Abstract: Various biodegradable polyglutamate conjugates comprising recurring units of the general formulae (I), (II), (III), (IV), (V), and/or (VI) are prepared. The polymers are conjugated with a plurality of drugs. Such polymer conjugates are useful for variety of drug, targeting, stabilizing and/or imaging agent delivery applications.

Abstract: Complexes are disclosed which are a combination of a polynucleotide and a biodegradable polyacetal-peptide. The disclosed complexes are useful for cell transfection.

Abstract: Various biodegradable polyglutamate polymer conjugates that can include recurring units of the general formulae (I) and (II) are described herein. Such polymer conjugates are useful for variety of drug, targeting, stabilizing and/or imaging agent delivery applications.

Abstract: Various biodegradable polyglutamate-amino acids comprising recurring units of the general formulae (I) and (II) are prepared. Such polymers are useful for variety of drug, targeting, stabilizing and/or imaging agent delivery applications.

Abstract: A microscope system, kit and method are described for observation of cellular activity in real time. The system is used to monitor cell transfection using a polynucleotide noncovalently attached to a carrier, The carrier labeled with a dye or fluorescent agent so that transport of complex can be monitored in real time. The polynucleotide includes both a gene of interest and a reporter gene. The reporter gene indicates successful expression of the gene of interest.

Abstract: The present invention provides visible light sensitive and ultraviolet (UV) light sensitive composition for DNA transfer comprising acid sensitive polyacetals developed as DNA/RNA delivery agents, a photoacid generator and optionally a photosensitizer.

Abstract: Various biodegradable polyglutamate-amino acids comprising recurring units of the general formulae (I) and (II) are prepared. Such polymers are useful for variety of drug, biomolecule and imaging agent delivery applications.

Abstract: A synthetic polymer comprises a cationic recurring unit and a crosslinking unit, wherein the crosslinking unit comprises at least a first degradable unit (preferably acid-labile) and at least a second degradable unit (preferably hydrolyzable). A carrier composition comprising the synthetic polymer and a bioactive agent is useful for the delivery of the bioactive agent into the nuclei of the cells.

Abstract: A polymer comprising a recurring unit of the formula (I) is described. In the formula (I), X is selected from the group consisting of —(CH2CH2O)m—CH2CH2— and —CH2CH2CH2O—(CH2CH2O)m—CH2CH2CH2—, wherein m is an integer in the range of 1 to 100; Y is selected from the group consisting of —C*HCH2—, —C*HCH2CH2—, —C*H—NHC(?O)—CH2CH2—, —C*H—NHC(?O)—CH2CH2CH2—, —CH2CH2N*CH2CH2—, —CH2CH2CH2N*CH2CH2CH2—, C2 to C20 alkyl, and C6 to C20 aryl, wherein C* and N* represent atoms to which Z is bonded; and Z is selected from the group consisting of —NHR1, —NH—C(?O)—(CH2)nC(?O)NR1R1, —NH—C(?O)—(CH2)nC(?O)OR1, —(CH2)nC(?O)NR1R2, —(CH2)nC(?O)OR1, —(CH2)nC(?O)SR1, and —(CH2)nNR1R2, wherein n is an integer in the range of 1 to 3, wherein R1 and R2 are each independently selected from the group consisting of hydrogen, C1 to C20 alkyl, C6 to C20 aryl, anticancer drugs, peptides, antibody fragment, lactose, galactose, mannose, transferrin, magnetic resonance imaging agents, succinimyl, and alkali metal.

Abstract: Condensation polymerization methods are used to prepare various biodegradable polyacetals of the general formula (D). Such polymers are useful for variety of drug, biomolecule and imaging agent delivery applications.

Abstract: Polymers and methods of making them are described. The polymers may be used to coat living cells. The polymer-coated cells are useful in cell therapy applications.

Abstract: Polyacetals comprising a recurring units represented by a formula selected from the group consisting of formula (I) and (II): wherein A comprises at least one acetal group; B is selected from the group consisting of —CH—, —CH(CH3)—, CH2CH—, —CH2C(CH3)—, —CH(CH3)CH—, and —CHCH(CH3)CH(CH3)—; Z is selected from the group consisting of C(O)OR1, C(O)SR1, C(O)NR1R2, and VU; V is a linker group; U is selected from the group consisting of poly(ethyleneimine), poly(propyleneimine), poly(lysine), PAMAM dendrimer, octaamine dendrimer, hexadecaamine dendrimer, enhancer, and targeting receptor; R1 and R2 are each individually selected from the group consisting of hydrogen, C1 to C10 alkyl, and C6 to C10 aryl; D is a linkage selected from the group consisting of carboxylic amide, carboxylic ester, urea, and urethane; and G is selected from the group consisting of C4 to C20 alkyl, C6-C10 aryl, and —(OCH2CH2)n—, where n is in the range of 1 to about 250, are useful in nucleic acid delivery applications.

Abstract: Complexes are disclosed which are a combination of a polynucleotide and a biodegradable polyacetal-peptide. The disclosed complexes are useful for cell transfection.

Abstract: A microscope system, kit and method are described for observation of cellular activity in real time. The system is used to monitor cell transfection using a polynucleotide noncovalently attached to a carrier, The carrier labeled with a dye or fluorescent agent so that transport of complex can be monitored in real time. The polynucleotide includes both a gene of interest and a reporter gene. The reporter gene indicates successful expression of the gene of interest.

Abstract: A synthetic polymer comprises a cationic recurring unit and a crosslinking unit, wherein the crosslinking unit comprises at least a first degradable unit (preferably acid-labile) and at least a second degradable unit (preferably hydrolyzable). A carrier composition comprising the synthetic polymer and a bioactive agent is useful for the delivery of the bioactive agent into the nuclei of the cells.

Abstract: Degradable complexes comprising a polycation, a polyanion and a polynucleotide are useful for in vivo polynucleotide delivery applications.

Abstract: A polymer comprising recurring units represented by formula (I): wherein X is selected from the group consisting of C(O)OR1, C(O)SR1, C(O)NR1R2, and VZ, where R1 and R2 are each individually selected from the group consisting of hydrogen, C1 to C10 alkyl, and C6 to C10 aryl, where V is a labile linker group, and where Z is selected from the group consisting of poly(ethyleneimine), poly(propyleneimine), poly(lysine), PAMAM dendrimer, octaamine dendrimer, and hexadecaamine dendrimer; and wherein Y is selected from the group consisting of —(CH2)2—, —(CH2)2—O—(CH2)2—, —(CH2)2—O—(CH2)2—, and —(CH2)3—NHC(O)—(CH2)6—C(O)NH—(CH2)3— is useful in nucleic acid delivery applications. Polyacetals of the formula (I) are preferably made by reacting appropriate diols and divinyl ethers. In preferred embodiments, complexes formed between polyacetals of the formula (I) and polynucleotides are useful as transfection reagents.

Abstract: Polyacetals comprising a recurring units represented by a formula selected from the group consisting of formula (I) and (II): wherein A comprises at least one acetal group; B is selected from the group consisting of —CH—, —CH(CH3)—, CH2CH—, —CH2C(CH3)—, —CH(CH3)CH—, and —CHCH(CH3)CH(CH3)—; Z is selected from the group consisting of C(O)OR1, C(O)SR1, C(O)NR1R2, and VU; V is a linker group; U is selected from the group consisting of poly(ethyleneimine), poly(propyleneimine), poly(lysine), PAMAM dendrimer, octaamine dendrimer, hexadecaamine dendrimer, enhancer, and targeting receptor; R1 and R2 are each individually selected from the group consisting of hydrogen, C1 to C10 alkyl, and C6 to C10 aryl; D is a linkage selected from the group consisting of carboxylic amide, carboxylic ester, urea, and urethane; and G is selected from the group consisting of C4 to C20 alkyl, C6-C10 aryl, and —(OCH2CH2)n—, where n is in the range of 1 to about 250, are useful in nucleic acid delivery applications.

Abstract: The present invention provides visible light sensitive and ultraviolet (UV) light sensitive composition for DNA transfer comprising acid sensitive polyacetals developed as DNA/RNA delivery agents, a photoacid generator and optionally a photosensitizer.

Abstract: A polymer comprising recurring units represented by formula (I): 1