Abstract: The present invention relates to a method for recovering a protein of interest from a bacterial fermentation broth, wherein the bacterial cells in the fermentation broth exhibit a high degree of lysis, comprising the steps of adding to the fermentation broth comprising the protein of interest at least one flocculant in an amount of 0.5 to 50 g/l of the fermentation broth; separating the protein of interest from at least the bacterial cells by filtration, preferably by dead-end filtration or microfiltration; and thereby obtaining the protein of interest in the filtrate; wherein the bacterial cells are Bacillus cells. Further, the invention relates to the use of a flocculant for recovering a protein of interest from a bacterial fermentation broth comprising a high degree of lysed cells.

Abstract: The present invention relates to a method for separating a molecule of interest from an antifoaming agent in a fermentation broth comprising said molecule of interest and said antifoaming agent. The method comprises the steps of filtering the fermentation broth at a temperature in the range of 1° C. to 15° C. above the cloud point of the antifoaming agent, and thereby obtaining a first fraction of the fermentation broth comprising the molecule of interest and a second fraction of the fermentation broth comprising the antifoaming agent, wherein the antifoaming agent is a polyalkylene glycol based antifoaming agent. The invention further relates to a process for purifying a molecule of interest from a fermentation broth comprising said method.

Abstract: The present invention relates to a method for recovering a protein of interest from a fermentation broth by adding a salt of a divalent cation and increasing the pH.

Abstract: Polymer capsule comprising at least one polymer P1 and at least one microorganism M, wherein said polymer P1 has a solubility in water at 21° C. of at least 1 g/l and wherein said polymer capsule has an average particle size d90 of below 100 ?m, wherein said microorganism M is distributed throughout said capsule.

Abstract: The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or a modified NADH-dependent variant thereof, wherein said KARI is at least about 80% identical to SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 28, SEQ ID NO: 40, or SEQ ID NO: 58. The present invention also relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or a modified NADH-dependent variant thereof, wherein said KARI is at least about 99% identical to SEQ ID NO: 64. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol.