Abstract: The present invention relates to a system for renal puncturing assistance in percutaneous nephrolithotomy (PCNL) surgery. The system includes a robotic device having an end effect or including an image acquisition unit, needle holding mechanism and a guide wire mechanism. The needle holding mechanism accommodates a puncture needle with corresponding guide wire provided by the guide wire mechanism. The system includes a computing unit operatively coupled to the image acquisition unit. The system has an AR engine that augments the pre-operative CT scan data of the patient with real time intra-operative images acquired using the image acquisition unit during the PCNL surgery, and is used along with an artificial intelligence engine for any or a combination of: identifying kidney and rib position of the patient, predicting an optimal puncture path for the PCNL surgery, and predicting an entry point on the marker patch for the PCNL surgery.

Abstract: The present disclosure relates to microfluidic cartridge for determining resistance or susceptibility of microorganism towards agent selected from a group comprising antibiotics, antiviral, antifungal, pesticide and insecticide. The disclosure further relates to a kit comprising microfluidic cartridge containing components selected from a group comprising growth media, agent, reagent, sensor and any combination thereof. The microfluidic cartridge and the kit described herein enables rapid and personalized determination of susceptibility or resistance of microorganisms towards agent. The disclosure also relates to an assay for determining the susceptibility or resistance of microorganisms towards agent employing the said microfluidic cartridge.

Abstract: The method provides a hyaluronic-acid binding based technique to separate functionally competent and mature sperm. The method for extracting viable sperms from a semen sample comprises incubating functionalized HA with functionalized paramagnetic beads to obtain a plurality of HA coated paramagnetic beads. The method further comprises incubating the plurality of HA coated paramagnetic beads with the semen sample to obtain a first population and a second population of sperm. The first population comprises sperm bound to the plurality of HA coated paramagnetic beads and the second population comprises sperms not bound to the plurality of HA coated paramagnetic beads. The method comprises magnetically separating the first population from the second population and separating sperms from the plurality of HA coated paramagnetic beads in the separated first population to obtain the viable sperms.

Abstract: The method provides a hyaluronic-acid binding based technique to separate functionally competent and mature sperm. The method for extracting viable sperms from a semen sample comprises incubating functionalized HA with functionalized paramagnetic beads to obtain a plurality of HA coated paramagnetic beads. The method further comprises incubating the plurality of HA coated paramagnetic beads with the semen sample to obtain a first population and a second population of sperm. The first population comprises sperm bound to the plurality of HA coated paramagnetic beads and the second population comprises sperms not bound to the plurality of HA coated paramagnetic beads. The method comprises magnetically separating the first population from the second population and separating sperms from the plurality of HA coated paramagnetic beads in the separated first population to obtain the viable sperms.