Abstract: Described herein are compositions and methods for the production and quantification of barcoded or unique molecular identifier (UMI)-labeled substrates. In one aspect, the substrate is a bead comprising a template oligonucleotide that is elongated by successive extension reactions to provide a bead with an oligonucleotide comprising a plurality of barcodes and conserved anchor regions. Methods are also described for quantifying the amount of template oligonucleotide loaded onto the substrate and the products of the extension reaction after each round and after the final extension.

Abstract: This invention relates to mutant Taq DNA polymerase having an enhanced template discrimination activity compared with an unmodified Taq DNA polymerase of SEQ ID NO.:1, wherein the amino acid sequence of the mutant Taq DNA polymerase consists of substitutions at residue positions 783, 784, or a combination of 783 and 784 of the unmodified Taq DNA polymerase of SEQ ID NO.:1.

Abstract: This invention relates to mutant DNA polymerases having an enhanced template discrimination activity compared with the corresponding unmodified DNA polymerase counterparts, wherein the amino acid sequence of the mutant DNA polymerase includes at least one substitution at residue positions structurally and functionally homologous or orthologous positions 783 or 784 of an unmodified Taq DNA polymerase.

Abstract: Methods and compositions are provided for improving specificity during amplification of a target DNA sequence. The methods and compositions rely upon the use of an RNase H enzyme, a polymerase, and RNase H enzyme-sensitive, blocked-cleavable oligonucleotide primers in the amplification reactions, wherein the reaction mixtures include either an optimized final concentration of a divalent metal salt comprising 2.0 mM or less of free Mg++ cation and/or an optimized final concentration of a non-ionic detergent comprising at least about 0.001% polyethylene glycol hexadecyl ether.

Abstract: The invention is directed to an in vitro method for joining a first set of double-stranded (ds) DNA molecules. Small molecules acting as chaperone agents are identified that promote efficient and rapid assembly (that is, joining) of overlapping double-stranded DNA fragments.

Abstract: This invention relates to mutant DNA polymerases having an enhanced template discrimination activity compared with the corresponding unmodified DNA polymerase counterparts, wherein the amino acid sequence of the mutant DNA polymerase includes at least one substitution at residue positions structurally and functionally homologous or orthologous positions 783 or 784 of an unmodified Taq DNA polymerase.

Abstract: The invention provides a provides improvements to assays that employ RNase H cleavage for biological applications related to nucleic acid amplification and detection, where the RNase H has been reversibly inactivated.

Abstract: The present invention provides methods of cleaving a nucleic acid strand to initiate, assist, monitor or perform biological assays.

Abstract: An electronic device is provided with an integral transducer used to update a random data pool without connection to an external source of new random data. In one embodiment, there is provided a method for providing random data on an electronic device, the method comprising: storing random data in a random data pool in a memory; sensing motion of the electronic device using a transducer integrally carried as part of the electronic device, wherein the transducer is configured to generate an output in response to sensed motion; generating new random data in accordance with the output of a transducer when sensed motion exceeds a threshold; and storing the new random data in the random data pool in the memory.

Abstract: Methods and compositions are provided for improving specificity during amplification of a target DNA sequence. The methods and compositions rely upon the use of an RNase H enzyme, a polymerase, and RNase H enzyme-sensitive, blocked-cleavable oligonucleotide primers in the amplification reactions, wherein the reaction mixtures include either an optimized final concentration of a divalent metal salt comprising 2.0 mM or less of free Mg++ cation and/or an optimized final concentration of a non-ionic detergent comprising at least about 0.001% polyethylene glycol hexadecyl ether.

Abstract: The present invention provides methods that more accurately predict melting temperatures for duplex oligomers. The invented methods predict the Tm of chimeric duplexes containing various amounts of locked nucleic acid modifications in oligonucleotide strands.

Abstract: This invention relates to mutant DNA polymerases having an enhanced template discrimination activity compared with the corresponding unmodified DNA polymerase counterparts, wherein the amino acid sequence of the mutant DNA polymerase includes at least one substitution at residue positions structurally and functionally homologous or orthologous positions 783 or 784 of an unmodified Taq DNA polymerase.

Abstract: An electronic device is provided with an integral transducer used to update a random data pool without connection to an external source of new random data. In one embodiment, there is provided a method for providing random data on an electronic device, the method comprising: storing random data in a random data pool in a memory; sensing motion of the electronic device using a transducer integrally carried as part of the electronic device, wherein the transducer is configured to generate an output in response to sensed motion; generating new random data in accordance with the output of a transducer when sensed motion exceeds a threshold; and storing the new random data in the random data pool in the memory.

Abstract: The invention provides a provides improvements to assays that employ RNase H cleavage for biological applications related to nucleic acid amplification and detection, where the RNase H has been reversibly inactivated.

Abstract: An electronic device is provided with an integral transducer used to update a random data pool without connection to an external source of new random data. In one embodiment, there is provided a method for providing random data on an electronic device, the method comprising: storing random data in a random data pool in a memory; sensing motion of the electronic device using a transducer integrally carried as part of the electronic device, wherein the transducer is configured to generate an output in response to sensed motion; generating new random data in accordance with the output of a transducer when sensed motion exceeds a threshold; and storing the new random data in the random data pool in the memory.

Abstract: The present invention provides methods of cleaving a nucleic acid strand to initiate, assist, monitor or perform biological assays.

Abstract: The invention provides a provides improvements to assays that employ RNase H cleavage for biological applications related to nucleic acid amplification and detection, where the RNase H has been reversibly inactivated.

Abstract: A wireless personal area network (WPAN) system includes a plurality of WPAN devices using encrypted wireless communication therebetween when in an enabled state and not wirelessly communicating when in a disabled state. At least one of the WPAN devices includes a WPAN identity reader for reading at least one identifying parameter of a user, for confirming that the user is an authorized user based upon reading the at least one identifying parameter, and for wirelessly communicating with at least one other WPAN device to switch the at least one other WPAN device from the disabled state to the enabled state based upon confirming the user is an authorized user.

Abstract: A reader device includes a housing for receiving a smart card, a processor in electronic communication with an integrated circuit chip of the smart card when the smart card is received in the housing, a wireless communications device in electronic communication with the processor for enabling the reader device to transmit first encrypted information wirelessly and to receive second encrypted information wirelessly, and a memory in electronic communication with the processor that includes one or more routines executable by the processor. The one or more routines include a cryptographic module adapted to encrypt first information to create the first encrypted information and decrypt the second encrypted information to obtain second information. In addition, a communication system that includes the reader device and a computing device, wherein the reader device and computing device are able to wirelessly exchange information in a secure manner.

Abstract: A mobile communication device is provided with an integral transducer used to refresh a random data pool without connection to an external source of new random data.