Abstract: A method for using Cold Atmospheric Plasma based T cell therapy for treating cancer including treating tumor cells with cold atmospheric plasma to permeabilize the tumor cells, condensing a mammalian CRISPR plasmid vector into compact nanostructures using one of poly-L-lysine (PLL) and Star-shaped poly(ethylene glycol)-block-polyethylenimine, and transfecting tumor cells with the catalytically inactive condensed CRISPR plasmid vector containing transcriptional activators (dcas9-VP64-sgRNA-CRISPRa) to elicit immune responses by enhancing the presentation of tumor-associated antigens.

Abstract: A method for performing cold atmospheric plasma treatment of respiratory infections or lung cancer having a source of a carrier gas, a cold atmospheric plasma generator connected to the source of carrier gas, a source of compressed air, a humidifier connected to the source of compressed air, a source of oxygen, a ventilator having inputs connected to an output of the humidifier and the source of oxygen, a mixer having an interior chamber formed from a dielectric, an active electrode inside the interior chamber, and an outer electrode connected to ground, wherein the mixer has a fluid input port connected to a gas output of the cold atmospheric plasma generator and an output of the ventilator, and a delivery member connected to an output of the mixer for delivering combined humidified air and cold atmospheric plasma to a respiratory system of a patient.

Abstract: A method for producing cold atmospheric plasma-produced exosomes ex vivo for use in systemic treatment of cancer. The method comprises isolating patient cancerous tumor primary cells, culturing isolated patient cancerous tumor primary cells in appropriate culture media, sequencing said isolated patient cancerous tumor primary cells and identifying mutant genes, transfecting mutant genes into pots of cells, wherein the transformed cells overexpress a unique mutant protein, treating the pots of transformed cells with cold atmospheric plasma to cause apoptosis of said transformed cells, wherein apoptotic cells secrete exosomes containing oxidized mutant proteins, isolating said exosomes containing oxidized mutant proteins, and storing said exosomes. The method may further comprise injecting said exosomes into a patient to systemically treat cancer.

Abstract: A method for producing microvesicles ex vivo for use in systemic treatment of cancer. The method comprises isolating patient cancerous tumor primary cells, culturing isolated patient cancerous tumor primary cells in appropriate culture media, treating cultured patient cancerous tumor primary cells non cold atmospheric plasma, after apoptosis of cultured patient cancerous tumor primary cells occurs, collecting apoptotic cell-derived extracellular microvesicles from the culture media by differential centrifugation, directly applying apoptotic cell-derived extracellular microvesicles to one of a naïve T cell culture or a dendritic cell culture, isolating antigen specific T cells from said one of a T cell culture and a dendritic cell culture, and storing said isolated antigen specific T cells.

Abstract: A system and method for using cold atmospheric plasma to treat an air flow to inactivate viruses and sterilize the air flow. The system comprises a source of a carrier gas, a humidifier connected to said source of a carrier gas, a source of a feed gas, a plasma generator configured to plasmatize said carrier gas into a plasma; and a mixer having an interior chamber, an active electrode inside said interior chamber and connected to an electrical output of said plasma generator, an outer electrode connected to a ground and a dielectric barrier between said inner electrode and said outer electrode, wherein said mixer has a first fluid input port connected to said source of a carrier gas, a second fluid input connected to said source of a feed gas and a fluid output connected to a ventilation system.

Abstract: A method for treating cancer by treating target cancer cells with cold atmospheric plasma to induce apoptosis of the target cancer cells through the degradation of Histone RNA during S-phase induced by oxidation of RNA.

Abstract: A method for bioengineering exosomes for cancer treatment. The method comprises the steps of isolating target tumor cells from a tissue sample, culturing the isolated target tumor cells, treating the cultured target tumor cells with cold atmospheric plasma to release exosomes from the target tumor cells, and collecting the released exosomes. The step of culturing the target tumor cells may comprise seeding the isolated target tumor cells in wells and incubating the target tumor cells in a culture media, for example, for 24 hours. The culture media may comprise Dulbecco's Modified Eagle Medium, an antibiotic and 10% fetal bovine serum. The step of culturing the target tumor cells may further comprise replacing the culture media with Dulbecco's Modified Eagle Medium and an antibiotic. The target tumor cells may comprise, for example, one of U87 cells, KKU-055 cells and triple negative MDA-MB-231 cells.

Abstract: A method for using Cold Atmospheric Plasma based T cell therapy for treating cancer including treating tumor cells with cold atmospheric plasma to permeabilize the tumor cells, condensing a mammalian CRISPR plasmid vector into compact nanostructures using one of poly-L-lysine (PLL) and Star-shaped poly(ethylene glycol)-block-polyethylenimine, and transferring into the tumor cells catalytically inactive Cas9 (dCas9) containing transcriptional activators (VP64-p65-Rta) paired with single guide RNAs (sgRNAs) and plasmid DNA (CRISPRa system) to elicit immune responses by enhancing the presentation of tumor-associated antigens.

Abstract: A system for performing cold atmospheric plasma treatment of respiratory infections or lung cancer having a source of a carrier gas, a cold atmospheric plasma generator connected to the source of carrier gas, a source of compressed air, a humidifier connected to the source of compressed air, a source of oxygen, a ventilator having inputs connected to an output of the humidifier and the source of oxygen, a mixer having an interior chamber formed from a dielectric, an active electrode inside the interior chamber, and an outer electrode connected to ground, wherein the mixer has a fluid input port connected to a gas output of the cold atmospheric plasma generator and an output of the ventilator, and a delivery member connected to an output of the mixer for delivering combined humidified air and cold atmospheric plasma to a respiratory system of a patient.

Abstract: A system for performing cold atmospheric plasma treatment of respiratory infections or lung cancer having a source of a carrier gas, a cold atmospheric plasma generator connected to the source of carrier gas, a source of compressed air, a humidifier connected to the source of compressed air, a source of oxygen, a ventilator having inputs connected to an output of the humidifier and the source of oxygen, a mixer having an interior chamber formed from a dielectric, an active electrode inside the interior chamber, and an outer electrode connected to ground, wherein the mixer has a fluid input port connected to a gas output of the cold atmospheric plasma generator and an output of the ventilator, and a delivery member connected to an output of the mixer for delivering combined humidified air and cold atmospheric plasma to a respiratory system of a patient.

Abstract: A method for performing cold atmospheric plasma treatment of respiratory infections or lung cancer having a source of a carrier gas, a cold atmospheric plasma generator connected to the source of carrier gas, a source of compressed air, a humidifier connected to the source of compressed air, a source of oxygen, a ventilator having inputs connected to an output of the humidifier and the source of oxygen, a mixer having an interior chamber formed from a dielectric, an active electrode inside the interior chamber, and an outer electrode connected to ground, wherein the mixer has a fluid input port connected to a gas output of the cold atmospheric plasma generator and an output of the ventilator, and a delivery member connected to an output of the mixer for delivering combined humidified air and cold atmospheric plasma to a respiratory system of a patient.

Abstract: A method for treatment of breast cancer using molecular profiling. The method comprises performing a molecular analysis of target breast cancer cells, identifying markers associated with the target breast cancer cells based on the performed molecular analysis, selecting on a graphical user interface of a cold atmospheric plasma generator the identified markers associated with the target breast cancer cells, selecting with a processor in the cold atmospheric plasma generator preferred cold atmospheric plasma settings associated with the identified markers in a database stored in a storage in the cold atmospheric plasma generator, and applying cold atmospheric plasma with the cold atmospheric plasma generator at the selected cold atmospheric pressure settings to the target breast cancer cells.

Abstract: A method for treatment of solid cancer tumors. The method includes pre-operatively treating a patient having a solid cancer tumor with at least one of chemotherapy and radiation therapy, surgically removing the solid cancer tumor from the patient, applying cold atmospheric plasma to the surgical margins surrounding the area in the patient from which the solid cancer tumor was removed, performing at least one of intra-operative chemotherapy or radiation therapy, and post-operatively performing at least one of chemotherapy and radiation therapy on the patient.

Abstract: A method for isolation and culturing of T Lymphocytes from whole blood and harvesting exosomes. The method comprises isolating peripheral blood mononuclear cells from human blood, culturing the isolated peripheral blood mononuclear cells, treating the cultured peripheral blood mononuclear cells with cold atmospheric plasma for secretion of microvesicles, and harvesting microvesicles from the CAP-treated peripheral blood mononuclear cells.

Abstract: A method for a method for treatment of cholangiocarcinoma (CCA) with cold atmospheric plasma and Folfirinox. The method includes treating a patient having CCA with a low dosage FOLFIRINOX regimen, for example, 6.73 nM, giving the patient the low-dosage FOLFIRINOX regimen pre-operatively, surgically removing the cancerous tumor from the patient, continuing the low-dosage FOLFIRINOX treatment regimen intra-operatively, applying cold atmospheric plasma to the surgical margins surrounding the area in the patient from which the tumor was removed, and continuing the FOLFIRINOX post-operatively.

Abstract: A method for treating cancer. The method comprises treating a patient having a cancerous tumor with a gene inhibitor pre-operatively to inhibit upregulation of a particular gene, surgically removing the cancerous tumor, treating the patient with the gene inhibitor intra-operatively to inhibit upregulation of a particular gene, applying cold atmospheric plasma to surgical margins around the area in the patient from which the tumor was surgically removed, and treating the patient with the gene inhibitor post-operatively.

Abstract: A method for treating cancer by treating target cancer cells with cold atmospheric plasma to induce apoptosis of the target cancer cells through the degradation of Histone RNA during S-phase induced by oxidation of RNA.

Abstract: A method for treatment of cancer using Cold Atmospheric Plasma treatment combined with CAR-T Therapy.

Abstract: A method for applying cold atmospheric plasma treatment to target tissue comprising the steps of selecting through a graphical user interface a particular soft tissue sarcoma cell line associated with target tissue, retrieving, with said computing device, settings data associated with said selected soft tissue sarcoma cell line from a database of cell line data and associated settings data in a storage, and applying, with said computing device, said retrieved settings data to a cold atmospheric plasma system.

Abstract: Methods for separating and identifying nucleic acids utilize carbon coated magnetic beads. The method teaches that multivalent cations promote binding of single stranded nucleic acids to the beads and that the single stranded nucleic acids can be released with the addition of chelating agents that bind the multivalent cations such as EDTA. The method further teaches that fragile single stranded nucleic acids, such as RNA, can be stored on the surface of the beads. Lastly, the method also teaches that by iteratively adding complimentary DNA oligos, single stranded nucleic acids can be quantified or individually isolated using the carbon coated magnetic beads.