Abstract: This disclosure provides methods for quantifying individual amino acids in various bodily fluids obtained from a human patient. Also provided are reference ranges for normal amino acid levels in the various bodily fluids (e.g., blood plasma, urine, cerebrospinal fluid, and saliva) and for various age groups (e.g., neonates, infants, children, and adults).

Abstract: Mass spectrometric methods are described for determining the amount of analyte in a sample collected by a microsampling device. Provided herein are methods directed to quantitating the amount of an analyte in a sample by extracting an analyte from a sample collected by a microsampling device, purifying the sample by liquid chromatography, ionizing the analyte to generate one or more ions detectable by mass spectrometry; and determining the amount of the one or more ions by mass spectrometry. The amount of analyte in the sample is related to the amount of analyte in the patient.

Abstract: The invention relates to the detection of fatty acids. In a particular aspect, the invention relates to methods for detecting very long chain fatty acids and branched chain fatty acids by mass spectrometry.

Abstract: The invention relates to the detection of fatty acids. In a particular aspect, the invention relates to methods for detecting very long chain fatty acids and branched chain fatty acids by mass spectrometry.

Abstract: A method of authenticating a user so that the user can access a website without entering a unique user credential. A user accesses a target and is presented with an authentication code and an address, and the user sends a message containing the authentication code to that address. Alternatively, the user is pre-supplied with an address and is presented only with an authentication code when the user accesses the target. The user's identity is authenticated by comparing an aspect of the metadata of the message with known metadata aspects, and the user is authenticated by comparing the authentication key presented to the user with the one received in the message. Both the user and the user's identity are authenticated in a single step without requiring the user to input any unique user credential.

Abstract: A method of authenticating a user so that the user can access a website without entering a unique user credential. A user accesses a target and is presented with an authentication code and an address, and the user sends a message containing the authentication code to that address. Alternatively, the user is pre-supplied with an address and is presented only with an authentication code when the user accesses the target. The user's identity is authenticated by comparing an aspect of the metadata of the message with known metadata aspects, and the user is authenticated by comparing the authentication key presented to the user with the one received in the message. Both the user and the user's identity are authenticated in a single step without requiring the user to input any unique user credential.

Abstract: Systems and method for authenticating users are presented. A system can send a passkey to a user interface of a known device. A user can then send a messaging service message with the passkey from a second device to the system. After receiving the message from the user, the system can extract the passkey from the message, and compare the received passkey against the passkey originally sent to the user. The known device and the second device can each have separate and unique device identifiers.

Abstract: This disclosure provides methods for quantifying individual amino acids in various bodily fluids obtained from a human patient. Also provided are reference ranges for normal amino acid levels in the various bodily fluids (e.g., blood plasma, urine, cerebrospinal fluid, and saliva) and for various age groups (e.g., neonates, infants, children, and adults).

Abstract: Systems and method for authenticating users are presented. A system can send a passkey to a user interface of a known device. A user can then send a messaging service message with the passkey from a second device to the system. After receiving the message from the user, the system can extract the passkey from the message, and compare the received passkey against the passkey originally sent to the user. The known device and the second device can each have separate and unique device identifiers.

Abstract: The invention relates to the detection of fatty acids. In a particular aspect, the invention relates to methods for detecting very long chain fatty acids and branched chain fatty acids by mass spectrometry.

Abstract: The invention relates to the detection of fatty acids. In a particular aspect, the invention relates to methods for detecting very long chain fatty acids and branched chain fatty acids by mass spectrometry.

Abstract: Systems and method for authenticating users are presented. A system can send a passkey to a user interface of a known device. A user can then send a messaging service message with the passkey from a second device to the system. After receiving the message from the user, the system can extract the passkey from the message, and compare the received passkey against the passkey originally sent to the user. The known device and the second device can each have separate and unique device identifiers.

Abstract: The invention relates to the detection of fatty acids. In a particular aspect, the invention relates to methods for detecting very long chain fatty acids and branched chain fatty acids by mass spectrometry.

Abstract: This disclosure provides methods for quantifying individual amino acids in various bodily fluids obtained from a human patient. Also provided are reference ranges for normal amino acid levels in the various bodily fluids (e.g., blood plasma, urine, cerebrospinal fluid, and saliva) and for various age groups (e.g., neonates, infants, children, and adults).

Abstract: A method for detecting amino acids in body fluids is described. The method includes derivatizing the body fluid amino acids (e.g. plasma amino acids), separating the derivatized amino acids by liquid chromatography followed by and subjecting the identification by mass spectrometry. The identity of each amino acid from the body fluid is determined by comparing to a set of structurally similar amino acid standards, which preferably are added to the body fluid sample as internal standards. Use of the method of diagnose an individual with a metabolic disorder is also provided.

Abstract: An implant for deployment in select locations or select tissue for regeneration of tissue is disclosed. The implant comprising collagen and or other bio-resorbable materials, where the implant may also be used for therapy delivery. Additionally, the implant may be “matched” to provide the implant with similar physical and/or chemical properties as the host tissue.

Abstract: A method for detecting amino acids in body fluids is described. The method includes derivatizing the body fluid amino acids (e.g. plasma amino acids), separating the derivatized amino acids by liquid chromatography followed by and subjecting the identification by mass spectrometry. The identity of each amino acid from the body fluid is determined by comparing to a set of structurally similar amino acid standards, which preferably are added to the body fluid sample as internal standards. Use of the method of diagnose an individual with a metabolic disorder is also provided.

Abstract: An implantable device for facilitating the healing of voids in bone, cartilage and soft tissue is disclosed. A preferred embodiment includes a cartilage region comprising a polyelectrolytic complex joined with a subchondral bone region. The cartilage region, of this embodiment, enhances the environment for chondrocytes to grow articular cartilage; while the subchondral bone region enhances the environment for cells which migrate into that region's macrostructure and which differentiate into osteoblasts. Another embodiment is arranged for the local delivery of therapeutic agent. A preferred embodiment is a porous resorbable implant, wherein the therapy delivery may be localized in nature, rather than systemic, such that higher doses at the target site may be allowed than would be tolerable by the body systemically.

Abstract: An implantable device for facilitating the healing of voids in bone, cartilage and soft tissue is disclosed. A preferred embodiment includes a cartilage region comprising a polyelectrolytic complex joined with a subchondral bone region. The cartilage region, of this embodiment, enhances the environment for chondrocytes to grow articular cartilage; while the subchondral bone region enhances the environment for cells which migrate into that region's macrostructure and which differentiate into osteoblasts. A hydrophobic barrier exists between the regions, of this embodiment. In one embodiment, the polyelectrolytic complex transforms to hydrogel, following the implant procedure.

Abstract: Porous polymers having a plurality of openings or chambers that are highly convoluted, with each chamber being defined by multiple, thin, flat partitions are produced by a new gel enhanced phase separation technique. In a preferred embodiment, a second liquid is added to a polymer solution, the second liquid causing the solution to increase in viscosity. With sufficient polymer and second liquid present, the increase in viscosity can be up to that of a gel. The gel can then be shaped as needed. Subsequent solvent extraction leaves the porous polymeric body of defined shape. The porous polymers have utility as medical prostheses, the porosity permitting ingrowth of neighboring tissue. A second material may be incorporated into the chambers, thereby creating a microstructure filling the voids of the macrostructure.