Abstract: Apolipoprotein A-1 Milano (ApoA-1M), the protein component of a high-density lipoprotein (HDL) mimic with promising potential for reduction of atherosclerotic plaque, is produced on a large scale by expression in E. coli. Significant difficulty with clearance of host cell proteins (HCPs) was experienced in the original manufacturing process, despite lengthy purification. Analysis of purified protein solutions and intermediate process samples led to the identification of several major HCPs, and a bacterial protease causing the production of a truncated species of ApoA-1M co-purifying with the product. Deletions in these genes from the original host strain succeeded in substantially reducing the levels of HCPs without adversely affecting overall fermentation productivity, contributing to a much more efficient and robust new manufacturing process.

Abstract: A process using a hydrophobic membrane separation process for making an enhanced flavor fluid, particularly a low-alcohol brew, having improved flavor is disclosed.

Abstract: A process using a hydrophobic membrane separation process for making an enhanced flavor fluid, particularly a low-alcohol brew, having improved flavor is disclosed.

Abstract: A process using a hydrophobic membrane separation process for making an enhanced flavor fluid, particularly a low-alcohol brew, having improved flavor is disclosed.

Abstract: The present invention is directed to a solid poly-amphiphilic polymer. The polymer may be (1) a continuous film (a) which is strengthened sufficiently by cross-linking to be used alone and/or supported on a frame, (b) overlaid and/or cast on a porous hydrophobic support or (2) introduced into the pores of a microporous hydrophobic membrane. The present invention is also a process for selectively removing a dissolved species (solute or target compound) from an aqueous solution or from a gaseous stream comprising contacting said aqueous solution or gaseous stream having the dissolved species and an aqueous stripping solution or other means for removing said species with opposite sides or surfaces.

Abstract: A process for removing dissolved organic compounds from an aqueous solution wherein said solution is contacted with a water insoluble, liquid oligomeric or polymeric poly(amphiphilic) compound having an affinity for said organic compound.

Abstract: An immobilized liquid membrane and a method for selective extraction using same wherein said membrane comprises a hydrophobic polyamphiphilic oligomeric or polymeric liquid supported or immobilized within a microporous support which may be hydrophobic.

Abstract: A process for the in-situ remediation of a contaminated heterogeneous soil region comprising: (a) introducing material for treating contaminants in a contaminated heterogeneous soil region into at least one liquid permeable region within the contaminated heterogeneous soil region to form at least one treating zone within the contaminated heterogeneous soil region, (b) transmitting direct electric current through at least one low permeability soil region within the contaminated heterogeneous soil region between a first electrode and a second electrode having opposite charge, wherein (i) the first electrode is located at a first end of the contaminated heterogeneous soil region and the second electrode is located at the opposite end of the contaminated heterogeneous soil region or (ii) the first electrode is located at a first end of each of the low permeability soil regions and the second electrode is located at the opposite end of each of the low permeability soil regions, (1) to cause an electroosmotic flow

Abstract: A process for the in-situ remediation of contaminated soil comprising: (a) forming at least one liquid permeable region within a contaminated soil region, (b) introducing material for treating contaminants in the contaminated soil region into the liquid permeable regions to form at least one treating zone within the contaminated soil region, and (c) transmitting direct electric current through the contaminated soil region between a first electrode and a second electrode having opposite charge, wherein the first electrode is located at a first end of the contaminated soil region and the second electrode is located at the opposite end of the contaminated soil region to cause an electroosmotic flow from the second to the first electrode.

Abstract: A method for the solubilization and naturation of somatotropin from refractile bodies produced by r-DNA technology wherein the refractile bodies are dissolved in an aqueous solution comprising a chaotropic agent such as urea or guanidine hydrochloride and a soluble organic alcohol such as isopropanol or benzyl alcohol. The solubilized protein is exposed to mild oxidation for a time sufficient to allow the protein to form disulfide bonds and refold to its native conformation. The presence of the alcohol suppresses the formation of somatotropin dimers and aggregates and results in higher yields of the desirable monomeric form of the protein.

Abstract: A method for the solubilization and naturation of somatotropin from refractile bodies produced by r-DNA technology wherein the refractile bodies are dissolved in an aqueous solution comprising a chaotropic agent such as urea or guanidine hydrochloride and a soluble surfactant such as sodium dodecylsulfate. The solubilized protein is exposed to mild oxidation for a time sufficient to allow the protein to form disulfide bonds and refold to its native conformation. The presence of the surfactant suppresses the formation of somatotropin dimers and aggregates and results in higher yields of the desirable monomeric form of the protein.

Abstract: An improved process for the preparation of 4-methyl thiazole is disclosed. The process utilizes a substituted imine and sulfur dioxide heated in the presence of a catalyst. The thiazoles are known important chemical intermediates.