Patents by Inventor Sergey G. Lokhov

Sergey G. Lokhov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Modified thymine polynucleotide oligomers and methods
    Patent number: 9598455
    Abstract: Disclosed are modified thymine bases that provide enhanced base-pairing affinity for adenine or 2,6-diaminopurine bases in polynucleotide hybridization complexes. Also disclosed are polynucleotide oligomers, polynucleotide hybridization complexes that comprise such modified thymine bases. Also disclosed are various methods of use. For example, in some embodiments, modified polynucleotide oligomers disclosed herein can be used as primers and probes for nucleic acid amplification and/or detection.
    Type: Grant
    Filed: March 30, 2015
    Date of Patent: March 21, 2017
    Assignee: Cepheid
    Inventors: Alexander A. Gall, Sergey G. Lokhov, Mikhail A. Podyminogin, Ekaterina V. Viazovkina, Kevin Patrick Lund
  • MODIFIED THYMINE POLYNUCLEOTIDE OLIGOMERS AND METHODS
    Publication number: 20150275278
    Abstract: Disclosed are modified thymine bases that provide enhanced base-pairing affinity for adenine or 2,6-diaminopurine bases in polynucleotide hybridization complexes. Also disclosed are polynucleotide oligomers, polynucleotide hybridization complexes that comprise such modified thymine bases. Also disclosed are various methods of use. For example, in some embodiments, modified polynucleotide oligomers disclosed herein can be used as primers and probes for nucleic acid amplification and/or detection.
    Type: Application
    Filed: March 30, 2015
    Publication date: October 1, 2015
    Inventors: Alexander A. GALL, Sergey G. LOKHOV, Mikhail A. PODYMINOGIN, Ekaterina V. VIAZOVKINA, Kevin Patrick LUND
  • MODIFIED CYTOSINE POLYNUCLEOTIDE OLIGOMERS AND METHODS
    Publication number: 20150275283
    Abstract: Disclosed are modified cytosine bases that provide enhanced base-pairing affinity for guanine bases in polynucleotide hybridization complexes. Also disclosed are polynucleotide oligomers, polynucleotide hybridization complexes that comprise such modified cytosine bases. Also disclosed are various methods of use. For example, in some embodiments, modified polynucleotide oligomers disclosed herein can be used as primers and probes for nucleic acid amplification and/or detection.
    Type: Application
    Filed: March 30, 2015
    Publication date: October 1, 2015
    Inventors: Alexander A. GALL, Sergey G. Lokhov, Mikhail A. Podyminogin, Ekaterina V. Viazovkina, Kevin Patrick Lund
  • TM leveling methods
    Patent number: 7751982
    Abstract: Methods for designing an oligonucleotide sequence having a selected duplex stability are provided wherein the oligonucleotide portion comprises at least one modified base selected from universal bases, unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally having attached minor groove binders and reporter groups.
    Type: Grant
    Filed: September 26, 2003
    Date of Patent: July 6, 2010
    Assignee: Elitech Holding B.V.
    Inventors: Robert O. Dempcy, Alexander A. Gall, Sergey G. Lokhov, Irina A. Afonina, Igor V. Kutyavin, Nicolaas M. J. Vermeulen
  • Single nucleotide polymorphism analysis of highly polymorphic target sequences
    Patent number: 7718374
    Abstract: Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.
    Type: Grant
    Filed: January 28, 2008
    Date of Patent: May 18, 2010
    Assignee: Elitech Holding B.V.
    Inventors: Yevgeniy Belousov, Robert O. Dempcy, Sergey G. Lokhov, Alexei Vorobiev
  • Systems and methods for predicting oligonucleotide melting temperature (TS)
    Patent number: 7715989
    Abstract: Software systems and methods for predicting the melting temperature (Tm) and other characteristics of oligonucleotides, including modified oligonucleotides. Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
    Type: Grant
    Filed: June 18, 2002
    Date of Patent: May 11, 2010
    Assignee: Elitech Holding B.V.
    Inventors: Robert O. Dempcy, Alexander A. Gall, Sergey G. Lokhov, Irina A. Afonina, Michael J. Singer, Karen M. Singer, legal representative, Igor V. Kutyavin, Nicolaas M. J. Vermeulen
  • SINGLE NUCLEOTIDE POLYMORPHISM ANALYSIS OF HIGHLY POLYMORPHIC TARGET SEQUENCES
    Publication number: 20090087922
    Abstract: Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.
    Type: Application
    Filed: January 28, 2008
    Publication date: April 2, 2009
    Applicant: Epoch Biosciences, Inc.
    Inventors: Yevgeniy Belousov, Robert O. Dempcy, Sergey G. Lokhov, Alexei Vorobiev
  • Real-time linear detection probes: sensitive 5'-minor groove binder-containing probes for PCR analysis
    Patent number: 7485442
    Abstract: Oligonucleotide probes/conjugates are provided along with method for their use in assays to monitor amplification wherein the signal produced does not rely on 5? nuclease digestion.
    Type: Grant
    Filed: June 19, 2006
    Date of Patent: February 3, 2009
    Assignee: Epoch Biosciences, Inc.
    Inventors: Irina A. Afonina, Yevgeniy S. Belousov, Robert O. Dempcy, Igor V. Kutyavin, Sergey G. Lokhov, Eugeny A. Lukhtanov
  • Tleveling compositions
    Patent number: 7368549
    Abstract: Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
    Type: Grant
    Filed: April 19, 2005
    Date of Patent: May 6, 2008
    Assignee: Epoch Biosciences, Inc.
    Inventors: Robert O. Dempcy, Alexander A Gall, Sergey G. Lokhov, Irina A. Afonina, Michael J. Singer, Igor V. Kutyavin, Nicolaas M. J. Vermeulen
  • Single nucleotide polymorphism analysis of highly polymorphic target sequences
    Patent number: 7348146
    Abstract: Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.
    Type: Grant
    Filed: September 29, 2004
    Date of Patent: March 25, 2008
    Assignee: Epoch Biosciences, Inc.
    Inventors: Yevgeniy Belousov, Robert O. Dempcy, Sergey G. Lokhov, Alexei Vorobiev
  • Real-time linear detection probes: sensitive 5?-minor groove binder-containing probes for PCR analysis
    Patent number: 7205105
    Abstract: Oligonucleotide probes/conjugates are provided along with method for their use in assays to monitor amplification wherein the signal produced does not rely on 5? nuclease digestion.
    Type: Grant
    Filed: June 6, 2002
    Date of Patent: April 17, 2007
    Assignee: Epoch Biosciences, Inc.
    Inventors: Irina A. Afonina, Yevgeniy S. Belousov, Robert O. Dempcy, Igor V. Kutyavin, Sergey G. Lokhov, Eugeny A. Lukhtanov
  • Modified oligonucleotides for mismatch discrimination
    Patent number: 7045610
    Abstract: Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
    Type: Grant
    Filed: February 28, 2001
    Date of Patent: May 16, 2006
    Assignee: Epoch Biosciences, Inc.
    Inventors: Robert O. Dempcy, Alexander A. Gall, Sergey G. Lokhov, Irina A. Afonina, Michael J. Singer, Igor V. Kutyavin, Nicolaas M. J. Vermeulen
  • Tm leveling methods
    Patent number: 6683173
    Abstract: Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
    Type: Grant
    Filed: December 21, 2001
    Date of Patent: January 27, 2004
    Assignee: Epoch Biosciences, Inc.
    Inventors: Robert O. Dempcy, Alexander A. Gall, Sergey G. Lokhov, Irina A. Afonina, Michael J. Singer, Igor V. Kutyavin, Nicolaas M. J. Vermeulen
  • Systems and methods for predicting oligonucleotide melting temperature (TmS)
    Publication number: 20030235822
    Abstract: Software systems and methods for predicting the melting temperature (Tm) and other characteristics of oligonucleotides, including modified oligonucleotides. Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
    Type: Application
    Filed: June 18, 2002
    Publication date: December 25, 2003
    Applicant: Epoch Biosciences, Inc.
    Inventors: Sergey G. Lokhov, Igor V. Kutyavin, Robert O. Dempcy, Alexander A. Gall, Irina A. Afonina, Michael J. Singer, Nicolaas M.J. Vermeulen
  • TMLEVELING METHODS
    Publication number: 20030224359
    Abstract: Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.
    Type: Application
    Filed: December 21, 2001
    Publication date: December 4, 2003
    Applicant: Epoch Biosciences, Inc.
    Inventors: Robert O. Dempcy, Alexander A. Gall, Sergey G. Lokhov, Irina A. Afonina, Michael J. Singer, Igor V. Kutyavin, Nicolaas M.J. Vermeulen