Abstract: The one aspect of the present invention aims to provide a novel cloning method for human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), which method is based on culture of dispersed single cells without addition of an apoptotic agent. More specifically, the one aspect of the present invention aims to provide a cloning method for hESCs and hiPSCs based on culture of dispersed single cells utilizing shear stress. The above problem is solved by providing a method for culturing dispersed single pluripotent cells, wherein the dispersed single cells are cultured under laminar flow conditions.

Abstract: A protein detection device and method. The device has a main channel in which is attached either a polar rail molecule or a biomolecular motor which moves on the rail molecule in a direction corresponding to the polarity of the rail molecule. A sub-channel crosses the main channel for receiving a sample including tau-protein. The method includes feeding the sample to the device and detecting a ratio of mutated tau-protein included in the sample, based on motion of the biomolecular motor on the rail molecule or on motion of the rail molecule moved by action of the biomolecular motor.

Abstract: An object is to move a rail molecule by means of a biomolecular motor deposited on a base and inactivate the biomolecular motor through irradiation with light having a predetermined wavelength, to thereby readily and reliably fix the rail molecule at a predetermined position, while orienting the rail molecule in a predetermined direction without employment of any reagent. A method for fixing a rail molecule which has polarity and on which a biomolecular motor moves in a direction corresponding to the polarity includes depositing a biomolecular motor on a base; moving a rail molecule by means of the biomolecular motor; and inactivating the biomolecular motor by irradiating the biomolecular motor with light having a predetermined wavelength when the rail molecule reaches a predetermined position, to thereby fix the rail molecule so that it is oriented in a predetermined direction.

Abstract: An object is to move a rail molecule by means of a biomolecular motor deposited on a base and inactivate the biomolecular motor through irradiation with light having a predetermined wavelength, to thereby readily and reliably fix the rail molecule at a predetermined position, while orienting the rail molecule in a predetermined direction without employment of any reagent. A method for fixing a rail molecule which has polarity and on which a biomolecular motor moves in a direction corresponding to the polarity includes depositing a biomolecular motor on a base; moving a rail molecule by means of the biomolecular motor; and inactivating the biomolecular motor by irradiating the biomolecular motor with light having a predetermined wavelength when the rail molecule reaches a predetermined position, to thereby fix the rail molecule so that it is oriented in a predetermined direction.

Abstract: An object is to move a rail molecule by means of a biomolecular motor deposited on a base and inactivate the biomolecular motor through irradiation with light having a predetermined wavelength, to thereby readily and reliably fix the rail molecule at a predetermined position, while orienting the rail molecule in a predetermined direction without employment of any reagent. A method for fixing a rail molecule which has polarity and on which a biomolecular motor moves in a direction corresponding to the polarity includes depositing a biomolecular motor on a base; moving a rail molecule by means of the biomolecular motor; and inactivating the biomolecular motor by irradiating the biomolecular motor with light having a predetermined wavelength when the rail molecule reaches a predetermined position, to thereby fix the rail molecule so that it is oriented in a predetermined direction.