Patents by Inventor Sergei Svitashev
Sergei Svitashev has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20240084318Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell, without incorporating a selectable transgene marker. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed, without incorporating a selectable transgene marker. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a guide polynucleotide/Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell, without incorporating a selectable transgene marker.Type: ApplicationFiled: July 20, 2023Publication date: March 14, 2024Applicants: CORTEVA AGRISCIENCE LLC, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, MICHAEL LASSNER, ZHAN-BIN LIU, SERGEI SVITASHEV
-
Publication number: 20230323374Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.Type: ApplicationFiled: November 15, 2022Publication date: October 12, 2023Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INCInventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, L. ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
-
Patent number: 11773400Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell, without incorporating a selectable transgene marker. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed, without incorporating a selectable transgene marker. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a guide polynucleotide/Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell, without incorporating a selectable transgene marker.Type: GrantFiled: August 20, 2014Date of Patent: October 3, 2023Inventors: Andrew Mark Cigan, Saverio Carl Falco, Michael Lassner, Zhan-Bin Liu, Sergei Svitashev
-
Publication number: 20230279413Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.Type: ApplicationFiled: November 16, 2022Publication date: September 7, 2023Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INCInventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, L. ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
-
Publication number: 20230235345Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.Type: ApplicationFiled: November 17, 2022Publication date: July 27, 2023Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INCInventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, L. ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
-
Publication number: 20230235342Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.Type: ApplicationFiled: August 23, 2022Publication date: July 27, 2023Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANYInventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, MICHAEL LASSNER, DEREK JANTZ, ZHONGSEN LI, ZHAN-BIN LIU, SERGEI SVITASHEV, JAMES JEFFERSON SMITH
-
Publication number: 20230203517Abstract: Compositions and methods are provided for large scale manipulation of genomic regions and chromosomal engineering of plant genomes. Portions of chromosomes may be deleted, translocated, duplicated or inverted, which are useful for various applications in plant breeding programs that relate to recombination, crossovers, and genetic gain. Site-specific directed DNA breaks enhance targeted recombination frequencies, crossover efficiency and movement of large chromosomal segments in crop plant cells. CRISPR-Cas systems enable targeted chromosomal engineering of crop plants.Type: ApplicationFiled: May 28, 2021Publication date: June 29, 2023Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: MARY J. FRANK, JUSTIN P. GERKE, JENELLE D. F. MEYER, SERGEI SVITASHEV
-
Publication number: 20230091338Abstract: Compositions and methods are provided for an inducible, high efficiency intra-genomic homologous recombination (IGHR) system in plants, that can be used for non-chimeric, heritable gene targeting. The advantage of IGHR approach to targeted integration is that every cell contains donor DNA and nuclease-encoding sequences, so that there are many potentially homology-directed targeting events that can occur during plant development.Type: ApplicationFiled: February 22, 2021Publication date: March 23, 2023Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: AJITH ANAND, MAREN L ARLING, PIERLUIGI BARONE, HYEON-JE CHO, WILLIAM JAMES GORDON-KAMM, SANDEEP KUMAR, BRIAN LENDERTS, KEITH S LOWE, SERGEI SVITASHEV, XINLI EMILY WU
-
Publication number: 20230059309Abstract: Compositions and methods are provided for the excision and replacement of an endogenous polynucleotide, such as a gene, using CRISPR-Cas systems. In some aspects, the gene is flanked by specific nucleotides that are targets of homology-directed repair, for the insertion of a replacement polynucleotide.Type: ApplicationFiled: January 5, 2021Publication date: February 23, 2023Applicant: Pioneer Hi-Bred International, Inc.Inventors: Huirong GAO, Sergei SVITASHEV
-
Patent number: 11459576Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.Type: GrantFiled: July 10, 2020Date of Patent: October 4, 2022Inventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Michael Lassner, Derek Jantz, Zhongsen Li, Zhan-Bin Liu, Sergei Svitashev, James Jefferson Smith
-
Publication number: 20220307006Abstract: Methods and compositions are provided for the improvement of homology-directed repair of a double strand break in a plant cell, using concatemers of heterologous polynucleotides that are flanked by sequences capable of sequence hybridization with a guide RNA. In some aspects, the double strand break is created by an RNA-guided Cas endonuclease. The homology-directed repair of the double-strand break may include incorporation of a heterologous polynucleotide, for example a gene encoding a trait of agronomic importance. The homology-directed repair of the double-strand break may occur as a result of template-directed repair using a heterologous polynucleotide as a repair template.Type: ApplicationFiled: July 17, 2020Publication date: September 29, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: AMIT DAS, SATISH KANUGANTI, AMITABH MOHANTY, SERGEI SVITASHEV, VENKATA S. TAVVA
-
Publication number: 20220251587Abstract: Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.Type: ApplicationFiled: April 11, 2022Publication date: August 11, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: STEPHEN LAWRENCE GASIOR, WILLIAM JAMES GORDON-KAMM, KEITH S LOWE, DAVID J PETERSON, SERGEI SVITASHEV, JOSHUA K YOUNG
-
Patent number: 11332752Abstract: Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.Type: GrantFiled: March 11, 2019Date of Patent: May 17, 2022Assignee: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Stephen Lawrence Gasior, William James Gordon-Kamm, Keith S Lowe, David J Peterson, Sergei Svitashev, Joshua K Young
-
Publication number: 20210062201Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.Type: ApplicationFiled: July 10, 2020Publication date: March 4, 2021Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANYInventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, MICHAEL LASSNER, DEREK JANTZ, ZHONGSEN LI, ZHAN-BIN LIU, SERGEI SVITASHEV, JAMES JEFFERSON SMITH
-
Publication number: 20210010012Abstract: Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.Type: ApplicationFiled: March 11, 2019Publication date: January 14, 2021Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: STEPHEN LAWRENCE GASIOR, WILLIAM JAMES GORDON-KAMM, KEITH S LOWE, DAVID J PETERSON, SERGEI SVITASHEV, JOSHUA K YOUNG
-
Patent number: 10822610Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.Type: GrantFiled: June 13, 2018Date of Patent: November 3, 2020Inventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Michael Lassner, Derek Jantz, Zhongsen Li, Zhan-Bin Liu, Sergei Svitashev, James Jefferson Smith
-
Publication number: 20200157554Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.Type: ApplicationFiled: January 30, 2020Publication date: May 21, 2020Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, L. ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
-
Publication number: 20190040372Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.Type: ApplicationFiled: October 18, 2018Publication date: February 7, 2019Applicants: E. I. DUPONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Ericka Bermudez, Andrew Mark Cigan, James J English, Saverio Carl Falco, Huirong Gao, Lu Liu, Zhan-Bin Liu, Azalea S Ong, Sergei Svitashev, Joshua K Young
-
Publication number: 20190040405Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.Type: ApplicationFiled: September 13, 2018Publication date: February 7, 2019Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, LESZEK ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
-
Publication number: 20180371479Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.Type: ApplicationFiled: August 3, 2018Publication date: December 27, 2018Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Zhongsen Li, Zhan-Bin Liu, L. Aleksander Lyznik, Jinrui Shi, Sergei Svitashev, Joshua K. Young