Abstract: Embodiments of fiber substrate-based fluidic device and methods of manufacturing the same are described herein. In one example, a microfluidic diagnostic device for detecting a presence and/or concentration of an analyte includes a nitrocellulose substrate sandwiched between two bonded layers of a hydrophobic film. The nitrocellulose substrate includes a plurality of wells and/or fluidic channels formed in the nitrocellulose substrate such that boundaries of the wells and/or fluidic channels are defined by the nitrocellulose and at least one of the bonded layers of the hydrophobic film.

Abstract: Embodiments of fiber substrate-based fluidic device and methods of manufacturing the same are described herein. In one example, a method of manufacturing a fiber substrate-based fluidic device comprises bonding a hydrophobic film that is substantially transparent to a laser beam (e.g., a film that transmits at least 80% of the incident radiant power of the laser beam) to a hydrophilic substrate and ablating the hydrophilic substrate with the laser beam without ablating the hydrophobic film to form one or more fluidly sealed channels in the hydrophilic substrate.

Abstract: Embodiments of fiber substrate-based fluidic device and methods of manufacturing the same are described herein. In one example, a method of manufacturing a fiber substrate-based fluidic device comprises bonding a hydrophobic film that is substantially transparent to a laser beam (e.g., a film that transmits at least 80% of the incident radiant power of the laser beam) to a hydrophilic substrate and ablating the hydrophilic substrate with the laser beam without ablating the hydrophobic film to form one or more fluidly sealed channels in the hydrophilic substrate.