Abstract: A self-circularization RNA construct that can be expressed in a DNA vector and simultaneously circularized through a self-targeting and splicing reaction to form a circRNA is disclosed. The circRNA can consist only of a gene of interest which can be a coding, non-coding, or a combination thereof. The gene of interest has the advantage of being able to rapidly express a peptide or protein. The formed circRNA has a circular structure and has a stable and high half-life because 5? and 3? ends are not exposed. Accordingly, functional RNA such as miRNA, anti-miRNA, siRNA, shRNA, aptamer, functional RNA for gene/RNA editing, ADAR (adenosine deaminase acting on the RNA)-recruiting RNA, mRNA vaccine, mRNA therapeutic agent, vaccine adjuvant, and CAR-T mRNA can be produced as a stable circRNA in cells.

Abstract: An advertising method on a network comprises displaying a guide code corresponding to a network address of an advertisement object and allowing a user to transmit said guide code. Moreover, an advertisement method on a network for additionally allows a user to purchase an advertised good or service by transmitting a guide code via a mobile telephone and charging the cost of the purchase to a calling account of the mobile telephone. Also, the present invention has particular application as an advertisement guide system and method using guide codes, wherein users are able to simply and conveniently recollect Internet homepage addresses of advertisers of advertisements made through various advertisement media, using voice or character mailboxes or electronic mails of mobile communication terminals, without having to write down the Internet homepage addresses on a memo paper.