Patents by Inventor Ryuhei Hayashi
Ryuhei Hayashi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11649433Abstract: The present invention relates to a method for controlling differentiation of pluripotent stem cells, which method comprises selecting a laminin or a fragment thereof based on binding affinity for the pluripotent stem cells and inducing differentiation of the pluripotent stem cells in the presence of the laminin or a fragment thereof. Here, the binding affinity for cells can be assessed by time-course observation of the survival rate and motility of the cells. According to the present invention, a cell population containing any desired proportion of differentiated cells can be produced from pluripotent stem cells in a simple manner. The cell population obtained by this production method is very useful for cell therapy-based treatment strategies for diseases.Type: GrantFiled: January 31, 2018Date of Patent: May 16, 2023Assignee: Osaka UniversityInventors: Kohji Nishida, Kiyotoshi Sekiguchi, Ryuhei Hayashi, Shun Shibata
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Publication number: 20210371818Abstract: The present invention relates to a method for inducing the differentiation of corneal epithelial cells from pluripotent stem cells. More specifically, the present invention relates to a method for autonomously differentiating pluripotent stem cells, such as human iPS cells, into ectodermal cell lineage in a serum-free medium without using feeder cells and inducing the differentiation of the resultant ocular surface ectodermal lineage cells into corneal epithelial cells.Type: ApplicationFiled: June 15, 2021Publication date: December 2, 2021Applicant: Osaka UniversityInventors: Kohji NISHIDA, Ryuhei HAYASHI, Yuki ISHIKAWA
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Publication number: 20210332324Abstract: The present invention provides a method for producing a corneal epithelial cell population for the fabrication of corneal epithelial cell sheets for transplantation, the method comprising the steps of: (1) preparing a cell population containing corneal epithelial cells; (2) subjecting the cell population prepared in step (1) to magnetic-activated cell sorting and collecting CD200-negative/SSEA-4-positive cells; and (3) bringing the cells collected in step (2) into contact with a laminin selected from the group consisting of a laminin having an ?3 chain, a laminin having an ?4 chain and a laminin having an ?5 chain, or an E8 fragment thereof, and collecting cells adherent thereto.Type: ApplicationFiled: December 27, 2017Publication date: October 28, 2021Inventors: Kohji Nishida, Ryuhei Hayashi, Shun Shibata
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Patent number: 11066641Abstract: The present invention relates to a method for inducing the differentiation of corneal epithelial cells from pluripotent stem cells. More specifically, the present invention relates to a method for autonomously differentiating pluripotent stem cells, such as human iPS cells, into ectodermal cell lineage in a serum-free medium without using feeder cells and inducing the differentiation of the resultant ocular surface ectodermal lineage cells into corneal epithelial cells.Type: GrantFiled: January 13, 2016Date of Patent: July 20, 2021Assignee: Osaka UniversityInventors: Kohji Nishida, Ryuhei Hayashi, Yuki Ishikawa
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Publication number: 20210207087Abstract: Provided is a method for inducing differentiation into conjunctival epithelial cells, conjunctival goblet cells, and conjunctival epithelial stem and progenitor cells, the method comprising culturing colonies of pluripotent stem cells in a medium containing an epidermal growth factor (EGF) signaling activator to induce differentiation of the pluripotent stem cells. The present invention enables differentiation of pluripotent stem cells including iPS cells into conjunctival epithelial cells, conjunctival goblet cells, and conjunctival epithelial stem and progenitor cells and therefore is very useful for, for example, basic research on conjunctival epithelial cells and conjunctival goblet cells, regenerative therapy for intractable ocular surface diseases, and research related to the diseases.Type: ApplicationFiled: May 22, 2019Publication date: July 8, 2021Inventors: Kohji Nishida, Ryuhei Hayashi, Kimihito Nomi
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Publication number: 20210046122Abstract: The present invention addresses the problem of providing a novel therapeutic agent that is effective against diseases involving stratified squamous epithelial cells such as dry eye syndrome. The present invention is an agent for promoting normal differentiation/maturation of stratified squamous epithelial cells, which comprises a secretion of mesenchymal stem cells. It is preferable that the secretions not include animal serum, that the mesenchymal stem cells be adipose-derived mesenchymal stem cells, umbilical cord-derived mesenchymal stem cells, or bone marrow-derived mesenchymal stem cells, and that the stratified squamous epithelial cells be at least one selected from the group consisting of corneal epithelial cells, conjunctival epithelial cells, epidermal keratinocytes, oral epithelial cells, epiglottic epithelial cells, esophageal epithelial cells, vaginal epithelial cells, vocal fold epithelial cells, nasal epithelial cells, and nasal vestibular epithelial cells.Type: ApplicationFiled: January 11, 2019Publication date: February 18, 2021Applicants: OSAKA UNIVERSITY, ROHTO PHARMACEUTICAL CO., LTD.Inventors: Kohji NISHIDA, Ryuhei HAYASHI, Shun SHIBATA, Toru OKUBO, Yoichi HONMA
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Publication number: 20210024896Abstract: Provided is a method for producing a stem cell-derived lacrimal gland tissue, the method comprising isolating SSEA4 and CD104 double positive cells from a self-formed ectodermal autonomous multi-zone (SEAM) cell population derived from pluripotent stem cells and three-dimensionally culturing the isolated cells in a medium with epidermal growth factor (EGF) and a ROCK inhibitor to produce a cell population expressing a lacrimal gland-related protein. The present invention provides a lacrimal gland organoid produced from pluripotent stem cells including iPS cells and thus is very useful for cell-based regenerative therapy for lacrimal gland-related diseases and cell-based research on the diseases.Type: ApplicationFiled: March 28, 2019Publication date: January 28, 2021Inventors: Kohji Nishida, Ryuhei Hayashi, Toru Okubo, Yoichi Honma
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Publication number: 20200010800Abstract: The present invention relates to a method for controlling differentiation of pluripotent stem cells, which method comprises selecting a laminin or a fragment thereof based on binding affinity for the pluripotent stem cells and inducing differentiation of the pluripotent stem cells in the presence of the laminin or a fragment thereof. Here, the binding affinity for cells can be assessed by time-course observation of the survival rate and motility of the cells. According to the present invention, a cell population containing any desired proportion of differentiated cells can be produced from pluripotent stem cells in a simple manner. The cell population obtained by this production method is very useful for cell therapy-based treatment strategies for diseases.Type: ApplicationFiled: January 31, 2018Publication date: January 9, 2020Inventors: Kohji Nishida, Kiyotoshi Sekiguchi, Ryuhei Hayashi, Shun Shibata
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Publication number: 20190390274Abstract: An object of the present invention is to provide a method for determining anatomical sites of a body surface tissue. Provided is a method for determining an anatomical site of origin of a body surface tissue specimen, the method comprising the steps of: (A) selecting at least 8 kinds of genes from the group consisting of a Hox gene family and a Pax6 gene; (B) measuring the expression levels of the genes selected in the above (A) in the body surface tissue specimen; and (C) determining the anatomical site of origin of the body surface tissue specimen based on the expression levels or a combination of the expression levels measured in the above (B).Type: ApplicationFiled: January 26, 2018Publication date: December 26, 2019Inventors: Kohji Nishida, Ryuhei Hayashi, Toru Okubo
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Patent number: 10457913Abstract: The present invention relates to a method for inducing a keratin 12-positive corneal epithelioid cell from a surface ectoderm-derived cell. More specifically, the present invention relates to a method for inducing a keratin 12-positive corneal epithelioid cell, comprising introducing PAX6, KLF4, and OCT4 into a surface ectoderm-derived cell, such as an oral mucosal epithelial cell, and a corneal epithelioid cell induced by the method.Type: GrantFiled: January 12, 2016Date of Patent: October 29, 2019Assignee: Osaka UniversityInventors: Kohji Nishida, Yuzuru Sasamoto, Ryuhei Hayashi
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Patent number: 10352869Abstract: An inspection apparatus includes an illumination device including an arch-like lighting unit that is provided around an inspection target in a circular arc form and emits light toward the inspection target, imaging devices that capture images of a light reflection surface of the inspection target by which the light emitted from the arch-like lighting unit is reflected, and a determination device that inspects the light reflection surface of the inspection target on the basis of the images captured by the imaging devices. As a result, the inspection apparatus provides an effect of preventing the apparatus from being increased in size.Type: GrantFiled: October 5, 2017Date of Patent: July 16, 2019Assignee: YAZAKI CORPORATIONInventor: Ryuhei Hayashi
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Publication number: 20180160495Abstract: A lighting device includes: a variable voltage source; a transistor which controls a current flowing through a light-emitting element; and a control circuit which causes the transistor to pass a current corresponding to a received instruction for a dimming level through the light-emitting element. When the received instruction instructs at least a predetermined dimming Level, the control circuit causes a voltage drop in the transistor to become a first voltage by controlling the variable voltage source, and when the received instruction instructs a dimming level less than the predetermined dimming level, the control circuit causes the voltage drop to become a second voltage higher than the first voltage, by controlling the variable voltage source.Type: ApplicationFiled: December 2, 2017Publication date: June 7, 2018Inventors: Shinichi MURAKAMI, Kazuhiro Kumada, Ryuhei Hayashi
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Patent number: 9992837Abstract: A lighting device includes: a variable voltage source; a transistor which controls a current flowing through a light-emitting element; and a control circuit which causes the transistor to pass a current corresponding to a received instruction for a dimming level through the light-emitting element. When the received instruction instructs at least a predetermined dimming level, the control circuit causes a voltage drop in the transistor to become a first voltage by controlling the variable voltage source, and when the received instruction instructs a dimming level less than the predetermined dimming level, the control circuit causes the voltage drop to become a second voltage higher than the first voltage, by controlling the variable voltage source.Type: GrantFiled: December 2, 2017Date of Patent: June 5, 2018Assignee: Panasonic Intellectual Property Management, Co., Ltd.Inventors: Shinichi Murakami, Kazuhiro Kumada, Ryuhei Hayashi
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Publication number: 20180148685Abstract: The present invention relates to a method for inducing a keratin 12-positive corneal epithelioid cell from a surface ectoderm-derived cell. More specifically, the present invention relates to a method for inducing a keratin 12-positive corneal epithelioid cell, comprising introducing PAX6, KLF4, and OCT4 into a surface ectoderm-derived cell, such as an oral mucosal epithelial cell, and a corneal epithelioid cell induced by the method.Type: ApplicationFiled: January 12, 2016Publication date: May 31, 2018Applicant: Osaka UniversityInventors: Kohji Nishida, Yuzuru SASAMOTO, Ryuhei HAYASHI
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Publication number: 20180106728Abstract: An inspection apparatus includes an illumination device including an arch-like lighting unit that is provided around an inspection target in a circular arc form and emits light toward the inspection target, imaging devices that capture images of a light reflection surface of the inspection target by which the light emitted from the arch-like lighting unit is reflected, and a determination device that inspects the light reflection surface of the inspection target on the basis of the images captured by the imaging devices. As a result, the inspection apparatus provides an effect of preventing the apparatus from being increased in size.Type: ApplicationFiled: October 5, 2017Publication date: April 19, 2018Applicant: Yazaki CorporationInventor: Ryuhei HAYASHI
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Publication number: 20180010093Abstract: The present invention relates to a method for inducing the differentiation of corneal epithelial cells from pluripotent stem cells. More specifically, the present invention relates to a method for autonomously differentiating pluripotent stem cells, such as human iPS cells, into ectodermal cell lineage in a serum-free medium without using feeder cells and inducing the differentiation of the resultant ocular surface ectodermal lineage cells into corneal epithelial cells.Type: ApplicationFiled: January 13, 2016Publication date: January 11, 2018Applicant: Osaka UniversityInventors: Kohji Nishida, Ryuhei HAYASHI, Yuki ISHIKAWA
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Patent number: 9795012Abstract: When a controller receives an external instruction for initial setting, the controller gives image sensors an instruction regarding lighting. When an image sensor among the image sensors receives the instruction regarding lighting from the controller, the image sensor performs lighting control in accordance with the instruction regarding lighting on a luminaire among luminaires to determine (i) a relative position of the luminaire relative to the image sensor and (ii) a relative position of the image sensor relative to a different image sensor among the image sensors. The controller determines positions of the image sensors and positions of the luminaires by collecting the relative positions of the image sensors and the relative positions of the luminaires determined by the image sensors.Type: GrantFiled: October 27, 2016Date of Patent: October 17, 2017Assignee: PANASONIC INTELLECTUAL PROPERTY MANAGEMENT CO., LTD.Inventors: Ryuhei Hayashi, Yuichi Ejiri
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Publication number: 20170127498Abstract: When a controller receives an external instruction for initial setting, the controller gives image sensors an instruction regarding lighting. When an image sensor among the image sensors receives the instruction regarding lighting from the controller, the image sensor performs lighting control in accordance with the instruction regarding lighting on a luminaire among luminaires to determine (i) a relative position of the luminaire relative to the image sensor and (ii) a relative position of the image sensor relative to a different image sensor among the image sensors. The controller determines positions of the image sensors and positions of the luminaires by collecting the relative positions of the image sensors and the relative positions of the luminaires determined by the image sensors.Type: ApplicationFiled: October 27, 2016Publication date: May 4, 2017Inventors: Ryuhei HAYASHI, Yuichi EJIRI
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Patent number: 9347041Abstract: The present invention relates to a method for preparing corneal endothelial progenitor cells by adherent culturing a cell population isolated from corneal endothelial cell tissue at a low density by using a serum-free medium. The present invention also relates to a method for preparing corneal endothelial cells by differentiation-inducing the corneal endothelial progenitor cells obtained by the aforementioned method. According to the present invention, corneal endothelial progenitor cells can be selectively grown from a corneal tissue-derived cell population, and corneal endothelial cells obtained by inducing the corneal endothelial progenitor cells can be applied to treatment of corneal endothelial diseases. As a result, problems of corneal transplantation such as shortage of donors and occurrence of rejection can be solved.Type: GrantFiled: July 13, 2012Date of Patent: May 24, 2016Assignee: OSAKA UNIVERSITYInventors: Ryuhei Hayashi, Susumu Hara, Tomofumi Kageyama, Kohji Nishida
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Publication number: 20150351380Abstract: The present invention provides a tissue preservation solution that has good quality for preservation of tissues, organs and cells, and is useful in the fields of regenerative medicine, transplantation medicine, etc. The tissue preservation solution of the present invention comprises a compound having a Nrf2 activating effect. Preferable compounds having a Nrf2 activating effect are electrophiles, and in particular, ebselen and tert-butylhydroquinone. A preferable basal solution of the tissue preservation solution is Hank's balanced salt solution.Type: ApplicationFiled: July 3, 2013Publication date: December 10, 2015Inventors: Ryuhei Hayashi, Kohji Nishida, Ryosuke Katori