Patents by Inventor Scott J. Geromanos
Scott J. Geromanos has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11887700Abstract: Techniques and apparatus for generating encoded representations of compounds are described. In one embodiment, for example, an apparatus may include at least one memory, and logic coupled to the at least one memory. The logic may be configured receive analytical information associated with at least one compound, generate at least one encoded representation of the at least one compound, the encoded representation comprising at least one segment representing at least one property of the at least one compound using a plurality of symbols. Other embodiments are described.Type: GrantFiled: May 4, 2020Date of Patent: January 30, 2024Assignee: WATERS TECHNOLOGIES IRELAND LIMITEDInventors: Steven J. Ciavarini, Curt Devlin, Patrick Brophy, Scott J. Geromanos
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Publication number: 20230307091Abstract: The exemplary embodiments may obtain protein, gene sequence or nucleic acid sequences from data sources, process the sequences with a processor executing computer programming instructions to identify mutations and display information regarding the mutations to a user on a display device. For protein sequences, the exemplary embodiments may identify which subsequences in the sequences are well suited for observing as positions in the sequences where possible mutations may arise. Statistical techniques may be applied to variations in the subsequences to determine whether the variations are mutations or not. The displayed information regarding each mutation may include the nature of the mutation, the frequency of the mutation, the location of the user having the mutation, the date that the sample was obtained and other information of interest.Type: ApplicationFiled: March 22, 2023Publication date: September 28, 2023Inventors: Scott J. Geromanos, Emmy Maria Hoyes, Francis Tracey, Allen Caswell, Ming Gao
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Publication number: 20210333251Abstract: Exemplary embodiments provide methods, mediums, and systems for analyzing spectrometry and/or chromatography data, and in particular to techniques to improve the reproducibility of results of spectrographic and/or chromatographic experiments. For example, some embodiments provide techniques for normalizing mass spectrometry (MS) and/or liquid chromatography (LC) data across different experimental devices, allowing data from different cohorts to be directly compared. To this end, exemplary embodiments provide a reliable, reproducible target library usable across different platforms, laboratories, and users. One embodiment leverages statistical techniques to select experimental parameters configured to reduce or minimize the chance of misidentifying a target molecule. Another embodiment leverages the law of large numbers to produce a composite product ion spectrum usable across different experiments.Type: ApplicationFiled: April 23, 2021Publication date: October 28, 2021Inventors: Scott J. Geromanos, Steven J. Ciavarini, Marisa Gioioso, Ovidiu Pop
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Patent number: 11137380Abstract: A method of analyzing a complex sample includes performing a sequential chromatographic-IMS-MS analysis of a sample to obtain a plurality of experimental mass spectra having isotopic clusters, wherein each spectrum of the plurality of spectra is associated with a chromatographic retention time and an ion-mobility drift time. The method also includes calculating a model isotopic cluster of a precursor or product ion associated with a candidate compound in the sample, in correspondence to the natural isotopic-abundance ratios of elements composing the compound. The method further includes comparing peaks of the model isotopic cluster to corresponding peaks of an isotopic cluster of one of the experimental mass spectra to extract one or more saturated or interfered peaks of the experimental isotopic cluster, wherein at least one of the peaks of the experimental isotopic cluster is un-saturated and un-interfered.Type: GrantFiled: May 19, 2020Date of Patent: October 5, 2021Assignee: Waters Technologies CorporationInventors: Scott J. Geromanos, Marc V. Gorenstein, Daniel Golick, Steven J. Ciavarini
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Patent number: 11011359Abstract: Techniques for performing data acquisition and analysis are described. A multi-mode acquisition strategy may be performed which iteratively selects mass isolation windows of different sizes in different scan cycles to acquire experimental data. The mass isolation windows selected may provide for acquiring elevated energy scan data for a defined set of m/z values. Single scan data analysis may be performed. Data analysis may include forming precursor charge clusters, chaining precursor charge clusters having the same mass to charge ratio to form peaks profiles, and using criteria to align precursor and product ions of the experimental data. Unsupervised and supervised clustering may be performed using a database and composite ion spectra formed from experimental data. Also described are a small molecule acquisition enhancement and additional techniques applicable for biopharmaceutical and other applications.Type: GrantFiled: January 17, 2020Date of Patent: May 18, 2021Assignee: Waters Technologies CorporationInventors: Scott J. Geromanos, Steven J. Ciavarini, Curt Devlin
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Publication number: 20200350039Abstract: Techniques and apparatus for generating encoded representations of compounds are described. In one embodiment, for example, an apparatus may include at least one memory, and logic coupled to the at least one memory. The logic may be configured receive analytical information associated with at least one compound, generate at least one encoded representation of the at least one compound, the encoded representation comprising at least one segment representing at least one property of the at least one compound using a plurality of symbols. Other embodiments are described.Type: ApplicationFiled: May 4, 2020Publication date: November 5, 2020Inventors: Steven J. Ciavarini, Curt Devlin, Patrick Brophy, Scott J. Geromanos
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Patent number: 10825672Abstract: Techniques and apparatus for analyzing mass spectrometry data are described. In one embodiment, for example, an apparatus may include logic to access a product ion data set generated via mass analyzing a sample comprising a target precursor, access precursor composition information for elements of the target precursor that includes nominal mass and mass defect information, determine nominal mass (NM)-mass defect (MD) relationship information for ion fragments associated with the target precursor based on the precursor composition information, determine one or more fragment upper boundaries and one or more fragment lower boundaries, extract candidate ion fragments from the ion fragments via applying the one or more fragment upper boundaries and the one or more fragment lower boundaries to the NM-MD relationship information, and determine target ion fragments from the plurality of candidate ion fragments based on fragmentation efficiency information associated with the candidate ion fragments.Type: GrantFiled: November 21, 2017Date of Patent: November 3, 2020Assignee: WATERS TECHNOLOGIES CORPORATIONInventors: Scott J. Geromanos, Curt Devlin, Steven J. Ciavarini
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Publication number: 20200279726Abstract: A method of analyzing a complex sample includes performing a sequential chromatographic-IMS-MS analysis of a sample to obtain a plurality of experimental mass spectra having isotopic clusters, wherein each spectrum of the plurality of spectra is associated with a chromatographic retention time and an ion-mobility drift time. The method also includes calculating a model isotopic cluster of a precursor or product ion associated with a candidate compound in the sample, in correspondence to the natural isotopic-abundance ratios of elements composing the compound. The method further includes comparing peaks of the model isotopic cluster to corresponding peaks of an isotopic cluster of one of the experimental mass spectra to extract one or more saturated or interfered peaks of the experimental isotopic cluster, wherein at least one of the peaks of the experimental isotopic cluster is un-saturated and un-interfered.Type: ApplicationFiled: May 19, 2020Publication date: September 3, 2020Inventors: Scott J. Geromanos, Marc V. Gorenstein, Daniel Golick, Steven J. Ciavarini
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Publication number: 20200266042Abstract: Techniques for performing data acquisition and analysis are described. A multi-mode acquisition strategy may be performed which iteratively selects mass isolation windows of different sizes in different scan cycles to acquire experimental data. The mass isolation windows selected may provide for acquiring elevated energy scan data for a defined set of m/z values. Single scan data analysis may be performed. Data analysis may include forming precursor charge clusters, chaining precursor charge clusters having the same mass to charge ratio to form peaks profiles, and using criteria to align precursor and product ions of the experimental data. Unsupervised and supervised clustering may be performed using a database and composite ion spectra formed from experimental data. Also described are a small molecule acquisition enhancement and additional techniques applicable for biopharmaceutical and other applications.Type: ApplicationFiled: January 17, 2020Publication date: August 20, 2020Inventors: Scott J. Geromanos, Steven J. Ciavarini, Curt Devlin
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Patent number: 10692709Abstract: A method of analyzing a complex sample includes performing a sequential chromatographic-IMS-MS analysis of a sample to obtain a plurality of experimental mass spectra having isotopic clusters, wherein each spectrum of the plurality of spectra is associated with a chromatographic retention time and an ion-mobility drift time. The method also includes calculating a model isotopic cluster of a precursor or product ion associated with a candidate compound in the sample, in correspondence to the natural isotopic-abundance ratios of elements composing the compound. The method further includes comparing peaks of the model isotopic cluster to corresponding peaks of an isotopic cluster of one of the experimental mass spectra to extract one or more saturated or interfered peaks of the experimental isotopic cluster, wherein at least one of the peaks of the experimental isotopic cluster is un-saturated and un-interfered.Type: GrantFiled: October 29, 2018Date of Patent: June 23, 2020Assignee: Waters Technologies CorporationInventors: Scott J. Geromanos, Marc V. Gorenstein, Daniel Golick, Steven J. Ciavarini
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Patent number: 10573501Abstract: Techniques for performing data acquisition and analysis are described. A multi-mode acquisition strategy may be performed which iteratively selects mass isolation windows of different sizes in different scan cycles to acquire experimental data. The mass isolation windows selected may provide for acquiring elevated energy scan data for a defined set of m/z values. Single scan data analysis may be performed. Data analysis may include forming precursor charge clusters, chaining precursor charge clusters having the same mass to charge ratio to form peaks profiles, and using criteria to align precursor and product ions of the experimental data. Unsupervised and supervised clustering may be performed using a database and composite ion spectra formed from experimental data. Also described are a small molecule acquisition enhancement and additional techniques applicable for biopharmaceutical and other applications.Type: GrantFiled: May 31, 2016Date of Patent: February 25, 2020Assignee: WATERS TECHNOLOGIES CORPORATIONInventors: Scott J. Geromanos, Steven J. Ciavarini, Curt Devlin
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Patent number: 10495647Abstract: A method of mass spectrometry comprises ionising a sample eluting from a separation device in order to generate a plurality of parent ions. Multiple cycles of operation are performed as the sample elutes from the separation device. Each cycle of operation comprises mass analysing the parent ions to obtain parent ion mass spectral data, and mass analysing fragment or product ions derived from the parent ion to obtain fragment or product ion mass spectral data. Each cycle of operation also comprises mass analysing fragment or product ions derived from parent ions having mass to charge ratios within a first range to obtain first fragment or product ion mass spectral data, and mass analysing fragment or product ions derived from parent ions having mass to charge ratios within a second different range to obtain second fragment or product ion mass spectral data. The method can provide a hybrid data independent acquisition (DIA) and data dependent acquisition (DDA) approach.Type: GrantFiled: June 12, 2015Date of Patent: December 3, 2019Assignee: Waters Technologies CorporationInventors: Scott J Geromanos, Steven J Ciavarini, James I. Langridge, Brad J Williams, Michael J Nold
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Publication number: 20190131117Abstract: A method of analyzing a complex sample includes performing a sequential chromatographic-IMS-MS analysis of a sample to obtain a plurality of experimental mass spectra having isotopic clusters, wherein each spectrum of the plurality of spectra is associated with a chromatographic retention time and an ion-mobility drift time. The method also includes calculating a model isotopic cluster of a precursor or product ion associated with a candidate compound in the sample, in correspondence to the natural isotopic-abundance ratios of elements composing the compound. The method further includes comparing peaks of the model isotopic cluster to corresponding peaks of an isotopic cluster of one of the experimental mass spectra to extract one or more saturated or interfered peaks of the experimental isotopic cluster, wherein at least one of the peaks of the experimental isotopic cluster is un-saturated and un-interfered.Type: ApplicationFiled: October 29, 2018Publication date: May 2, 2019Inventors: Scott J. Geromanos, Marc V. Gorenstein, Daniel Golick, Steven J. Ciavarini
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Patent number: 10242853Abstract: A method of mass spectrometry comprises ionizing a sample eluting from a separation device in order to generate a plurality of parent ions. The method further comprises generating a target list of ions, which includes a predicted mass to charge ratio, a predicted chromatographic retention or elution time, and a predicted ion mobility drift time, derived from a model. Multiple cycles of operation are then performed as the sample elutes from the separation device. Each cycle of operation includes mass filtering the parent ions so that selected ions having mass to charge ratios within a first mass to charge ratio range are onwardly transmitted to a fragmentation or reaction device. The target list is then checked and the model is updated accordingly. The first mass to charge ratio range can then be adjusted in response to the updated model.Type: GrantFiled: June 12, 2015Date of Patent: March 26, 2019Assignee: Waters Technologies CorporationInventors: Scott J Geromanos, Steven J Ciavarini, Weibin Chen, Stephane Houel
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Patent number: 10115576Abstract: A method of analyzing a complex sample includes performing a sequential chromatographic-IMS-MS analysis of a sample to obtain a plurality of experimental mass spectra having isotopic clusters, wherein each spectrum of the plurality of spectra is associated with a chromatographic retention time and an ion-mobility drift time. The method also includes calculating a model isotopic cluster of a precursor or product ion associated with a candidate compound in the sample, in correspondence to the natural isotopic-abundance ratios of elements composing the compound. The method further includes comparing peaks of the model isotopic cluster to corresponding peaks of an isotopic cluster of one of the experimental mass spectra to extract one or more saturated or interfered peaks of the experimental isotopic cluster, wherein at least one of the peaks of the experimental isotopic cluster is un-saturated and un-interfered.Type: GrantFiled: December 8, 2014Date of Patent: October 30, 2018Assignee: WATERS TECHNOLOGIES CORPORATIONInventors: Scott J. Geromanos, Marc V. Gorenstein, Daniel Golick, Steven J. Ciavarini
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Publication number: 20180166265Abstract: Techniques for performing data acquisition and analysis are described. A multi-mode acquisition strategy may be performed which iteratively selects mass isolation windows of different sizes in different scan cycles to acquire experimental data. The mass isolation windows selected may provide for acquiring elevated energy scan data for a defined set of m/z values. Single scan data analysis may be performed. Data analysis may include forming precursor charge clusters, chaining precursor charge clusters having the same mass to charge ratio to form peaks profiles, and using criteria to align precursor and product ions of the experimental data. Unsupervised and supervised clustering may be performed using a database and composite ion spectra formed from experimental data. Also described are a small molecule acquisition enhancement and additional techniques applicable for biopharmaceutical and other applications.Type: ApplicationFiled: May 31, 2016Publication date: June 14, 2018Inventors: Scott J. Geromanos, Steven J. Ciavarini, Curt Devlin
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Publication number: 20180144918Abstract: Techniques and apparatus for analyzing mass spectrometry data are described. In one embodiment, for example, an apparatus may include logic to access a product ion data set generated via mass analyzing a sample comprising a target precursor, access precursor composition information for elements of the target precursor that includes nominal mass and mass defect information, determine nominal mass (NM)-mass defect (MD) relationship information for ion fragments associated with the target precursor based on the precursor composition information, determine one or more fragment upper boundaries and one or more fragment lower boundaries, extract candidate ion fragments from the ion fragments via applying the one or more fragment upper boundaries and the one or more fragment lower boundaries to the NM-MD relationship information, and determine target ion fragments from the plurality of candidate ion fragments based on fragmentation efficiency information associated with the candidate ion fragments.Type: ApplicationFiled: November 21, 2017Publication date: May 24, 2018Inventors: Scott J. Geromanos, Curt Devlin, Steven J. Ciavarini
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Patent number: 9810669Abstract: Techniques are described for quantification of molecules in a sample. Mass spectrometry is performed to obtain ionization intensities for precursor and product ions originating from a particular molecule. A first stet of precursor ions having the highest ionization intensities and originating from the particular molecule is determined. For each of the one or precursors in the first set, determined is a second set of one or more product ions that are fragments associated with said each precursor and have the highest ionization in intensities of product ions associated with said each precursor. An intensity sum is calculated for the particular molecule by adding ionization intensities of product ions included in the second sets for the one or more precursors in the first set. The intensity sum is compared to information included in a calibration standard. A quantity of the particular molecule in the sample is determined based on said comparing.Type: GrantFiled: November 16, 2012Date of Patent: November 7, 2017Assignee: Waters Technologies CorporationInventors: Scott J. Geromanos, Johannes P C Vissers, James I. Langridge
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Patent number: 9734996Abstract: A method of mass spectrometry comprises ionizing a sample and obtaining mass spectral data relating to a plurality of ion detection events. The method then comprises applying match tolerances for mass to charge ratio (m/z), chromatographic retention time (tr), and ion mobility drift time (td), to the ion detection events in order to determine possible charge state connections. The method also comprises constructing a tentative isotope chain and querying ion detection events for a match to the tentative isotope chain.Type: GrantFiled: June 12, 2015Date of Patent: August 15, 2017Assignee: Waters Technologies CorporationInventors: Scott J Geromanos, Steven J Ciavarini
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Publication number: 20170125223Abstract: A method of mass spectrometry comprises ionising a sample eluting from a separation device in order to generate a plurality of parent ions. The method further comprises generating a target list of ions, which includes a predicted mass to charge ratio, a predicted chromatographic retention or elution time, and a predicted ion mobility drift time, derived from a model. Multiple cycles of operation are then performed as the sample elutes from the separation device. Each cycle of operation includes mass filtering the parent ions so that selected ions having mass to charge ratios within a first mass to charge ratio range are onwardly transmitted to a fragmentation or reaction device. The target list is then checked and the model is updated accordingly. The first mass to charge ratio range can then be adjusted in response to the updated model.Type: ApplicationFiled: June 12, 2015Publication date: May 4, 2017Inventors: Scott J GEROMANOS, Steven J CIAVARINI, Weibin CHEN, Stephane HOUEL