Patents by Inventor Sam Woo
Sam Woo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8361807Abstract: Various embodiments described in the application relate to an apparatus, system, and method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise an apparatus comprising at least one conduit operatively connected to one or more pumps for providing discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.Type: GrantFiled: March 22, 2011Date of Patent: January 29, 2013Assignee: Applied Biosystems, LLCInventors: Willy Wiyatno, Linda Lee, David Cox, Richard Reel, Mark Oldham, Jim Nurse, Douglas Greiner, Dennis Lehto, Janusz Wojtowicz, Sam Woo
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Publication number: 20120270211Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.Type: ApplicationFiled: February 15, 2012Publication date: October 25, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Linda LEE, Sam WOO, Peter MA
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Publication number: 20110124111Abstract: Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 ?l). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.Type: ApplicationFiled: August 31, 2010Publication date: May 26, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Jon HOSHIZAKI, Joon Mo YANG, Maryam SHARIATI, David COX, Kirk HIRANO, John BRIDGHAM, Sam WOO, George GOLDA
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Publication number: 20110052446Abstract: Various flowcell configurations and systems are provided as are methods of making and using same. The flowcells, systems, and methods of use can be useful in carrying out sequencing reactions and next generation sequencing methods.Type: ApplicationFiled: August 31, 2010Publication date: March 3, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Kirk HIRANO, Mark ANDERSEN, Jian GONG, Sam WOO, David COX, Joon Mo YANG, Min YUE, Maryam SHARIATI, John BRIDGHAM, David LIU
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Publication number: 20100261230Abstract: A system and method are provided for large volume sample amplification adaptable for use with conventional PCR-based reactions as well as emulsion-based PCR reactions. A sample is retained in a pouch or flexible bag which permits bulk PCR amplification with efficient heat-transfer properties. For applications involving emulsion-based PCR amplification, the system and method provide improved uniformity in emulsion amplification and can be used to amplify large or small volume emulsions rapidly and reproducibly.Type: ApplicationFiled: April 8, 2010Publication date: October 14, 2010Applicant: APPLIED BIOSYSTEMS, LLCInventors: David LIU, Joon Mo YANG, Maryam SHARIATI, Sam WOO, David M. COX, Dennis A. LEHTO
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Publication number: 20070238186Abstract: System for detection and/or analysis of nucleic acids using nanowires to detect covalent modification of nucleic acids.Type: ApplicationFiled: March 29, 2006Publication date: October 11, 2007Inventors: Hongye Sun, Steven Fung, Sam Woo
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Publication number: 20070141593Abstract: Various embodiments of the teachings relate to a system or method for sample preparation or analysis in biochemical or molecular biology procedures. The sample preparation can involve small volume processed in discrete portions or segments or slugs, herein referred to as discrete volumes. A molecular biology procedure can be nucleic acid analysis. Nucleic acid analysis can be an integrated DNA amplification/DNA sequencing procedure.Type: ApplicationFiled: August 22, 2006Publication date: June 21, 2007Inventors: Linda Lee, Sam Woo, Congcong Ma, Richard Reel, Mark Oldham, David Cox, Benjamin Schroeder, Jon Sorenson, Willy Wiyatno
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Publication number: 20070068573Abstract: Various embodiments described in the application relate to an apparatus, system, and method for fluidically controlling, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise combinations of detectors, controllers, valves, and fluid supply units to control the spatial size, location and/or fluidic composition of discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.Type: ApplicationFiled: August 22, 2006Publication date: March 29, 2007Applicant: Applera CorporationInventors: David Cox, Sam Woo, Steven Boege, Mark Oldham, Richard Reel, Eric Nordman, Linda Lee, Willy Wiyatno, Dmitry Sagatelyan
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Publication number: 20070062583Abstract: Various embodiments described in the application relate to an apparatus, system, and method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise an apparatus comprising at least one conduit operatively connected to one or more pumps for providing discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.Type: ApplicationFiled: August 22, 2006Publication date: March 22, 2007Inventors: David Cox, Willy Wiyatno, Mark Oldham, James Nurse, Douglas Greiner, Sam Woo, Richard Reel, Dennis Lehto, Linda Lee, Janusz Wojtowicz
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Publication number: 20070026434Abstract: The present invention relates generally to nucleobase polymer functionalizing reagents, to mobility-modified sequence-specific nucleobase polymers, to compositions comprising a plurality of mobility-modified sequence-specific nucleobase polymers, and to the use of such polymers and compositions in a variety of assays, such as, for example, for the detection of a plurality of selected nucleotide sequences within one or more target nucleic acids. The mobility-modifying polymers of the present invention include phosphoramidite reagents which can be joined to other mobility-modifying monomers and to sequence-specific oligonucleobase polymers via uncharged phosphate triester linkages. Addition of the mobility-modifying phosphoramidite reagents of the present invention to oligonucleobase polymers results in unexpectedly large effects the mobility of those modified oligonucleobase polymers, especially upon capillary electrophoresis in non-sieving media.Type: ApplicationFiled: July 11, 2006Publication date: February 1, 2007Applicant: Applera CorporationInventors: Sam Woo, Ron Graham, Jing Tian
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Publication number: 20060288758Abstract: Disclosed herein is a calibration apparatus for vacuum gauge having a combined use for an 1 Torr level, a 10 Torr level, and a 100 Torr level vacuum gauge.Type: ApplicationFiled: December 14, 2005Publication date: December 28, 2006Applicant: Korea Research Institute of Standards and ScienceInventors: Sam Woo, In Choi, Boo Kim
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Publication number: 20060063734Abstract: Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2?,4?,5?,7?-tetrachloro-4,7-dichloro-5- (and 6-)carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.Type: ApplicationFiled: November 7, 2005Publication date: March 23, 2006Applicant: Applera CorporationInventors: Steven Menchen, Linda Lee, Charles Connell, N. Hershey, Vergine Chakerian, Sam Woo, Steven Fung
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Publication number: 20050112591Abstract: The present teachings relate to methods of purifying, isolating, separating, and identifying target nucleic acids. In some embodiments of the present teachings, an affinity moiety can be incorporated into one of the flanking primers of a nucleic acid amplification reaction primer pair. The reaction mixture can be contacted with a binding moiety specific for the affinity moiety, thereby allowing immobilization of the double stranded amplification product, separation of reaction components lacking the affinity moiety, and isolation of the target nucleic acid strand. Further, one of the flanking primers of the nucleic acid amplification reaction can comprise a label and a mobility modifier, thereby facilitating identification of the target nucleic acids. In some embodiments, the amplification reaction is multiplexed and comprises polymorphic microsatellites useful in human identification. and the manufacturing of molecular size standards.Type: ApplicationFiled: November 25, 2003Publication date: May 26, 2005Applicant: Applera CorporationInventors: Pero Dimsoski, Sam Woo
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Publication number: 20050112609Abstract: Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.Type: ApplicationFiled: April 14, 2004Publication date: May 26, 2005Inventors: Paul Grossman, Steven Fung, Steven Menchen, Sam Woo, Emily Winn-Deen
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Publication number: 20050112608Abstract: Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In one embodiment of the invention, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe pair includes two polynucleotide probe elements which are complementary in sequence to adjacent portions of a selected one of the target sequences in the target polynucleotide. In each probe pair, one of the probe elements contains a non-polynucleotide polymer chain which imparts a distinctive mobility to the associated probe pair, when the elements in the pair are ligated. The other element in the pair contains a detectable reporter label. After the probe pairs have been allowed to hybridize with the target polynucleotide, the hybridized polynucleotides are treated under conditions effective to ligate the end subunits of target-bound probe elements when their end subunits are base-paired with adjacent target bases.Type: ApplicationFiled: April 14, 2004Publication date: May 26, 2005Inventors: Paul Grossman, Steven Fung, Steven Menchen, Sam Woo, Emily Winn-Deen
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Publication number: 20050084870Abstract: Long wavelength, narrow emission bandwidth fluorescein dyes are provided for detecting specially overlapping target substances. The dyes comprise 4,7-dichlorofluorescein, and particularly 2?, 4?,5?,7?-tetrachloro-4,7-dichloro-5-(and 5-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.Type: ApplicationFiled: November 14, 2003Publication date: April 21, 2005Applicant: Applera CorporationInventors: Steven Menchen, Linda Lee, Charles Connell, N. Hershey, Vergine Chakerian, Sam Woo, Steven Fung
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Publication number: 20050042644Abstract: The present invention relates generally to nucleobase polymer functionalizing reagents, to mobility-modified sequence-specific nucleobase polymers, to compositions comprising a plurality of mobility-modified sequence-specific nucleobase polymers, and to the use of such polymers and compositions in a variety of assays, such as, for example, for the detection of a plurality of selected nucleotide sequences within one or more target nucleic acids. The mobility-modifying polymers of the present invention include phosphoramidite reagents which can be joined to other mobility-modifying monomers and to sequence-specific oligonucleobase polymers via uncharged phosphate triester linkages. Addition of the mobility-modifying phosphoramidite reagents of the present invention to oligonucleobase polymers results in unexpectedly large effects the mobility of those modified oligonucleobase polymers, especially upon capillary electrophoresis in non-sieving media.Type: ApplicationFiled: May 28, 2004Publication date: February 24, 2005Applicant: Applera CorporationInventors: Sam Woo, Ron Graham, Jing Tian
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Patent number: 5400150Abstract: A screen encore method capable of displaying again (encore) a block of pictures designated by a user or a last picture with a single key by software control, without adding hardware, so that the inventive system is highly improved both in its function and quality.Type: GrantFiled: October 6, 1989Date of Patent: March 21, 1995Assignee: SamSung Electronics Co., Ltd.Inventor: Jong-Sam Woo
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Patent number: 5287232Abstract: An automatic (auto) tape searching method which searches the required tape portion fast and automatically by repeating a sequence of operational procedures. The auto tape searching method comprises: a first stage which initializes the port of a microcomputer and the content of a RAM (random access memory), sets the initial values, and controls interrupts at a power-on reset of a video tape recording system; a second stage which checks key input and, for auto random search (ARS) key, performs the auto random search function; a third stage which performs mode checking and, if a present mode needs to accompany the mechanism operation, controls the present mode; and a fourth stage which switches the mode and checks the sensor and an emergency state to make the system in stable condition.Type: GrantFiled: September 25, 1989Date of Patent: February 15, 1994Assignee: Samsung Electronics Co., Ltd.Inventor: Jong-Sam Woo
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Patent number: 5210661Abstract: There is disclosed a method of skipping unrecorded area during playback in order to minimize an interruption time upon playing back a recorded program in a tape drive. The method is performed while playing back a record medium, such that an area with no videos and audios is played back at a high speed and an area with videos and audios is played back at a normal speed, resulting in minimized time for playing back unrecorded areas, thus yielding another advantage in that an audience is kept from being bored and, furthermore, power is saved by automatically turning the power-off when the record medium playback is completed.Type: GrantFiled: September 7, 1990Date of Patent: May 11, 1993Assignee: Samsung Electronics Co., Ltd.Inventor: Jong-Sam Woo