the first base material and one face of the second base material are stuck together opposite to each other to form the plurality of reaction containers and the channel.

Abstract: The reaction chip of the present invention has a plurality of recesses 6 constituting a part of a reaction container and a groove constituting a part of a channel formed on at least one of one face of a first base material (resin base material 2) and one face of a second base material (metallic base material) and a notch 15 showing a gradual increase in width and a gradual increase in depth from one face 2d of the base material toward an inner wall surface 6d of the recess is formed on an edge of at least one recess in an extending direction of the groove. One face of the first base material and one face of the second base material are stuck together opposite to each other to form the plurality of reaction containers and the channel.

Abstract: A genetic detection and determination apparatus which detects or determines genetic information, provided with a reaction container having a reaction vessel, including: a container set portion on which the reaction container is set; a moving mechanism which moves the container set portion along a track; a reaction vessel dividing portion which divides the reaction vessel into a plurality of reaction chambers by deforming the reaction vessel; a heating and cooling portion which heats and cools the reaction container; and a measuring portion which is movable above the container set portion in parallel to an upper surface of the reaction container provided on the container set portion and which measures a reaction within each of the reaction chambers.

Abstract: The reaction chip of the present invention has a plurality of recesses 6 constituting a part of a reaction container and a groove constituting a part of a channel formed on at least one of one face of a first base material (resin base material 2) and one face of a second base material (metallic base material) and a notch 15 showing a gradual increase in width and a gradual increase in depth from one face 2d of the base material toward an inner wall surface 6d of the recess is formed on an edge of at least one recess in an extending direction of the groove. One face of the first base material and one face of the second base material are stuck together opposite to each other to form the plurality of reaction containers and the channel.

Abstract: A reaction vessel suitable for automating of various measurements is provided. In a preferred embodiment, on the same side of plate-like substrate (10), sample injection part (12), typing reagent reservoir part (14) and mineral oil reservoir part (16) are provided as concaves, and further, multiple probe arrangement parts (18) are provided. The typing reagent reservoir part (14) and mineral oil reservoir part (16) are sealed with film (20). The surface of the substrate (10) is covered with detachable sealing material (22) with a size capable of covering the sample injection part (12), typing reagent reservoir part (14), mineral oil reservoir part (16) and multiple probe arrangement parts (18) in such a manner that the film (20) is covered by the sealing material (22). Liquid transfer is carried out through nozzles.