Abstract: Methods of harvesting proteins directly from bioreactors to avoid at several steps in the purification of recombinant drugs are disclosed.

Abstract: A single-use round flexible mixing bag for use in bioprocessing in which a fluid is received and agitated using an internal fluid-agitating element comprising a radial flow impeller driven by an internal pneumatic vane motor is disclosed. The bag may include an integral sparger and sensor receiver. Related methods are also disclosed.

Abstract: A septum is positioned within a disposable vessel and defines a lower chamber and an upper chamber. The septum includes a plurality of apertures that provide fluid communication between the upper chamber and lower chamber. Compressed gas is introduced in the lower chamber to produce fine bubbles rising up throughout the vessel to produce a mixing and gasification needed for the growth of a biological culture and manufacture of a biological product in a nutrient medium. Adding a binding resin to the upper chamber allows harvesting, separation and purification of biological products in the reactor as a single unit operation.

Abstract: A felt-hat that does not fly off in the wind having vents in the brim camouflaged by a porous or solid material is disclosed.

Abstract: A method and a system for aging wines and spirits is disclosed using finely pulverized wood of less than 1 mm size and in such quantity to achieve equivalent aging in one-tenth to one-hundredth of the time required for traditional barrel aging and for instant aging prior to drinking.

Abstract: A non-blocking filtration system wherein the suspension filtered continuously scrubs the filter, keeping it free of deposits of solid deposits while the filtrate is removed in a variety of biological and chemical applications, substantially reducing the cost of unit operations.

Abstract: Large-scale downstream processing of secreted recombinant proteins is provided in a single device, wherein the contents of a plurality of bioreactors are combined simultaneous to their harvesting and purification resulting in significant savings of time and the cost of manufacturing.

Abstract: An assembly capable of capturing and purifying expressed biological products during or at the end of a bioreaction cycle is disclosed wherein a binding resin is kept separated from the contents of the bioreactor allowing capturing, harvesting and purification of biological products in a bioreactor; the invention additionally provides means of removing undesirable metabolic products as well as provides for efficient loading of chromatography columns.

Abstract: Single-use closed bioreactors with recirculating exhaust gas that can be operated in an uncontrolled environment are reported for the manufacturing of biological products using genetically modified biological cultures that produces carbon dioxide or that requires carbon dioxide in their metabolic process.

Abstract: A pair or receptacles capable of housing an emitter probe and a detector probe are installed inside a bioreactor to monitor the properties of the nutrient media without contacting the nutrient media.

Abstract: A chromatography column that captures components in a process liquid in a free flow state and allows elution in steps is described.

Abstract: A buoyant device containing chromatography media performs the function of protein harvesting replacing the steps of cell separation and volume reduction; the device can be loaded into columns for further purification.

Abstract: A single-pass HVAC systems to isolate zones and to maintain a required clean air quality standard is provided that operates by producing a positive pressure in all zones, while exhausting on a minimal quantity of air required by law. The zones are kept clean by a recirculating fan filter in each zone. The exhaust air is used to exchange heat with incoming air to conserve energy further.

Abstract: A method and an apparatus for separating suspended matter from liquid includes a concentrator filter that draws the liquid out of the suspension while the filter kept unblocked by a sparging filter that allows scrubbing of the concentrator filter by gas bubbles. This invention can be used to replace cross-flow filtration and centrifugation in the bioprocess industry and to reduce the volume of suspensions to concentrate the yield of the end product in the chemical industry.

Abstract: An air scrubber for eliminating an associated airborne contaminants and sterilizing air provided to protect against nocosomial infections, environmental allergens, weapons of biological and chemical attacks and operations requiring clean environment. The air scrubber includes a housing containing an alkali solution at pH 14 through which air passes and suspended liquid particles removed; provides are made for use in central air-conditioning systems, stand-alone applications and portable use along with respirators.

Abstract: Reactors that allow mixing and gasification by converting the entire floor of the reactor vessel to a sparge filter is described.

Abstract: Stationary bioreactors and mixing vessels fitted with single-use flexible bags utilizing wave hydrodynamic principle are described for use in every type of biological process and products.

Abstract: A method of combining the content of bioreactors allowing homogeneous mixing in accordance with CFR21 is described that allows processing of variable size batches using a single validated bioreactor is described.

Abstract: An exhaust system suitable for high volume exhaust from flexible disposable bags is described that prevents nutrient media volume loss and prevents cross-contamination without using any filters. The invention described here allows the use of disposable two-dimensional bioreactors for the cultivation of bacterial and other organisms and cells require high aeration.

Abstract: A bioreactor that combines the steps of recombinant expression and separation of a biological product by binding the secreted biological product with a resin, discarding the nutrient medium and eluting the biological product as a concentrated solution, eliminating the steps of sterile filtration and volume reduction. The method also allows loading of resin for column-purification, eliminating all steps of perfusion process and maintaining a sink condition of a toxic product in nutrient medium to optimize productivity of host cells. The instant invention also allows harvesting of solubilized inclusion bodies after the cells have been lysed and refolding of proteins inside the bioreactor.