Patents by Inventor Ryota Iino
Ryota Iino has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10809257Abstract: This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41?) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41?) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).Type: GrantFiled: January 29, 2019Date of Patent: October 20, 2020Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Hiroyuki Noji, Ryota Iino, Suguru Araki
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Publication number: 20190154682Abstract: This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41?) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41?) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).Type: ApplicationFiled: January 29, 2019Publication date: May 23, 2019Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Hiroyuki NOJI, Ryota IINO, Suguru ARAKI
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Patent number: 10267793Abstract: This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41?) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41?) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).Type: GrantFiled: November 15, 2016Date of Patent: April 23, 2019Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Hiroyuki Noji, Ryota Iino, Suguru Araki
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Patent number: 9797837Abstract: There are provided a detector and a detection method capable of detecting a biological sample with high sensitivity. A detector is provided with a microchamber array including a plurality of storage sections to be filled with a hydrophilic solvent containing a biological sample; and an image sensor in which picture elements are disposed corresponding to the storage sections, wherein the microchamber array includes a flow channel in communication with openings of the storage sections; a hydrophobic solvent supply unit arranged in continuity with the flow channel; and a through-hole which allows the hydrophilic solvent to enter and exit the flow channel, and the hydrophobic solvent supply unit flows a hydrophobic solvent into the flow channel by an externally-applied force.Type: GrantFiled: August 29, 2013Date of Patent: October 24, 2017Assignee: The University of TokyoInventors: Soo Hyeon Kim, Hiroyuki Noji, Ryota Iino, Jun Ohta, Takashi Tokuda, Kiyotaka Sasagawa, Toshihiko Noda
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Publication number: 20170115284Abstract: This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41?) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41?) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).Type: ApplicationFiled: November 15, 2016Publication date: April 27, 2017Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Hiroyuki NOJI, Ryota IINO, Suguru ARAKI
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Publication number: 20160223531Abstract: This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41?) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41?) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).Type: ApplicationFiled: March 28, 2016Publication date: August 4, 2016Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Hiroyuki NOJI, Ryota IINO, Suguru ARAKI
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Patent number: 9399788Abstract: The present disclosure describes a method capable of easily and rapidly inspecting the susceptibility of bacteria or fungi to an antimicrobial drug. The inspection method of the present disclosure is a method for inspecting susceptibility of bacteria or fungi to an antimicrobial drug using a micro-device having flow channels, including: incubating a mixture of the antimicrobial drug and a suspension to be inspected in the flow channels of the micro-device; and detecting bacteria or fungi derived from the suspension to be inspected in an observation area of the flow channels of the micro-device. The detecting step can be performed by detecting an increase or decrease in the number of or a change in shape of bacteria or fungi derived from the suspension to be inspected in the observation area by a microscope or the like.Type: GrantFiled: August 31, 2012Date of Patent: July 26, 2016Assignees: Osaka University, FUKOKU Co., Ltd.Inventors: Yoshimi Matsumoto, Kohei Hayama, Shouichi Sakakihara, Kunihiko Nishino, Akihito Yamaguchi, Hiroyuki Noji, Ryota Iino
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Patent number: 9329174Abstract: This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41?) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41?) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).Type: GrantFiled: March 7, 2012Date of Patent: May 3, 2016Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Hiroyuki Noji, Ryota Iino, Suguru Araki
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Publication number: 20150204785Abstract: There are provided a detector and a detection method capable of detecting a biological sample with high sensitivity. A detector is provided with a microchamber array including a plurality of storage sections to be filled with a hydrophilic solvent containing a biological sample; and an image sensor in which picture elements are disposed corresponding to the storage sections, wherein the microchamber array includes a flow channel in communication with openings of the storage sections; a hydrophobic solvent supply unit arranged in continuity with the flow channel; and a through-hole which allows the hydrophilic solvent to enter and exit the flow channel, and the hydrophobic solvent supply unit flows a hydrophobic solvent into the flow channel by an externally-applied force.Type: ApplicationFiled: August 29, 2013Publication date: July 23, 2015Applicant: THE UNIVERSITY OF TOKYOInventors: Soo Hyeon Kim, Hiroyuki Noji, Ryota Iino, Jun Ohta, Takashi Tokuda, Kiyotaka Sasagawa, Toshihiko Noda
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Publication number: 20140349333Abstract: The present invention provides a novel method capable of easily and rapidly inspecting the susceptibility of bacteria or fungi to an antimicrobial drug and an inspection system for use in the method. The inspection method of the present invention is a method for inspecting susceptibility of bacteria or fungi to an antimicrobial drug using a micro-device having a flow channel, including: an incubating step of incubating a mixture of the antimicrobial drug and a bacterial suspension to be inspected in the flow channel of the micro-device; and a detecting step of detecting bacteria or fungi derived from the bacterial suspension to be inspected in an observation area of the flow channel of the micro-device. The detecting step can be performed by detecting an increase or decrease in the number of or a change in shape of bacteria or fungi derived from the bacterial suspension to be inspected in the observation area by a microscope or the like.Type: ApplicationFiled: August 31, 2012Publication date: November 27, 2014Applicants: OSAKA UNIVERSITY, FLUIDWARE TECHNOLOGIES INC., FUKOKU CO., LTD.Inventors: Yoshimi Matsumoto, Kohei Hayama, Shouichi Sakakihara, Kunihiko Nishino, Akihito Yamaguchi, Hiroyuki Noji, Ryota Iino
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Publication number: 20130345088Abstract: This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41?) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41?) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).Type: ApplicationFiled: March 7, 2012Publication date: December 26, 2013Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Hiroyuki Noji, Ryota Iino, Suguru Araki
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Patent number: 8524447Abstract: The object of the present invention is to provide a substance, which is easy to handle and enables the measurement of ATP with a high sensitivity regardless of the concentration of protein, and further a measuring method of ATP using the substance. Such object is solved with a fluorescence labelled fusion protein obtained by attaching two types of fluorescent substances of potential donor and acceptor for fluorescence resonance energy transfer (FRET) respectively to a protein which can cause structural changes depending on ATP binding, namely ? protein, which is the subunit of ATP synthetase, and further solved by contacting the fluorescence labelled fusion protein with a subject substance and then measuring the fluorescence spectra.Type: GrantFiled: March 25, 2008Date of Patent: September 3, 2013Assignee: The New Industry Research OrganizationInventors: Hiroyuki Noji, Hiromi Imamura, Ryota Iino, Yasuyuki Yamada
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Publication number: 20100233692Abstract: The object of the present invention is to provide a substance, which is easy to handle and enables the measurement of ATP with a high sensitivity regardless of the concentration of protein, and further a measuring method of ATP using the substance. Such object is solved with a fluorescence labelled fusion protein obtained by attaching two types of fluorescent substances of potential donor and acceptor for fluorescence resonance energy transfer (FRET) respectively to a protein which can cause structural changes depending on ATP binding, namely ? protein, which is the subunit of ATP synthetase, and further solved by contacting the fluorescence labelled fusion protein with a subject substance and then measuring the fluorescence spectra.Type: ApplicationFiled: March 25, 2008Publication date: September 16, 2010Applicants: THE NEW INDUSTRY RESEARCH ORGANIZATION, OSAKA UNIVERSITY, RIKKYO GAKUINInventors: Hiroyuki Noji, Hiromi Imamura, Ryota Iino, Yasuyuki Yamada
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Patent number: 7486440Abstract: An illumination system uses rotatable, polarized illumination optics to detect the direction of highly efficient excitation of fluorescent dyes coupled to a sample, or the absorption transition moment, using information on the direction of maximal fluorescence intensity, in which it is possible to detect individual dynamic changes in the internal structure or orientation of an entire protein molecule by coupling a single fluorescent dye molecule to the protein molecule. The polarized total internal reflection illumination optical system by rotary annulus light is also an illumination optical system in which laser beams are introduced into the objective lens of a microscope at the peripheral region by means of the rotatable illumination direction of the laser beams, and is designed to illuminate by the evanescent field that contains only transverse components that are perpendicular to the direction of radiation from the optical axis of the objective lens.Type: GrantFiled: September 18, 2003Date of Patent: February 3, 2009Assignee: Japan Science and Technology AgencyInventors: Masasuke Yoshida, Toshiharu Suzuki, Megumi Shio, Ryota Iino
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Publication number: 20080062513Abstract: The present invention relates to an illumination system that use rotatable, polarized illumination optics to detect the direction of highly efficient excitation of fluorescent dyes coupled to a sample, or the absorption transition moment, using information on the direction of maximal fluorescence intensity. The present invention also makes it possible to detect individual dynamic changes in the internal structure or orientation of an entire protein molecule by coupling a single fluorescent dye molecule to the protein molecule.Type: ApplicationFiled: September 18, 2003Publication date: March 13, 2008Inventors: Masasuke Yoshida, Toshiharu Suzuki, Megumi Shio, Ryota Iino